Abstract
Cassava (Manihot esculenta Crantz) was grown in the greenhouse and in the field at different levels of phosphorus applied, with or without inoculation with VA mycorrhiza in sterilized or unsterilized soil. When grown in a sterilized soil to which eight levels of P had been applied the non-inoculated plants required the application of 3200 kg P ha−1 to reach near-maximum yield of plant dry matter (DM) at 3 months. Inoculated plants, however, showed only a minor response to applied P. Mycorrhizal inoculation in the P check increased top growth over 80 fold and total P uptake over 100 fold.
Relating dry matter produced to the available P concentration in the soil (Bray II), a critical level of 15 ppm P was obtained for mycorrhizal and 190 ppm P for non-mycorrhizal plants. This indicates that the determination of critical levels of P in the soil is highly dependent on the degree of mycorrhizal infection of the root system. In a second greenhouse trial with two sterilized and non-sterilized soils it was found that in both sterilized soils, inoculation was most effective at intermediate levels of applied P resulting in a 15–30 fold increase in DM at 100 kg P ha−1. In the unsterilized soil inoculation had no significant effect in the quilichao soil, but increased DM over 3 fold in the Carimagua soil, indicating that the latter had a native mycorrhizal population less effective than the former.
When cassava was grown in the field in plots with 11 levels of P applied, uninoculated plants grown in sterilized soil remained extremely P deficient for 4–5 months after which they recuperated through mycorrhizal infection from unsterilized borders or subsoil. Still, after 11 months inoculation had increased root yields by 40%. In the non-sterilized soil inoculation had no significant effect as the introduced strain was equally as effective as the native mycorrhizal population.
These trials indicate that cassava is extremely dependent on an effective mycorrhizal association for normal growth in low-P soils, but that in most natural soils this association is rapidly established and inoculation of cassava in the field can only be effective in soils with a low quantity and quality of native mycorrhiza. In that case, plants should be inoculated with highly effective strains.
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References
Abbott L K and Robson A D 1977 Growth stimulation of subterranean clover with vesicular arbuscular mycorrhizas. Aust. J. Agric. Res. 28, 639–649.
Centro Internacional de Agricultura Tropical 1982 Cassava Program Annu. Rep. 1981.
Daft M J and Nicolson T H 1969 Effect of Endogone mycorrhiza on plant growth. II. Influence of soluble phosphate on endophyte and host in maize. New Phytol. 68, 945–952.
Guerrero R 1971 Soils of the Colombian Llanos Orientales-composition and classification of selected soil profiles. Unpublished Ph.D Thesis, Dept. Soil Science, North Carolina State Univ. Raleigh, N.C.
Hayman D S 1975 The occurrence of mycorrhiza in crops as affected by soil fertility.In Endomycorrhiza. Proc. Symp. Univ. Leeds, July 22–25, 1974. Eds. F A Sanders, B Mosse and P B Tinker. Academic Press, London. pp 495–509.
Howeler R H 1981 Mineral nutrition and fertilization of cassava (Manihot esculenta Crantz). Centro Internacional de Agricultura Tropical, Series 09 EC-4, Cali, Colombia.
Howeler R H, Edwards D G and Asher C J 1979 The effect of soil sterilization and mycorrhizal inoculation on the growth, nutrient uptake and critical P concentration of cassava. Paper presented at Vth Intern. Symp. Trop. Root Crops. Manila, Philippines, Sept. 17–21, 1979 (In press).
Howeler R H, Edwards D G and Asher C J 1981 Application of the flowing solution culture techniques to studies involving mycorrhizas. Plant and Soil 59, 179–183.
Howeler R H, Asher C J and Edwards D G 1982 Establishment of an effective mycorrhizal association on cassava in flowing solution culture and its effect on phosphorus nutrition. New Phytol. 90, 229–238.
IITA Annu. Rep. 1976 International Institute of Trop Agric. Ibadán, Nigeria. pp 80–81.
Instituto Geografico ‘Agustin Codazzi’ 1976 Estudio General de Suelos de los Municipios de Santander de Quilichao, Piendamó, Morales, Buenos Aires, Cajibío y Caldono. Volúmen XII No. 4. 466 p.
Kruckelmann H W 1975 Effects of fertilizers, soils, soil tillage and plant species on the frequency of Endogone chlamydospores and mycorrhizal infection in arable soils.In Endomycorrhiza. Proc. Symp. Univ. Leeds July 22–25, 1974. Eds. F A Sanders, B Mosse and P B Tinker. Academic Press, London pp 511–525.
Mosse B, Powell C LI and Hayman D S 1976 Plant growth responses to vesicular arbuscular mycorrhiza. IX Interactions between VA mycorrhiza, rock phosphates and symbiotic nitrogen fixation. New Phytol. 76, 331–342.
Phillips J M and Hayman D S 1970 Improved procedures for cleaning roots and staining parasitic and vesicular arbuscular mycorrhizal fungi for rapid assessment of infection. Trans. Br. Mycol. Soc. 55, 158–161.
Potty V P 1978 Occurrence of vesicular arbuscular mycorrhiza in certain tuber crops. J. Root Crops 4, 49–50.
Stribley D P, Tinker P B and Rayner J H 1980 Relation of internal phosphorus concentration and plant weight in plants infected by vesicular-arbuscular mycorrhizas. New Phytol. 86, 261–266.
Yost R S and Fox R L 1979 Contribution of mycorrhizae to P nutrition of crops growing on an oxisol. Agron. J. 71, 903–908.
Zaag P van der, Fox R L, Pena R S and Yost R S 1979 Phosphorus nutrition of cassava, including mycorrhizal effects on P, K, S, Zn, and Ca uptake. Field Crops Res. 2, 253–263.
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Howeler, R.H., Cadavid, L.F. & Burckhardt, E. Response of cassava to VA mycorrhizal inoculation and phosphorus application in greenhouse and field experiments. Plant Soil 69, 327–339 (1982). https://doi.org/10.1007/BF02372454
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DOI: https://doi.org/10.1007/BF02372454