Summary
The effect of ovarian insufficiency on calcium metabolism has been thought to involve an increased bone resorptive effect of parathyroid hormone and possible impaired synthesis of 1,25-dihydroxyvitamin D3. In the present study a rat model allowing for controlled serum levels of parathyroid hormone and 1,25-dihydroxyvitamin D3 was used. Oophorectomy in this species is associated with increased serum levels of 1,25-dihydroxyvitamin D3 and decreased bone mass. Although thyroparathyroidectomy increased bone mass, an increased sensitivity of bone to parathyroid hormone in oophorectomized rats was not observed. Thus the development of the osteopenia did not seem to be related to increased parathyroid hormone sensitivity or to reduced levels of 1,25-dihydroxyvitamin D3. Exogenous 1,25-dihydroxyvitamin D3 increased bone mass in oophorectomized as well as intact rats. Intestinal calcium transport was increased by moderate doses of 1,25-dihydroxyvitamin D3. Intestinal calcium transport was also reduced by thyroparathyroidectomy and increased by the administration of parathyroid extract. A tendency for increased accumulation of 1,25-dihydroxyvitamin D3 in blood in oophorectomized rats has been noted. It is suggested that the tendency to hypercalcemia in ovarian-insufficient females given 1-hydroxylated vitamin D compounds may be related to a diminished metabolism of 1,25-dihydroxyvitamin D3.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Saville PD (1969) Changes in skeletal mass and fragility with castration in the rat: a model of osteoporosis. J Am Geriatr Soc 17:155–164
Lindgren JU, Lindholm TS (1979) Effect of 1-alpha-hydroxyvitamin D3 on osteoporosis in rats induced by oophorectomy. Calcif Tissue Int 27:161–164
Nordin BEC (1971) The clinical significance and pathogenesis of osteoporosis. Br Med J 1:571–576
Orimo H, Fujita T, Yoshikawa M (1972) Increased sensitivity of bone to parathyroid hormone in ovariectomized rats. Endocrinology 90:760–763
Atkins D, Zanelli JM, Peacock M, Nordin BED (1972) The effects of oestrogens on the response of bone to parathyroid hormonein vitro. J Endocrinol 54:107–174
Heaney RP (1976) Estrogens and postmenopausal osteoporosis. Clin Obstet Gynecol 19:791–794
Gallagher JC, Riggs BL, Eisman J, Hamstra A, Arnaud SB, DeLuca HF (1979) Intestinal calcium absorption and serum vitamin D metabolites in normal subjects and osteoporotic patients. Effect of age and dietary calcium. J Clin Invest 64:729–736
Suda T, DeLuca HF, Tanaka Y (1970) Biological activity of 25-hydroxyergocalciferol in rats. J Nutr 100:1049–1052
Steenbock H, Herting DC (1955) Vitamin D and growth. J Nutr 57:449–468
Lindquist B (1952) Effect of vitamin D on the metabolism of radiocalcium in rachitic rats. Acta Paediatr 41:Suppl 86
Chen PS Jr, Toribara TY, Warner H (1956) Microdetermination of phosphorus. Anal Chem 28:1756–1758
Shepard RM, Horst RL, Hamstra AJ, DeLuca HF (1979) Determination of vitamin D and its metabolites in plasma from normal and anephric man. Biochem J 182:55–69
Martin DL, DeLuca HF (1969) Influence of sodium on calcium transport by the rat small intestine. Am J Physiol 216:1351–1359
Bullamore JR, Gallagher JC, Wilkinson R, Marshall DH (1970) Radiocalcium measurement of bone turnover in disorders of calcium metabolism using a model based on an expanding pool. In Symposium on Dynamic Studies with Radioisotopes in Clinical Medicine and Research. Vienna J.A.E.A., pp 519–537
Garabedian M, Holick MF, DeLuca HF, Boyle IT (1972) control of 25-hydroxycholecalciferol metabolism by the parathyroid glands. Proc Natl Acad Sci USA 69:1673–1676
Tanaka Y, Castillo L, DeLuca HF (1976) Control of renal vitamin D hydroxylase in birds by sex hormones. Proc Natl Acad Sci USA 73:2701–2705
Marshall DH, Nordin BEC (1977) The effect of 1α-hydroxyvitamin D3 with and without oestrogens on calcium balance in postmenopausal women. Clin Endocrinol 7s:159–164
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lindgren, U., DeLuca, H.F. Role of parathyroid hormone and 1,25-dihydroxyvitamin D3 in the development of osteopenia in oophorectomized rats. Calcif Tissue Int 34, 510–514 (1982). https://doi.org/10.1007/BF02411294
Issue Date:
DOI: https://doi.org/10.1007/BF02411294