Abstract
Salt sensitivities of six potato cultivars using six levels of sodium chloride (0.0 to 0.25M) were studied in a greenhouse. Responses of these cultivars were also determined in tissue culture by studying rooting of stem segments, increase in length of cultured roots and inhibition of growth of cell suspension cultures using similar salt concentrations. Responses of cultured stem segments and cell suspensions differed from those expressed by whole plants. A close similarity was observed between the salt stress response of whole plants and of cultured roots. The latter technique may provide a preliminary screening method for assessing salt tolerance in potato genotypes.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- BA:
-
6-benzyladenine
- GA3 :
-
gibberellic acid
- I50 :
-
concentration which inhibits growth 50%
- MS:
-
Murashige and Skoog (1962) medium
- NAA:
-
naphthaleneacetic acid
References
Barlass M & Skene KGM (1981) Relative NaCl tolerances of grapevine cultivars and hybridsin vitro. Z. Pflanzenphysiol. 102: 147–156
Beloualy N & Bouharmont J (1992) NaCl-tolerant plants ofPoncirus trifoliata regenerated from tolerant cell lines. Theor. Appl. Genet. 83: 509–514
Bilski JJ, Nelson DC, Maianu A & Conlon RL (1987) Response of potatoes and related wild species to salinity. Am. Potato J. 64: 431
Bilski JJ, Nelson DC & Conlon RL (1988a) The response of four potato cultivars to chloride salinity, sulfate salinity and calcium in pot experiments. Am. Potato J. 65: 85–90
Bilski JJ, Nelson DC & Conlon RL (1988b) Response of six wild potato species to chloride and sulfate salinity. Am. Potato J. 65: 605–612
Bresler E, McNeal BL & Carter DL (1982) Saline and Sodic Soils: Principles, Dynamics, Modeling. Springer-Verlag, Berlin
Drew MC & Dikumwin E (1985) Sodium exclusion from the shoots by roots ofZea mays (cv LG11) and its breakdown with oxygen deficiency. J. Exptl. Bot. 36: 55–62
Epstein E, Norlyn ID, Rugh DW, Kingsbury RW, Kelly DB & Cunningham GA (1980) Saline culture of crops: a genetic approach. Science 210: 399–404
Greenway H & Munns R (1980) Mechanisms of salt tolerance in nonhalophytes. Ann. Rev. Plant Physiol. 31: 149–190
Hoagland DR & Arnon DI (1938) The water-culture method of growing plants without soil. Univ. Calif. Coll. Agric. Circ. No 347, Berkeley
Jennings DH (1976) The effect of sodium chloride on higher plants. Biol. Rev. 51: 453–486
Kirti PB, Hadi S, Kumar PA & Chopra VL (1991) Production of sodium-chloride-tolerantBrassica juncea plants byin vitro selection at the somatic embryo level. Theor. Appl. Genet. 83: 233–237
LaRosa PC, Singh NK, Hasegawa PM & Bressan RA (1989) Stable NaCl tolerance of tobacco is associated with enhanced accumulation of osmotin. Plant Physiol. 91: 855–861
Mass EV & Hoffman GJ (1977) Crop salt tolerance-current assessment. J. Irrigation Drainage 103: 115–134
McHughen A (1987) Salt tolerance through increased vigor in a flax line (STS-11) selected for salt tolerancein vitro. Theor. Appl. Genet. 74: 727–732
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Nitsch JP & Nitsch C (1969) Haploid plants from pollen grains. Science 163: 85–87
Orton TJ (1980) Comparison of salt tolerance betweenHordeum vulgare andH. jubatum in whole plants and callus cultures. Z. Pflanzenphysiol. 98: 105–118
Ramagopal S (1987) Regulation of protein gene expression in roots during salinity stress. In: Key JL & McIntosh L (Eds) Plant Gene Systems and Their Biology (pp 131–138). Alan R. Liss Inc. New York
Smith MK & McComb JA (1981) Effect of NaCl on the growth of whole plants and their corresponding callus cultures. Aust. J. Plant Physiol. 8: 267–275
Strogonov BP, Komizerko IE & Butenko RG (1968) Culturing of isolated glasswort, sorghum, sweet clover and cabbage tissues for comparative study of their salt tolerance. Fiziol. Rast. 15: 203–208
Sumaryati S, Negrutiu I & Jacobs M (1992) Characterization and regeneration of salt- and water-stress mutants from protoplast culture ofNicotiana plumbaginifolia (Viviani). Theor. Appl. Genet. 83: 613–619
Tal M, Heikin H & Dehan K (1978) Salt tolerance in the wild relatives of the cultivated tomato: responses of callus tissues ofLycopersicon esculentum, L. peruvianum andSolanum pennellii to high salinity. Z. Pflanzenphysiol. 86: 231–240
Trivedi S, Galiba G, Sankhla N & Erdei L (1991) Responses to osmotic and NaCl stress of wheat varieties differing in drought and salt tolerance in callus cultures. Plant Sci. 73: 227–232
Vajrabhaya M, Thanapaisal T & Vajrabhaya T (1989) Development of salt tolerant lines of KDML and LPT rice cultivars through tissue culture. Plant Cell Rep. 8: 411–414
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Naik, P.S., Widholm, J.M. Comparison of tissue culture and whole plant responses to salinity in potato. Plant Cell Tiss Organ Cult 33, 273–280 (1993). https://doi.org/10.1007/BF02319012
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02319012