Abstract
We present a new computer-controlled, electromechanical system for performing simultaneous extension, inflation, and torsion experiments on cylindrical segments of natural and artificial blood vessels. Specimens are tested while immersed in a temperature-controlled, oxygenated, physiologic solution. Deformations are measured within a central region of the specimen using noncontacting video methods. The associated axial loads, luminal pressures, and torques are measured with standard transducers. Data are collected and stored online, and are used in the feedback control of experimental protocols, which are prescribed using custom interactive software. Finally, we present illustrative data obtained from canine aortas and common carotid arteries.
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References
Bergel, D.H. Mechanics of the arterial wall in health and disease. In: Bauer, R.D.; Busse, R.; eds. The arterial system. Berlin, Germany: Springer-Verlag. 1978.
Canfield, T.R.; Dobrin, P.B. Static elastic properties of blood vessels. In Skalak, R.; Chien, S., eds. Handbook of bioengineering. New York: McGraw-Hill. 1987.
Castaneda-Zuniga, W.R.; Sibley, R.; Amplatz, K. The pathologic basis of angioplasty. Angiol. 35:195–205, 1984.
Consigny, P.M.; Tulenko, T.N.; Nicosia, R.F. Immediate and long-term effects of angioplasty-balloon dilation on normal rabbit iliac artery. Arterioscl. 6:265–276; 1986.
Cox, R.H. Three-dimensional mechanics of arterial segmentsin vitro: Methods. J. Appl. Physiol. 36:381–384; 1974.
Cox, R.H. Regional variation of series elasticity in canine arterial smooth muscle. Am. J. Physiol. 234:H542-H551; 1978.
Cox, R.H. Comparison of arterial wall mechanics using ring and cylindrical segments. Am. J. Physiol. 244:H298-H303; 1983.
Dobrin, P.B. Biaxial anisotropy of dog carotid artery: Estimation of circumferential elastic modulus. J. Biomech. 19:351–358; 1986.
Dobrin, P.B. Mechanisms and prevention of arterial injuries caused by balloon embolectomy. Surg. 106:457–466; 1989.
Downs, J.; Halperin, H.R.; Humphrey, J.D.; Yin, F.C.P. An improved video-based computer tracking system for soft biomaterials testing. IEEE Trans. Biomed. Engr. 37:903–907; 1990.
Fronek, K.; Schmid-Schoenbein, G.; Fung, Y.C. An noncontact method for three-dimensional analysis of vascular elasticityin vivo andin vitro. J. Appl. Physiol. 40:634–637; 1976.
Fronek, K.; Fung, Y.C. Mechanical properties of arteries as a function of topography and age. Biorheol. 17:227–234; 1980.
Fung, Y.C.; Fronek, K.; Patitucci, P. Pseudoelasticity of arteries and the choice of its mathematical expression. Am. J. Physiol. 237:H620-H631; 1979.
Fung, Y.C. Biomechanics: Mechanical properties of living tissues. Berlin, Germany: Springer-Verlag; 1981.
Goldberg, E.M.; Goldberg, M.C.; Chowdhury, L.N.; Gould, S.A. The effects of embolectomy-thrombectomy catheters on vascular architecture. J. Cardiovasc. Surg. 24:74–80; 1983.
Hayashi, K.; Nakamura, T. Material test system for the evaluation of mechanical properties of biomaterials. J. Biomed. Matl. Res. 19:133–144; 1985.
How, T.V.; Clarke, R.M. The elastic properties of a polyurethane arterial prosthesis. J. Biomech. 17:597–608; 1984.
Humphrey, J.D.; Strumpf, R.K.; Yin, F.C.P. A theoretically based experimental approach for identifying vascular constitutive relations. Biorheol. 26:687–702; 1989.
Humphrey, J.D.; Strumpf, R.K.; Yin, F.C.P. Determination of a constitutive relation for passive myocardium: I. The function forms. ASME J. Biomech. Engr. 112:333–339; 1990.
Kas'yanov, V.A.; Rachev, A.I. Deformation of blood vessels upon stretching, internal pressure and torsion. Mech. Comp. Matls. 16:76–80; 1980.
Lee, J.S.; Fraser, W.G.; Fung, Y.C. Comparison of elasticity of an arteryin vivo and in excision. J. Appl. Physiol. 25:799–801; 1968.
Lyon, R.T.; Zarins, C.K.; Lu, C.T.; Yang, C.F.; Glagov, S. Vessel, plaque and lumen morphology after transluminal balloon angioplasty. Arterioscl. 7:306–314; 1987.
Mohan, D.; Melvin, J.W. Failure properties of passive human aortic tissue. I—Uniaxial tension tests. J. Biomech. 15:887–902; 1982.
