Abstract
Whole blood serotonin (5-HT) was significantly increased in a drug-free autistic group (n=17) compared to age-and sex-matched normal control (n =20). Blood tryptophan (TRP) values and platelet counts were similar in unmedicated autistics and normal subjects; but whole blood concentrations of TRP were significantly lower, and 5-HT values tended to be lower in the medicated group compared to unmedicated autistics. Highly significant intraclass correlation coefficients and low mean percentage differences were found for repeated measures over a year's period of whole blood 5-HT and the platelet count in the unmedicated but not in the medicated group. Blood TRP values were highly variable over time in both the medicated and drug-free autistic groups.
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Anderson, G. M., Young, J. G., Cohen, D. J., Schlicht, K. R., & Patel, N. (1981). Liquid-chromatographic determination of serotonin adn tryptophan in whole blood and plasma.Clinical Chemistry, 27, 775–776.
Anderson, G. M., Minderaa, R. B., van Benthem, P., Volkmar, F. R., & Cohen, D. J. (1984). Platelet imipramine binding in autistic subjects.Psychiatric Research, 11, 133–141.
Anderson, G. M., Volkmar, F. R., Hoder, E. L., Paul, R., McPhedran, P., Minderaa, R. B., Young, J. G., Hansen, C. R., & Cohen, D. J. (1987). Whole blood serotonin in autistic and normal subjects.Journal of Child Psychiatry and Psychology, 28, 885–900.
Bartko, J. J. (1976). On various intraclass correlation reliability coefficients.Psychological Bulletin, 83, 762–765.
Boullin, D. J., Coleman, M., O'Brien, R. A., & Rimland, B. (1971). Laboratory predictions of infantile autism based on 5-hydroxytryptamine efflux from blood platelets and their correlation with the Rimland E-2 score.Journal of Autism and Childhood Schizophrenia, 1, 63–71.
Campbell, M., Friedman, E., Green, W. H., Small, A. M., & Burdoch, E. I. (1976). Blood platelet monoamine oxidase in schizophrenic children and their families.Neuropsychobiology, 2, 239–246.
Cohen, D. J., Young, J. G., & Roth, J. A. (1977). Platelet monoamine oxidase in early childhood autism.Archives of General Psychiatry, 34, 534–537.
Goldstein, M., Mahanand, P., Lee, J., & Coleman, M. (1976). Dopamine-beta-hydroxylase and endogenous total 5-hydroxy-indole levels in autistic patients and controls. In M. Coleman (Ed.),The autistic syndromes (pp. 57–63). Amsterdam: North Holland.
Hanley, H. G., Stahl, S. M., & Freedman, D. X. (1977). Hyperserotonemia and amine metabolites in autistic and retarded children.Arhives of General Psychiatry, 34, 521–531.
Heeley, A. F., & Roberts, G. E. (1965). Tryptophan metabolism in psychotic children.Developmental Medicine and Child Neurology, 7, 46–49.
Hoshino, Y., Kumashiro, H., & Kaneko, M. (1979). Serum serotonin, free truptophan plasma cyclic AMP levels in autistic children.Fukushima Journal of Medical Science, 26, 79–91.
Hoshino, Y., Yamamoto, T., Kaneko, M., Tachibana, R., Watanabe, M., Ono, Y., & Kumashiro, H. (1984). Blood serotonin and free tryptophan concentration in autistic children.Neuropsychobiology, 11, 22–27.
Jackman, H., Luchins, D., & Meltzer, H. Y. (1983). Platelet serotonin levels in schizophrenia: Relationship to race and psychopathology.Biological Psychiatry, 18, 887–902.
Katsui, T., Okuda, M., Usuda, S., & Koizumi, T. (1986). Kinetics of H-serotonin uptake by platelets in infantile autism and developmental language disorder (including five pairs of twins).Journal of Autism and Developmental Disorders, 16, 69–76.
Lucas, A. R., Warner, K., & Gottlieb, J. S. (1971). Biological studies in childhood schizophrenia: Serotonin uptake by platelets.Biological Psychiatry, 3, 123–128.
