Abstract
Cytokinins from normal and witchesbroom malformed stems of proteas were determined by radioimmunoassay following sample resolution by high-performance liquid chromatography (HPLC). Material from the early stages of shoot malformation had increased cytokinin concentrations which, over time, declined to the concentrations found in normal-growing stems. The cytokinin complement of the malformed structures was different from that of normal stems. The high concentrations of isopentenyladenosine detected appear to be related to the loss of the correlative inhibition of lateral buds and the development of the witchesbroom structures and may result from localized changes in cytokinin biosynthesis and/or metabolism.
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Ali A, Fletcher RA (1970) Hormonal regulation of apical dominance in soyabeans. Can J Bot 48:1989–1994
Balàzs E, Sziràki I (1974) Altered levels of indoleacetic acid and cytokinin in geranium stems infected withCorynebacterium fascians. Acta Phytopathol Acad Sci Hung 9:287–292
Barthe P, Bulard C (1974) Identification d'une cytokinine par chromatographie en phase gazeuse à partir de cultures pures deTaphrina cerasi. Can J Bot 52:1515–1518
Ben-Jaacov J (1986) Protea production in Israel. Acta Hortic 185:101–110
Cutting JGM, Lishman AW, van der Hoven A, Wolstenholme BN (1983) Development of a radioimmunoassay for the cytokinin isopentenyl adenosine. Crop Prod 12:133–135
de Swardt D (1989) Aspekte van die vegetatiewe en reproduktiewe ontwikkeling by geselekteerdeProteaceae cultivars (aspects of the vegetative and reproductive development in selectedProteaceae cultivars). Unpublished M.Sc. Thesis, University of Stellenbosch, South Africa
Dorrington RA (1983) A preliminary investigation of witchesbroom: A disease causing teratomas in protea species. Unpublished B.Sc. (Hons) Thesis, University of Cape Town, South Africa
Greene EM (1980) Cytokinin production by microorganisms. Bot Rev 46:25–74
Hillman JR (1984) Apical dominance. In: Wilkens MB (ed) Advanced plant physiology. Pitman, Marshfield, Massachusetts, pp 127–148
Hofman PJ, Featonby-Smith BC, van Staden (1986) The development of ELISA and RIA for cytokinin estimation and their application to a study of lunar periodicity inEcklonia maxima (Osbeck) papenf. J Plant Physiol 122:455–466
Knox-Davies PS, van Wyk PS, Marasas WFO (1986) Diseases of protea and their control in the south-western Cape. Acta Hortic 185:189–200
Letham DS, Palni LM (1983) The biosynthesis and metabolism of cytokinins. Annu Rev Plant Physiol 34:163–197
Martin GC (1987) Apical dominance. Hort Science 22:824–833
Myburgh AC, Rust D (1971) Witches broom in proteas. Information Bulletin no. 34. FFTRI, Stellenbosch, South Africa
Norberg S-O (1968) Studies in the production of auxins and other growth stimulating substances byExobasidium. Symb Bot Ups 19:1–117
Rourke JP (1980) The proteas of Southern Africa. Tafelberg, Cape Town
Roussaux J (1965) Etude preliminaire des modifications induites chez le pois Express Alaska par leCorynebacterium fascians (Tilford) Dowson. Rev Gen Bot 72:21–53
Rubinstein B, Nagao MA (1976) Lateral bud outgrowth and its control by the apex. Bot Rev 42:82–113
Rust D, Myburgh AC (1976) Heksebesem by proteas (witches broom in proteas). Veld Flora 62:21–22
Sachs T, Thimann KV (1967) The role of auxins and cytokinins in the release of buds from apical dominance. Am J Bot 54:136–144
Sagee O, Moaz A, Mertens R, Goren R, Riov J (1986) Comparison of different enzyme immunoassays for measuring indole-3-acetic acid in citrus tissue. Physiol Plant 68:265–270
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Cutting, J.G.M. Determination of the cytokinin complement in healthy and witchesbroom malformed proteas. J Plant Growth Regul 10, 85–89 (1991). https://doi.org/10.1007/BF02279317
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DOI: https://doi.org/10.1007/BF02279317