Abstract
Mycelial growth of an isolate ofT. bakamatsutake was tested in media with C/N ratio ranging from 0 to 50 and with 32 carbon and 12 nitrogen sources. The isolate grew best at the C/N ratio of 30. It utilized the monosaccharidesd-glucose,d-mannose, andd-fructose, the disaccharide trehalose, and polysaccharide pectin among the carbon sources; and yeast extract,l-glutamic acid, and ammonium compounds among the nitrogen sources. The growth of ten isolates and secretion of gluconic and oxalic acids were compared ind-glucose, trehalose, and pectin media. The utilization ofd-glucose, trehalose, and pectin differed among the ten isolates, but all the isolates secreted gluconic acid in thed-glucose media and oxalic acid in the pectin media.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Literature cited
Cromack, K., Jr., Sollins, P., Graustein, W., Speidel, K., Todd, A. W., Spycher, G., Lie, C. Y. and Todd, R. L. 1979. Calcium oxalate accumulation and soil weathering in mats of the hypogeous fungusHysterangium crassum. Soil Biol. Biochem.11: 463–468.
Duchesne, L. C., Ellis, B. E. and Peterson, R. L. 1989. Disease suppression by the ectomycorrhizal fungusPaxillus involutus: contribution of oxalic acid. Can. J. Bot.67: 2776–2730.
Hacskaylo, E. 1973. Carbohydrate physiology of ectomycorrhizae. In: Ectomycorrhizae. Their ecology and physiology, (ed. by Marks, G. C. and Kozlowski, T. T.), pp. 207–230. Academic Press, New York.
Harley, J. L. and Smith, S. E. 1983. Mycorrhizal symbiosis, pp. 146–154. Academic Press, London.
Hutchison, L. J. 1990. Studies on the systematics of ectomycorrhizal fungi in axenic culture. II. The enzymatic degradation of selected carbon and nitrogen compounds. Can. J. Bot.68: 1522–1530.
Iwase, K. 1992. Gluconic acid synthesis by the ectomycorrhizal fungusTricholoma robustum. Can. J. Bot.70: 84–88.
Jakobsen, I. 1996. Carbon metabolism in mycorrhiza. In: Methods in microbiology, vol. 23. Techniques for the study of mycorrhiza, (ed. by Norris, J. R., Read, D. J. and Varma, A. K.) pp. 149–180. Academic Press, London.
Kawai, M. and Abe, S. 1976. Studies on the artificial reproduction ofTricholoma matsutake (S. Ito et Imai) Sing. I. Effects of carbon and nitrogen sources in media on the vegetative growth ofT. matsutake. Trans. Mycol. Soc. Japan17: 159–167. (In Japanese.)
Lapeyrie, F., Chilvers, G. A. and Bhem, C. A. 1987. Oxalic acid synthesis by the mycorrhizal fungusPaxillus involutus (Batsch. ex Fr.) Fr. New Phytol.106: 139–146.
Malajczuk, N. and Cromack K., Jr. 1982. Accumulation of calcium oxalate in the mantle of ectomycorrhizal roots ofPinus radiata andEucalyptus marginata. New Phytol.92: 527–531.
Molina, R. and Palmer, J. G. 1982. Isolation, maintenance, and pure culture manipulation of ectomycorrhizal fungi. In: Methods and principles of mycorrhizal research, (ed. by Schenck, N. C.) pp. 115–129. Amer. Phytopathol. Soc., Minnesota.
Mouly, A., Rumeau, D. and Tugayé, M. T. E. 1992. Differential accumulation of hydroxyproline-rich glycoprotein transcripts in sunflower plants infected withSclerotinia sclerotiorum or treated with oxalic acid. Plant Sci.85: 51–59.
Norkrans, B. 1950. Studies in growth and cellulolytic enzymes ofTricholoma. Symb. Bot. Upsal.11: 1–126.
Norkrans, B. 1953. The effect of glutamic acid, aspartic acid, and related compounds on the growth of certainTricholoma species. Physiol. Plant.6: 584–593.
Ohta, A. 1990. A new medium for mycelial growth of mycorrhizal fungi. Trans. Mycol. Soc. Japan31: 323–334.
Palmer, J. G. and Hacskaylo, E. 1970. Ectomycorrhizal fungi in pure culture 1. Growth on single carbon sources. Physiol. Plant.23: 1187–1197.
Shimada, M. 1993. Oxalic acid metabolism of wood-destroying Basidiomycetes: Toward biochemistry of wood protection. Wood Res. Tech. Note29: 1–18. (In Japanese.)
Smith, S. E. and Read, D. J. 1996. Mycorrhizal symbiosis, 2nd ed., pp. 233–275. Academic Press, London.
Taber, W. A. and Taber, R. A. 1987. Carbon nutrition and respiration ofPisolithus tinctorius. Trans. Br. Mycol. Soc.89: 13–26.
Terashima, Y. 1994. Change in medium components and colony morphology due to mycelial growth of ectomycorrhizal fungusTricholoma bakamatsutake. Mycoscience35: 153–159.
Terashima, Y. and Mizoguchi, T. 1995. Nutritional environment of soil and roots in and around mycelial blocks of an ectomycorrhizal fungusTricholoma bakamatsutake in an evergreen Fagaceae forest. Mycoscience36: 167–172.
Author information
Authors and Affiliations
About this article
Cite this article
Terashima, Y. Carbon and nitrogen utilization and acid production by mycelia of the ectomycorrhizal fungusTricholoma bakamatsutake in vitro. Mycoscience 40, 51–56 (1999). https://doi.org/10.1007/BF02465673
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02465673