Summary
The combined utilization of photometric, fluorimetric and polarimetric detection liquid chromatography detectors in series for the identification and quantification of D-tryptophan and L-tryptophan was evaluated. Detection limits of about 1 μg were established with the range of linearity extending to about 100 μg. The relative standard deviation of the D-form and L-form tryptophan were 7.32 and 4.22%, respectively. The amount unknown of tryptophan enantiomers in the different mixtures was determined with an accuracy of 1.58% at the 40 μg injection level.
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References
D. W. Armstrong, S. M. Han, Critical Reviews in Anal. Chem.19, 175 (1988).
J. W. Hubbard, D. Ganes, H. K. Lim, K. K. Midha, Clin. Biochem.19, 107 (1986).
G. M. Anderson, W. C. Purdy, Anal. Chem.51, 283 (1979).
I. Morita, M. Kawamoto, M. Hattori, K. Eguchi, K. Sekiba, H. Yoshida, J. Chromatogr.526, 367 (1990).
M. B. Berardino, F. C. Roingeard, N. K. Fukagawa, J. Nutr. Biochem.1, 220 (1990).
C. P. Bearcroft, M. J. G. Farthing, D. Perret, Biomed. Chromatogr.9, 23 (1995).
K. Uchikura, M. Kirisawa, Anal. Sci.7, 971 (1991).
L. Soltes, B. Sebille, N. Thuaud, Chromatographia38, 761 (1994).
S. C. Jacobson, G. Guichon, Anal. Chem.64, 1496 (1992).
W. Boehme, G. Wagner, U. Oehme, U. Priesnitz, Anal. Chem.54, 709 (1982).
E. S. Yeung, L. E. Steenhoek, S. D. Woodruff, J. C. Kuo, Anal. Chem.52, 1399 (1980).
D. K. Lloyd, D. M. Goodall, H. Scrivener, Anal. Chem.61, 1238 (1989).
A. Navas Díaz, F. Garcia Sanchez, A. Aguilar Gallardo, A. Garcia Pareja, Instrument. Science Technology,24, 47 (1996).
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Sánchez, F.G., Díaz, A.N. & Pareja, A.G. HPLC determination of tryptophan enantiomers with photometric, fluorimetric and diode-laser polarimetric detection. Chromatographia 42, 494–498 (1996). https://doi.org/10.1007/BF02290282
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DOI: https://doi.org/10.1007/BF02290282