Summary
The metabolism of arachidonic acid to its cyclo-oxygenase products was studied in monolayer cultures of osteoblast-rich rat calvarial cells and of clonal cell lines from a rat osteogenic sarcoma, enriched in the osteoblast phenotype. Prostanoids were measured by radioimmunoassay after extraction of media and fractionation by high pressure liquid chromatography. In both normal and malignant osteoblasts the major cyclo-oxygenase product was 6-oxo-prostaglandin F1α, the hydration product of prostacyclin, with lesser amounts of prostaglandin E2 and prostaglandin F2α. No significant thromboxane B2 was detected. Prostaglandins are thought to have a local role in the regulation of bone resorption. These results point to the possible importance of prostacyclin either in bone resorption or in some other local function, e.g., regulation of bone blood flow.
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References
Klein DC, Raisz LG (1970) Prostaglandins: Stimulators of bone resorption in tissue culture. Endocrinology 86:1436–1440
Schelling SH, Wolfe HJ, Tashjian AH Jr (1980) Role of the osteoclast in prostaglandin E2-stimulated bone resorption. A correlative morphometric and biochemical analysis. Lab Invest 42:290–295
Tashjian AH Jr, Ivey JL, Delclos B, Levine L (1978) Stimulation of prostaglandin production in bone by phorbol diesters and melittin. Prostaglandins 16:221–232
Tashjian AH Jr, Levine L (1978) Epidermal growth factor stimulates prostaglandin production and bone resorption in cultured mouse calvaria. Biochem Biophys Res Commun 85:966–975
Raisz LG, Sandberg AL, Goodson JM, Simmons HA, Mergenhagen SE (1974) Complement-dependent stimulation of prostaglandin synthesis and bone resorption. Science 185:789–791
Raisz LG, Vanderhoek JY, Simmons HA, Kream BE, Nicholaou KC (1979) Prostaglandin synthesis by fetal rat bone in vitro: Evidence for a role of prostacyclin. Prostaglandins 17:905–914
Martin TJ, Atkins D (1979) Biochemical regulators of bone resorption and their significance in cancer. Essays Med Biochem 4:49–82
Martin TJ, Partridge NC (1980) Prostaglandins, cancer and bone: Pharmacological considerations. Metab Bone Dis Rel Res 2:167–171
Atkins D, Martin TJ (1977) Rat osteogenic sarcoma cells: Effects of some prostaglandins, their metabolites and analogues on cyclic AMP production. Prostaglandins 13:861–871
Partridge NC, Alcorn D, Michelangeli VP, Kemp BE, Ryan GB, Martin TJ (1981) Functional properties of hormonally responsive cultured normal and malignant rat osteoblastic cells. Endocrinology 108:213–219
Martin TJ, Partridge NC, Greaves M, Atkins D, Ibbotson KJ (1979) Prostaglandin effects on bone and role in cancer hypercalcaemia. In: MacIntyre I, Szelke M (eds) Molecular Endocrinology. Elsevier/North Holland, Amsterdam, pp 251–264
Rodan GA, Martin TJ (1981) Role of osteoblasts in hormonal control of bone resorption—a hypothesis. Calcif Tissue Int 33:349–351
Partridge NC, Frampton RJ, Eisman JA, Michelangeli VP, Elms E, Bradley TR, Martin TJ (1980) Receptors for 1,25(OH)2 vitamin D3 enriched in cloned osteoblast-like rat osteogenic sarcoma cells. FEBS Letters 115:139–142
Luben RA, Wong GL, Cohn DV (1976) Biochemical characterization with parathormone and calcitonin of isolated bone cells: Provisional identification of osteoclasts and osteoblasts. Endocrinology 99:526–533
Martin TJ, Ingleton PM, Underwood JCE, Michelangeli VP, Hunt NH, Melick RA (1976) Parathyroid hormone-responsive adenylate cyclase in induced transplantable osteogenic rat sarcoma. Nature 260:436–439
Martin TJ, Ingleton PM, Coulton LA, Melick RA (1979) Metabolic properties of hormonally responsive osteogenic sarcoma cells. Clin Orthop 140:247–254
Alam I, Ohuchi K, Levine L (1979) Determination of cyclo-oxygenase products and prostaglandin metabolites using high pressure liquid chromatography and radioimmunoassay. Anal Biochem 93:339–345
Nolan RD, Dusting GJ, Martin TJ (1981) Phospholipase inhibition and the mechanism of angiotensin-induced prostacyclin release from rat mesenteric vasculature. Biochem Pharmacol 30:2121–2125
Voelkel EF, Tashjian AH Jr, Levine L (1980) Cyclo-oxygenase products of arachidonic acid metabolism by mouse bone in organ culture. Biochim Biophys Acta 620:418–428
Raisz LG, Dietrich JW, Simmons HA, Seyberth HW, Hubbard W, Oates JA (1977) Effect of prostaglandin endoperoxides and metabolites on bone resorption in vitro Nature 267:532–534
Tashjian AH Jr, Tice JE, Sides K (1977) Biological activities of prostaglandin analogues and metabolites on bone in organ culture. Nature 266:645–647
Greaves M, Ibbotson KJ, Atkins D, Martin TJ (1980) Prostaglandins as mediators of bone resorption in renal and breast tumours. Clin Sci 58:201–210
Dowsett M, Easty GC, Powles TJ, Easty DM, Neville AM (1977) Human breast tumor-induced osteolysis and prostaglandins. Prostaglandins 11:447–460
Crawford A, Atkins D, Martin TJ (1978) Rat osteogenic sarcoma cells: Comparison of the effects of prostaglandins E1, E2, I2 (prostacyclin), 6 keto F1α, and thromboxane B2 on cyclic AMP production and adenylate cyclase activity. Biochim Biophys Res Commun 82:1195–1201
Partridge NC, Kemp BE, Veroni MC, Martin TJ (1981) Activation of adenosine 3′,5′-monophosphate-dependent protein kinase in normal and malignant bone cells by parathyroid hormone, prostaglandin E2 and prostacyclin. Endocrinology 108:220–225
Raisz LG, Simmons HA, Sandberg AL, Canalis E (1980) Direct stimulation of bone resorption by epidermal growth factor. Endocrinology 107:270–273
Hopkins NR, Gorman RR (1981) Regulation of endothelial cell cyclic nucleotide metabolism by prostacyclin. J Clin Invest 67:540–546
Gorman RR, Bunting S, Miller OV (1977) Modulation of human platelet adenylate cyclase by prostacyclin (PGX). Prostaglandins 13:377–388
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Nolan, R.D., Partridge, N.C., Godfrey, H.M. et al. Cyclo-oxygenase products of arachidonic acid metabolism in rat osteoblasts in culture. Calcif Tissue Int 35, 294–297 (1983). https://doi.org/10.1007/BF02405049
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DOI: https://doi.org/10.1007/BF02405049