Summary
A simple, rapid and effective capillary electrophoresis method was developed for the characterization of 10 different species ofMahonia. A fingerprint of the extract of each species was constructed using a mixed buffer of borate and phosphate, containing methanol, pH 8.0. The effective electrophoretic mobility and % normalized area of each peak in electrophoregrams were evaluated to characterize various species. Three alkaloids: berberine, palmatine and jatrorrhizine were found in all 10 species. The CE technique appears to provide a powerful tool for the identification and quality control of plant drugs.
Article PDF
Avoid common mistakes on your manuscript.
References
Z. M. Gu, S. Y. Chen, Zhong Yao Cai17, (1978).
Pharmacopoeia Committee (Editor), Pharmacopoeia of People’s Republic of China, Guangdong Sci. & Tech. Press, Guangzhou, 1990, p. 68.
P. G. Xiao, Zhongyao Tongbao7, 612 (1959).
Guha, Mukherjee, Lloydia42, 1 (1979).
G. H. Lu, J. M. Chen, L. W. Wang, P. G. Xiao, Huaxi Yaoxue Zazhi10, 202 (1995).
K. Galle, S. Bladt, H. Wagner, Dtsch. Apoth. Ztg.,134, 35 (1994).
M. L. Liu, H. L. Feng, Zhongcaoyao22, 308 (1991).
S. Xu, Huaxue54, 55 (1996).
Y. M. Liu, S. J. Sheu, J. Chromatogr. A623, 196 (1992).
Y. M. Liu, S. J. Sheu, J. Chromatogr. A639, 323 (1993).
G. H. Zhang, Y. Z. Wang, Y. Y., Zhang, Chinese Journal of Chromatography13, 247 (1995).
K. L. Li, S. J. Sheu, Anal Chim Acta303, 113 (1995).
Y. M. Liu, S. J. Sheu, J. Chromatogr.600, 370 (1992).
Y. M. Liu, S. J. Sheu, J. Chromatogr.634, 329 (1993).
J. G. Tian, J. Ren, H. X. Lou, Y. H. Gao, Zhongguo Zhongyao Zazhi23, 263 (1998).
K. D. Altria, Chromatographia35, 177 (1993).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Li, Y., Ji, X., Liu, H. et al. Characterization of 10 species ofMahonia by capillary electrophoresis. Chromatographia 51, 357–361 (2000). https://doi.org/10.1007/BF02490617
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02490617