Abstract
Using overlapping cosmids representing the vascular endothelial growth factor (VEGF) locus, the VEGF gene was mapped by fluorescence in situ hybridization to chromosome 6p12. This localization permits linkage analysis and the identification of gene interaction in the region, as well as alterations of the VEGF structure or expression in cancer cells with chromosome abnormalities.
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Altschul SF, Gish W, Miller W, Myers EW, Lipman DL (1990) Basic local alignment search tool. J Mol Biol 215:403–410
Berkman RA, Merrill MJ, Reinhold WC, Monacci WT, Saxena A, Clark WC, Robertson JT, Ali IU, Oldfield EH (1993) Expression of the vascular permeability factor/vascular endothelial growth factor gene in central nervous system neoplasms. J Clin Invest 91:153–159
Ferrara N (1993) Vascular endothelial growth factor. Trends Cardiovasc Med 3:244–250
Ferrara N, Keith H, Jakeman L, Leung DW (1992) Molecular and biological properties of the vascular endothelial growth factor family of proteins. Endocr Rev 13:18–32
Fidler IJ, Ellis LM (1994) The implications of angiogenesis for the biology and therapy of cancer metastasis. Cell 79:185–188
Folkman J (1995a) Tumor angiogenesis. In: Mendelson J, Holley P, Liotta L (eds) Molecular basis of cancer. Saunders, Philadelphia, pp 206–232
Folkman J (1995b) Angiogenesis in cancer, vascular, rheumatoid and other disease. Nat Med 1:27–31
Fong GH, Rossant J, Gertsenstein M, Breltman ML (1995) Role of the Flt-1 receptor tyrosine kinase in regulating the assembly of vascular endothelium. Nature 376:66–70
Kim KJ, Li B, Winer J, Armanini M, Gillett N, Phillips HS, Ferrara N. (1993) Inhibition of vascular endothelial growth factorinduced angiogenesis suppresses tumor growth in vivo. Nature 362:841–844
O'Reilly MS, Holmgem L, Shing Y, Chen C, Rosenthal RA, Cao Y, Moses M, Lane WS, Sage EH, Folkman J (1994) Angiogenesis: a circulating endothelial cell inhibitor that suppresses angiogenesis and tumor growth. Cold Spring Harb Symp Quant Biol 59:471–482
Plate KH, Breier G, Widch HA, Risau W (1992) Vascular endothelial growth factor is a potential tumor angiogenesis factor in human gliomas in vivo. Nature 359:845–848
Popescu N, Zimonjic D, Hatch C, Bonner W (1994) Chromosomal mapping of the human histone H2A.Z gene to 4824 by in situ fluorescence hybridization. Genomics 20:333–335
Risau W (1991) Vasculogenesis, angiogenesis and endothelial cell differentiation during embryonic development. In: Feinberg RN, Sherer GK, Auerbach R (eds) The development of the vascular system. (Issues in biomedicine) Karger, Basel, pp 58–68
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
Senger DR, Water L, Brown LF, Nagy JA, Yeo KT, Yeo TK, Berse B, Jackman RW, Dvorak AM, Dvorak HF (1993) Vascular permeability factor (VPF, VEGF) in tumor biology. Cancer Metastasis Rev 12:303–324
Shweiki D, Itin A, Soffer D, Keshet E (1992) Vascular endothelial growth factor induced by hypoxia may mediate hypoxia-initiated angiogenesis. Nature 359:843–845
Stein I, Neeman M, Shweiki D, Itin A, Keshet E (1995) Stabilization of vascular endothelial growth factor mRNA by hypoxia and hypoglycemia and coregulation with other ischemia-induced genes. Mol Cell Biol 15:5363–5368
Tischer E, Mitchell R, Hartman T, Silva M, Gospodarowicz D, Fiddes JC, Abraham JA (1991) The human gene for vascular endothelial growth factor/multiple protein forms are encoded through alternative exon splicing. J Biol Chem 266:11947–11954
Zimonjic DB, Popescu NC, Matsui T, Ito M, Chihara K (1994) Localization of the human cholecystokinin-B/gastrin receptor gene to chromosome I Ip15.4–15.5 by fluorescence in situ hybridization. Cytogenet Cell Genet 65:184–185
Zimonjic DB, Rezanka L, DiPaolo JA, Popescu NC (1995) Refined localization of the erbB-3 proto-oncongene by direct visualization of FISH signals on LUT-inverted and contrast-enhanced digital images of DAPI-banded chromosomes. Cancer Genet Cytogenet 80:100–102
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Wei, M.H., Popescu, N.C., Zimonjic, D.B. et al. Localization of the human vascular endothelial growth factor gene,VEGF, at Chromosome 6p12. Hum Genet 97, 794–797 (1996). https://doi.org/10.1007/BF02346191
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DOI: https://doi.org/10.1007/BF02346191