Summary
Four Capillary Electroseparation methods are distinguished. All have an ultimate efficiency limited only by axial diffusion and are in principle capable of achieving 106 plates in <1 hour.
The main limitation to performance arises from Ohmic heating of the electrolyte. While forced convection at 10ms−1 is recommended to keep tubes cool, the parabolic temperature profile within the electrolyte limits the tube bore which can be used. A simple limiting expression is derived: (dc/μm)3 (E/kV m−1)3 (c/mol dm−3) <3.3×109.
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Knox, J.H. Thermal effects and band spreading in capillary electro-separation. Chromatographia 26, 329–337 (1988). https://doi.org/10.1007/BF02268176
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DOI: https://doi.org/10.1007/BF02268176