Summary
The concentration range required for calcium activation of skeletal myofibrillar ATPase activity has previously been attributed to simultaneous binding of two calcium ions to each troponin. We present data representative of the majority of myofibrillar preparations and data with acto subfragment-1 (S-1) whose calcium activation of ATPase activity occurs over a much too narrow range of calcium concentrations to be so explained. S-1 binding significantly broadened the range of Ca2+ concentrations over which activation occurred but not to the extent that is associated with simultaneous binding of 2 calcium ions.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Weber, A. and Herz, R., 1963. J. Biol. Chem. 238: 599–605.
Potter, J. D. and Gergely, J., 1975. J. Biol. Chem. 250: 4628–4633.
Weber, A. and Bremel, R. D., 1971. In: Contractility of Muscle Cells and Related Processes (R. J. Podolsky, ed.) pp. 37–53.
Bremel, R. D. and Weber, A., 1972. Nature (New Biol.) 238: 97–101.
Weber, A. and Murray, J. M., 1973. Physiol. Rev. 53: 612–673.
Weber, A., 1970. In: The Physiology and Biochemistry of Muscle as a Food, 2 E. J. Briskey, R. G. Cassens and B. B. Marsh, eds.) Univ. Wisconsin press pp. 383–394.
Bremel, R. D., Murray, J. M. and Weber, A., 1972. Cold Spring Harbor Symp. Quant. Biol. 37: 267–275.
Chaberek, S. and Martell, A. E., 1959. In: Organic Sequestering Agents. John Wiley, NY.
Taylor, E. W., 1979. Crit. Rev. Biochem. 6: 103–164.
Weber, A., Herz, R. and Reiss, I., 1964. Fed. Proc. 23: 896–900.
Weber, A. and Winicur, S., 1961. J. Biol. Chem. 236: 3198–3202.
Chantler, P. D., Sellers, G. R., Szent Gyorgyi, A. G., in press.
Pemrick, S. and Weber, A., 1976. Biochemistry 15: 5193–5198.
Tawada, Y., Ohara, H., Oai, T. and Tawada, K., 1975. J. Biochem. 78: 65–72.
Murray, J. M., Weber, A. and Wegner, A., in press.
Murray, J. M., Knox, M. K., Trueblood, C. and Weber, A., 1980. FEBS Lett. 114: 169–173.
Eaton, B. L., 1976, Science 192: 1337–1339.
Murray, J. M., Knox, M. K. and Weber, A., in press.
Johnson, J. D., Charlton, S. C. and Potter, J. D., 1979. J. Biol. Chem. 254: 3497–3502.
Trybus, K. M. and Taylor, E. W., 1980. Fed. Proc. 39: 1816.
Potter, J. D. and Gergely, J., 1974. Biochemistry 13: 2697–2703.
Parry, D. A. D. and Squire, J. M., 1973. J. Mol. Biol. 75: 35–55.
Huxley, H. E., 1972, Cold Spring Harbor Symp. Quant. Biol. 37: 361–376.
Haselgrove, J. C., 1972. Cold Spring Harbor Symp. Quant. Biol. 37: 341–352.
Hill, T. L., Eisenberg, E. and Greene, L. E., 1980. Proc. Natl. Acad. Sci. 77: 3186–3190.
Phillips, G. N., Jr., Lattman, E. E., Cummins, P., Lee, K. Y. and Cohen, C., 1979. Nature 278: 413.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Murray, J.M., Weber, A. Cooperativity of the calcium switch of regulated rabbit actomyosin system. Mol Cell Biochem 35, 11–15 (1981). https://doi.org/10.1007/BF02358183
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02358183