Summary
Practical aspects related to the preparation of the fluorescein isothiocyanate (FITC) and dichlorotriazinylaminofluorescein (DTAF) derivatives of amino acids for purposes of quantitative analysis are examined and factors affecting quantification are discussed. It is shown that the labelling reaction for both reagents can be speeded up by operating at 40°C. The difficulties with derivatizing amino acids at detectable concentrations are highlighted. In spite of the high sensitivity of CE-LIF, detection sensitivity in real applications is limited by factors external to the analytical process, such as the label chemistry.
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Lalljie, S.P.D., Sandra, P. Practical and quantitative aspects in the analysis of FITC and DTAF amino acid derivatives by capillary electrophoresis and LIF detection. Chromatographia 40, 519–526 (1995). https://doi.org/10.1007/BF02290262
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DOI: https://doi.org/10.1007/BF02290262