Summary
A capillary gas-liquid chromatography method for the analysis of free short chain fatty acids in intestinal samples was developed. Analytical results obtained with intestinal samples are influenced not only by high molecular mass matrix constituents but also by matrix components with a molecular weight smaller than 5000 daltons. To achieve a faithful transfer of SCFA into the chromatographic system it was important to avoid accumulation of involatile deposits on the surface of the inlet liner. Cleaning of the inlet linear lead to a significant weakening of matrix effects. The method described, using an Innowax column, is more effective, particularly with respect to precision and sensitivity, and is clearly superior to packed columns. The capillary is the column of choice for the separation of short fatty acids in samples from the small intestine and stomach.
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Schäfer, K. Analysis of short chain fatty acids from different intestinal samples by capillary gas chromatography. Chromatographia 40, 550–556 (1995). https://doi.org/10.1007/BF02290267
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DOI: https://doi.org/10.1007/BF02290267