Mohan, D., Melvin, J.W. Failure properties of passive human aortic tissue. II—Biaxial tension tests. J. Biomech. 16:31–44; 1983.
Nahon, D.; Lee, J.M. A two-dimensional incremental study of the static mechanical properties of vascular grafts. Clin. Matls. 1:177–197; 1986.
Ostergaard, J.R.; Oxlund, H. Collagen type III deficiency in patients with rupture of intracranial saccular aneurysms. J. Neurosurg. 67:690–696; 1987.
Patel, D.J.; Fry, D.L. Longitudinal tethering of arteries in dogs. Circ. Res. 19:1011–1021; 1966.
Patel, D.J.; Janicki, J.S. Static elastic properties of the left coronary circumflex artery and the common carotid artery. Circ. Res. 27:149–158; 1970.
Patel, D.J.; Vaishnav, R.N. Basic hemodynamics and its role in disease processes. Baltimore, MD: University Park Press; 1980.
Quigley, M.R.; Heiferman, K.; Kwaan, H.C.; Vidovich, D.; Nora, P.; Cerullo, L.J. Bursting pressure of experimental aneurysms. J. Neurosurg. 67:288–290; 1987.
Sato, M.; Niimi, H.; Okumura, A.; Handa, H.; Hayashi, K.; Moritake, K. Axial mechanical properties of arterial walls and their anisotropy. Med. Biol. Eng. Comput. 17:170–176; 1979.
Sharma, M.G.; Hollis, T.M. Rheological properties of arteries under normal and experimental hypertension conditions. J. Biomech. 9:293–300; 1976.
Stewart, S.F.C.; Lyman, D.J. Essential characteristics of vascular grafts. In: Sawyer, P.N., ed. Modern vascular grafts. New York: McGraw-Hill; 1987.
Stewart, S.F.; Lyman, D.J. Predicting the compliance of small diameter vascular grafts from uniaxial tensile tests. J. Biomech. 23:629–637; 1990.
Takamizawa, K.; Hayashi, K. Strain energy density function and uniform strain hypothesis for arterial mechanics. J. Biomech. 20:7–17; 1987.
Thubrikar, M.J.; Baker, J.W.; Nolan, S.P. Inhibition of atherosclerosis associated with reduction of arterial intraluminal stress in rabbits. Arterioscl. 8:410–420; 1988.
Vaishnav, R.N. Mathematical characterization of the non-linear rheological behavior of the vascular tissue. Biorheol. 17:219–226; 1980.
Vaishnav, R.N.; Vossoughi, J.; Patel, D.J.; Cothran, L.N.; Coleman, B.R.; Ison-Franklin, E.L. Effect of hypertension on elasticity and geometry of aortic tissue from dogs. ASME J. Biomech. Eng 112:70–74; 1990.
van Loon, P.; Klip, W.; Bradley, E.L. Length-force and volume-pressure relationships in arteries. Biorheol. 14:181–201; 1977.
Vinall, P.E.; Simeone, F.A. Whole mounted pressurizedin vitro model study of cerebral arterial mechanics. Blood Vessels 24:51–62; 1987.
Vito, R.P. The mechanical properties of soft tissues: I. A mechanical system for biaxial testing. J. Biomech. 13:947–950; 1980.
Vito, R.P.; Hickey, J. The mechanical properties of soft tissues: II. The elastic response of arterial segments. J. Biomech. 13:951–957; 1980.
von Maltzahn, W.W.; Warriyar, R.G.; Keitzer, W.F. Experimental measurements of elastic properties of media and adventitia of bovine carotid arteries. J. Biomech. 17:839–847; 1984.
Weizsacker, H.W.; Lambert, H.; Pascale, K. Analysis of the passive mechanical properties of rat carotid arteries. J. Biomech. 16:703–715; 1983.
Yin, F.C.P.; Tompkins, W.R.; Peterson, K.L.; Intaglietta, M. A video dimension analyzer. IEEE Trans. Biomed. Eng. 19:376–381; 1972.
Yin, F.C.P.; Spurgeon, H.A.; Kallman, C.H. Age associated alterations in viscoelastic properties of canine aortic strips. Circ. Res. 53:464–472; 1983.
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This project was funded by a grant from The Whitaker Foundation for which we are most grateful. We also wish to acknowledge Mr. John Toeneboehn, who accomplished much of the original mechanical design as part of a senior project; Mr. William W. Calary, who suggested many clever design changes and who did the machining; and Mr. John Downs, who helped with the software development and hardware installation. Finally, our thanks to Dr. Frank Yin for many insightful comments.
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Humphrey, J.D., Kang, T., Sakarda, P. et al. Computer-aided vascular experimentation: A new electromechanical test system. Ann Biomed Eng 21, 33–43 (1993). https://doi.org/10.1007/BF02368162
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DOI: https://doi.org/10.1007/BF02368162