Abstract
In situ localization of short low- or single-copy sequences is still difficult in plants. One solution to this problem could be the use of large yeast artificial chromosomes (YACs) for fluorescencein situ hybridization. Two YACs specific for a single copy marker on the long arm of the NOR-chromosome 2 of tomato (Lycopersicon esculentum) were selected. Both probes hybridized exclusively to this chromosome, although one produced a slightly dispersed hybridization signal. Hybridization of these YACs onto potato chromosomes showed a clear single locus on the homoeologous potato chromosome in both cases.
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References
Ambros PF, Matzke MA, Matzke AJM (1986) Detection of a 17 kb unique sequence (T-DNA) in plant chromosomes byin situ hybridization.Chromosoma 94: 11–18.
Bennett M D, Leitch I J (1995) Nuclear DNA amounts in angiosperms.Annals Bot 76: 113–176.
Burke D T, Carle G F, Olson M V (1987) Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors.Science 236: 806–812.
Carle G F, Olson M V (1985) An electophoretic karyotype for yeast.Proc Natl Acad Sci USA 82: 3756–3760.
Fuchs J, Schubert I (1995) Localization of seed protein genes on metaphase chromosomes ofVicia faba via fluorescencein situ hybridization.Chrom Res 3: 94–100.
Fuchs J, Joos S, Lichter P, Schubert I (1994a) Localization of vicilin genes on field bean chromosome II by fluorescentin situ hybridization.J Heredity 85: 487–488.
Fuchs J, Pich U, Meister A, Schubert I (1994b) Differentiation of field bean heterochromatin byin situ hybridization with a repeatedFok1 sequence.Chrom Res 2: 25–28.
Fuchs J, Houben A, Brandes A, Schubert I (1996) Chromosome ‘painting’ in plants—a feasible technique?Chromosoma 104: 315–320.
Ganal M W, Lapitan N L V, Tanksley S D (1988) A molecular and cytogenetic survey of major repeated DNA sequences in tomato (Lycopersicon esculentum)Mol Gen Genet 213: 262–268.
Green E D, Olson M V (1990) Systematic screening of yeast artificial libraries by the use of the polymerase chain reaction.Proc Natl Acad Sci USA 87: 1213–1217.
Hanson R E, Zwick M S, Choi Set al. (1995) Fluorescentin situ hybridization of a bacterial artificial chromosome.Genome 38: 646–651.
Jiang J, Gill B S (1994) Nonisotopicin situ hybridization and plant genome mapping: the first 10 years.Genome 37: 717–725.
Jiang J, Gill B S, Wang G-L, Ronald P C, Ward D C (1995) Metaphase and interphase fluorescencein situ hybridization mapping of the rice genome with bacterial artificial chromosomes.Proc Natl Acad Sci USA 92: 4487–4491.
Lehfer H, Busch W, Martin R, Herrmann R G (1993) Localization of the B-hordein locus on barley chromosomes using fluorescencein situ hybridization.Chromosoma 102: 428–432.
Leitch I J, Heslop-Harrison J S (1993) Physical mapping of four sites of 5S rDNA sequences and one site of the α-amylase-2 gene in barley (Hordeum vulgare).Genome 36: 517–523.
Martin, G B, Ganal M W, Tanksley S D (1992) Construction of a yeast artificial chromosome library of tomato and identification of cloned segments linked to two disease resistance loci.Mol Gen Genet 233: 25–32.
Pich U, Houben A, Fuchs J, Meister A, Schubert I (1994) Utility of DNA amplified by degenerate oligonucleotide-primed PCR (DOP-PCR) from the total genome and defined chromosomal regions of field bean.Mol Gen Genet 243: 173–177.
Pich U, Meister A, Macas Jet al. (1995) Primedin situ labelling facilitates flow sorting of similar sized chromosomes.Plant J 7: 1039–1044.
Shizuya H, Birren B, Kin U-Jet al. (1992) Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA inEscherichia coli using an F-factor-based vector.Proc Natl Acad Sci USA 89: 8794–8797.
Tanksley S D, Ganal M W, Prince J Pet al. (1992) High density molecular linkage maps of the tomato and potato genomes.Genetics 132: 1141–1160.
Vogelstein B, Gillespie D (1979) Preparation and analytical purification of DNA from agarose.Proc Natl Acad Sci USA 76: 615–619.
Woo S-S, Jiang J, Gill B S, Paterson A H, Wing R A (1994) Construction and characterization of a bacterial artificial chromosome library ofSorghum bicolor.Nucleic Acids Res 22: 4922–4931.
Zamir D, Tanksley SD (1988) Tomato genome is comprised largely of fast-evolving low copy-number sequences.Mol Gen Genet 213: 254–261.
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accepted for publication by J. S. (Pat) Heslop-Harrison
The first two authors have equally contributed to this work and both should be considered as first authors.
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Fuchs, J., Kloos, DU., Ganal, M.W. et al. In situ localization of yeast artificial chromosome sequences on tomato and potato metaphase chromosomes. Chromosome Res 4, 277–281 (1996). https://doi.org/10.1007/BF02263677
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DOI: https://doi.org/10.1007/BF02263677