Minderaa, R. B., Anderson, G. M., Volkmar, F. R., Akkerhuis, G. W., & Cohen, D. J. (1987). Urine 5-hydroxyindoleacetic acid and whole blood serotonin and tryptophan in autistic and normal subjects.Biological Psychiatry, 22, 933–940.
Partington, M. W., Tu, J. B., & Wong, C. Y. (1973). Blood serotonin levels in severe mental retardation.Developmental Medicine and Child Neurology, 15, 616–627.
Ritvo, E. R. (1977). Biochemical studies of children with the syndromes of autism, childhood schizophrenia and related developmental disabilities: A review.Journal of Child Psychology and Psychiatry, 13, 373–379.
Ritvo, E. R., Yuwiler, A., Geller, E., Ortnitz, E. M., Saeger, K., & Plotkin, S. (1970). Increased blood serotonin and platelets in early infantile autism.Archives of General Psychiatry, 23, 566–572.
Schain, R. J., & Freedman, D. X. (1961). Studies on 5-hydroxyindole metabolism in autistic and other mentally retarded children.Journal of Pediatrics, 58, 315–320.
Shaw, C. R., Lucas, J., & Rabinovitch, R. D. (1959). Metabolic studies in childhood schizophrenia.Archives of General Psychiatry, 1, 366–371.
Stahl, S. M., Wood, D. J., Mefford, I. N., Berger, P. A., & Ciaranello, R. D. (1983). Hyperserotonemia and platelet serotonin uptake and release in schizophrenia and affective disorders.American Journal of Psychiatry, 140, 26–30.
Takahashi, S., Kanai, H., & Miyamoto, Y. (1976). Reassesment of elevated serotonin levels in blood platelets in early infantile autism.Journal of Autism and Childhood Schizophrenia, 6, 317–326.
Wirz-Justice, A., Lichtsteiner, M., & Feer, H. (1977). Diurnal and seasonal variations in human platelet serotonin in man.Journal of Neural Transmission, 41, 7–15.
Wirz-Justice, A., & Puhringer, W. (1978). Seasonal incidence of an altered diurnal rythm of platelet serotonin in unipolar depression.Journal of Neural Transmission, 42, 45–53.
Wirz-Justice, A., & Richter, R. (1979). Seasonality in biochemical determinations: A source of variance and a clue to the temporal incidence of affective illness.Psychiatric Research, 1, 61–69.
Yuwiler, A., Ritvo, E. R., Bald, D., Kipper, D., & Koper, A. (1971). Examination of circadian rhythmicity of blood serotonin and platelets in autistic and non-autistic children.Journal of Autism and Childhood Schizophrenia, 1, 421–435.
Yuwiler, A., Ritvo, E. R., Geller, E., Glousman, R., Schneiderman, G., & Matsumo, D. (1975). Uptake and efflux of serotonin from platelets of autistic and nonautistic children.Journal of Autism and Childhood Schizophrenia, 5, 83–98.
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Child Study Center, Yale University
Child Study Center and Department of Laboratory Medicine, Yale University
We appreciate the collaboration of Dr. Amy Lettick and the Benhaven staff, as well as the help of medical and technical staff of the Yale University Health Services. We thank Karin Schlicht and Carla Hrbek for technical assistance and Inge Putter for editorial assistance, and are grateful for the help of the Children's Clinical Research Center Staff. Supported by NIMH grant MH30929, CCRC grant RR00125, NIH grant MH24393, the William T. Grant Foundation, Mr. Leonard Berger, The Gateposts Foundation, the Solomon R. & Rebecca D. Baker Foundation, and the Sophia Foundation for Medical Research.
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Minderaa, R.B., Anderson, G.M., Volkmar, F.R. et al. Whole blood serotonin and tryptophan in autism: Temporal stability and the effects of medication. J Autism Dev Disord 19, 129–136 (1989). https://doi.org/10.1007/BF02212724
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DOI: https://doi.org/10.1007/BF02212724