Abstract
The B chromosomes (Bs) of rye (Secale cereale) have been studied at interphase in terms of their chromatin organization, patterns of nuclear disposition and physical association in plants with two, three, and four Bs. The study was made in the Lindström strain of hexaploid wheat, which carries the rye Bs as an addition line, byin situ hybridization with a B-specific probe and by genomicin situ hybridization (GISH) with rye genomic DNA, enabling whole chromosome painting. Repetive sequences common to the As and Bs of rye allow for visualization of the rye B at interphase in the wheat background. A B-specific probe enables the orientation of two or more Bs to be determined, and the combination of both probes used together gives information on the disposition of the Bs and on their patterns of physical association within the nucleus. The Bs form linear ‘strings’, and the ends of their long arms, which can be detected by the B-specific probe, are usually located within the hemisphere of the nucleus that has the least condensed chromatin. There is dose-dependent association, and even numbers (2B, 4B) have a greater preference for association than odd ones (3B).
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Anamthawat-Jónsson K, Schwarzacher T, Leitch AR, Bennett MD, Heslop-Harrison JS (1990) Discrimination between closely relatedTriticeae species using genomic DNA as a probe.Theor Appl Genet 63: 337–348.
Blunden R, Wilkes TJ, Forster JW et al. (1993) Identification of the E3900 family, a second family of rye B chromosome specific repeated sequences.Genome 36: 706–711.
Coen ES, Carpenter R (1986) Transposable elements inAnthirrinum majus: generations of genetic diversity.Trends Genet 2: 292–296.
Cremer T, Kurz A, Zirbel R et al (1993). Role of chromosome territories in the functional compartmentalization of the cell nucleus.Cold Spring Harb Symp Quant Biol 58: 777–791.
Delgado M, Morais-Cecílio L, Neves N, Jones RN, Viegas W (1995) The influence of B chromosomes on rDNA organisation in rye interphase nuclei.Chrom Res 3: 487–491.
Gotoh K (1924) Uber die chromosomenzahl vonSecale cereale L.Bot Mag Tokyo 38: 135–152.
Heslop-Harrison JS, Bennett MD (1990) Nuclear architecture in plants.Trends Genet 6: 401–405.
Islam-Faridi MN, Mujeeb-Kazi A (1995) Visualization ofSecale cereale. DNA in wheat germ plasm by fluorescentin situ hybridization.Theor Appl Genet 90: 595–600.
Jones RN (1996) B chromosomes in plants. Tansley review.New Phytol (in press).
Jones RN, Puertas MJ (1993) The B-chromosomes of rye (Secale cereale) In: Dhir KK, Sareen TS, eds.Frontiers in Plant Science Research, Delhi, India, Bhagwati Enterprises: Chapter 6, 81–112.
Jones RN, Rees H (1969) An anomalous variation due to B chromosomes in rye.Heredity 24: 265–271.
Jones RN, Rees H (1982)B Chromosomes. London: Academic Press.
Leitch IJ, Leitch AR, Heslop-Harrison JS (1991) Physical mapping of plant DNA sequences by simultaneousin situ hybridization of two differently labelled fluorescent probes.Genome 34: 329–333.
Lindström J (1965) Transfer to wheat of accessory chromosomes from rye.Hereditas 54 149–155.
Sandery MJ, Forster JW, Blunden R, Jones RN (1990) Identification of a family of repeated sequences on the rye B chromosome.Genome 33: 908–913.
Scherthan H, Bähler J, Kohli J (1994) Dynamics of chromosome organization and pairing during meiotic prophase in fission yeast.J Cell Biol 127: 273–285.
Schwarzacher T, Anamthawat-Jónsson K, Harrison GE et al. (1992) Genomicin situ hybridization to identify alien chromosomes and chromosome segments in wheat.Theor Appl Genet 84: 778–786.
Schwarzacher T, Leitch AR, Bennett MD, Heslop Harrison JS (1989)In situ localization of parental genomes in a wide hybrid.Ann Bot 64: 315–324.
Wilkes TM, Francki MG, Langridge P et al (1995) Analysis of rye B-chromosome structure using fluorescencein situ hybridization (FISH).Chrom Res 3: 466–472.
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accepted for publication by J. S. (Pat) Heslop-Harrison
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Morais-Cecílio, L., Delgado, M., Jones, R.N. et al. Painting rye B chromosomes in wheat: interphase chromatin organization, nuclear disposition and association in plants with two, three or four Bs. Chromosome Res 4, 195–200 (1996). https://doi.org/10.1007/BF02254959
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DOI: https://doi.org/10.1007/BF02254959