Abstract
An allele of theProt locus, which encodes a major globulin of the maize scutellum, is a variant for a site of protein processing. Segregation analysis and recombination mapping indicate that the variant is an allele of theProt locus. DesignatedProt-V, this allele specifies three polypeptides, V1, V2, and V3. The V1 polypeptide is incompletely processed during the proteolytic processing step catalyzed by the product of theMep locus. Cyanogen bromide cleavage studies support the precursor-product relationship between V1 and V2. The V1 product is shortened with respect to other PROT′ proteins and it is postulated that the normal site of MEP processing has been removed by this foreshortening.
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Crouch, M. L., Tenbarge, K. M., Simon, A. E., and Ferl, R. (1983). cDNA clones forBrassica napus seed storage proteins: Evidence from nucleotide sequence analysis that both subunits of napin are cleaved from a precursor polypeptide.J. Mol. Appl. Genet. 2273.
Dierks-Ventling, C. (1982). Gobulins of developing maize seeds: Preliminary characterization. In Burger, M. M., and Weber, R. (eds.),Embryonic Development. B. Cellular Aspects Alan R. Liss, New York, p. 545.
Khavkin, E. E., Misharin, S. I., Markov, Y. Y., and Peshkov, A. A. (1978). Identification of embryonal antigens of maize: Globulins as primary reserve proteins of the embryo.Planta 14311.
Kriz, A. L., and Schwartz, D. (1986). Synthesis of globulins in maize embryos.Plant Physiol. 821069.
Laemmli, U. K. (1970). Cleavage of structural protein during the assembly of the head of bacteriophage T4.Nature 227680.
Lipp, J., and Dobberstein, B. (1986). The membrane-spanning segment of invariant chain (Iγ) contains a potentially cleavable signal sequence.Cell 461103.
Morrissey, J. H. (1981). Silver stain for proteins in polyacrylamide gels: A modified procedure with enhanced uniform sensitivity.Anal. Biochem. 117307.
Schwartz, D. (1979). Analysis of the size alleles of thePro gene in maize—Evidence for a mutant protein processor.Mol. Gen. Genet. 174233.
Schwartz, D., and Endo, T. (1966). Alcohol dehydrogenase polymorphism in maize—Simple and compound loci.Genetics 53709.
Spencer, D., Chandler, P. M., Higgins, T. J. V., Inglis, A. S., and Rubria, M. (1983). Sequence interrelationships of the subunits of vicilin from pea seeds.Plant Mol. Biol. 2259.
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This work was done with the support of United States Department of Agriculture Grant GM84-CRCR-1-1479.
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Osterman, J.C. An allele of theProt locus in maize is a variant for the site of protein processing. Biochem Genet 26, 463–474 (1988). https://doi.org/10.1007/BF02399413
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DOI: https://doi.org/10.1007/BF02399413