Abstract
The ascorbic acid (vitamin C) concentrations in 11 species of microalgae commonly used in mariculture were determined. The species examined were 4 diatoms (Chaetoceros calcitrans (Paulsen) Takano,Chaetoceros gracilis Schütt,Skeletonema costatum (Greville) Cleve,Thalassiosira pseudonana (Hustedt, clone 3H) Hasle and Heimdal); 2 prymnesiophytes (Isochrysis sp. (clone T.ISO) Parke,Pavlova lutheri (Droop) Green); 1 prasinophyte (Tetraselmis suecica (Kylin) Butcher); 2 chlorophytes (Dunaliella tertiolecta Butcher,Nannochloris atomus Butcher); 1 eustigmatophyte (Nannochloropsis oculata (Droop) Green); and 1 cryptophyte (Chroomonas salina (Wislouch) Butcher). Duplicate cultures of each species were grown under defined conditions and analysed during both logarithmic and stationary phase of growth.
Average values for ascorbic acid ranged from 9.4 fg cell−1 (N. oculata, stationary phase) to 700 fg cell−1 (S. costatum, stationary phase). This value was generally related to cell size. Levels of ascorbic acid cell−1 increased during the stationary growth phase forS. costatum andD. tertiolecta and decreased forC. gracilis, T. pseudonana, C. salina andN. oculata. Levels did not change significantly for the remaining species.
Average values for per cent ascorbic acid ranged from 0.11% (T. pseudonana, stationary phase) to 1.62% of dry weight (C. gracilis, logarithmic phase). The per cent ascorbic acid was not related to algal class. Also, the percentage between logarithmic and stationary phase cultures differed for many of the species, but differences were unrelated to algal class.Chaetoceros gracilis, T. pseudonana, N. oculata andIsochrysis sp. (T.ISO) had higher per cent ascorbic acid during the logarithmic phase, whereasD. tertiolecta andN. atomus contained more per cent ascorbic acid during the stationary phase.
Despite the differences in the composition of the different microalgae (0.11–1.62% ascorbic acid), all species would provide a rich source of ascorbic acid for maricultured animals, which can require 0.003–0.02% of the vitamin in their diet.
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References
Aaronson S, DeAngelis B, Frank O, Baker H (1971) Secretions of vitamins and amino acids into the environment byOchromonas danica. J. Phycol. 7: 215–218.
Aaronson S, Dhawale SW, Patni J, DeAngelis B, Frank O, Baker H (1977) The cell content and secretion of water-soluble vitamins in several freshwater algae. Arch. Microbiol. 112: 57–59.
Baker JT (1984) Seaweeds in pharmaceutical studies and applications. Hydrobiolgia 116/117: 29–40.
Bayanova YI, Trubachev IN (1981) Comparative evaluation of the vitamin composition of some unicellular algae and higher plants grown under artificial conditions. Appl. Biochem. Microbiol. 17: 292–298.
Brown MR (1991) The amino-acid and sugar composition of 16 species of microalgae used in mariculture. J. exp. mar. Biol. Ecol. 145: 79–99.
Collier A, Ray S, Wilson WB (1956) Some effects of specific organic compounds on marine organisms. Science 124: 220.
De Roeck-Holtzhauer Y, Quere I, Claire C (1991) Vitamin analysis of five planktonic microalgae and one macroalga. J. appl. Phycol. 3: 259–264.
Desjardins LM, Castell, JD, Kean, JC (1985) Synthesis of dehydroascorbic acid by subadult lobsters (Homarus americanus). Can. J. Fish. aquat. Sci. 42: 370–373.
DiDomenico DA, Iverson RL (1977) Uptake of glycollic acid by a marine bivalve. J. exp. mar. Biol. Ecol. 28: 234–254.
Durve VS, Lovell RT (1982) Vitamin C and disease resistence in channel catfish (Ictalurus punctatus). Can. J. Fish. aquat. Sci. 39: 948–951.
Englard S, Seifter S (1986) The biochemical functions of ascorbic acid. Ann. Rev. Nutr. 6: 365–406.
Enright CT, Newkirk GF, Craigie JS, Castell JD (1986) Evaluation of phytoplankton as diets for juvenileOstrea edulis L. J. exp. mar. Biol. Ecol. 96: 1–13.
Epifanio CE, Valenti CC, Turk CL (1981) A comparison ofPhaeodactylum tricornutum andThalassiosira pseudonana as foods for the oyster,Crassostrea virginica. Aquaculture 23: 347–353.
Goldman JC, Dennett MR (1985) Susceptibility of some marine phytoplankton species to cell breakage during filtration and post filtration rinsing. J. exp. mar. Biol. Ecol. 86: 47–58.
Grün M, Loewus FA (1983) Determination of ascorbic acid in algae by high-performance liquid chromatography on strong cation-exchange resin with electrochemical detection. Analyt. Biochem. 130: 191–198.
Guillard RRL, Ryther JH (1962) Studies on marine planktonic diatoms. I.Cyclotella nana Hustedt, andDetonula confervacea (Cleve) Gran. C Can. J. Microbiol. 8: 229–239.
Halver JE, Ashley LM, Smith RR (1969) Ascorbic acid requirement of coho salmon and rainbow trout. Trans. am. Fish. Soc. 98: 762–771.
Hapatte AM, Poulet SA (1990a) Application of high-performance liquid chromatography to the determination of ascorbic acid in marine plankton. J. Liquid Chromatogr. 13: 357–370.
Hapette AM, Poulet SA (1990b) Variation of vitamin C in some common species of marine plankton. Mar. Ecol. Prog. Ser. 64: 69–79.
Hunter B, Magarelli PC Jr, Lightner DV, Colvin LB (1979) Ascorbic acid-dependent collagen formation in penaeid shrimp. Comp. Biochem. Physiol. 64B: 381–385.
Jeffrey SW (1980) Cultivating uni-cellular marine plants. In CSIRO Division of Fisheries and Oceanography Research Report, 1977–1979, pp. 22–43.
Kanazawa A (1985) Nutrition of penaeid prawns and shrimps. In Taki Y, Primavera JJ, Llobrera JA (eds), Proceedings of the First International Conference on the Culture of Penaeid Prawns/Shrimps, Aquaculture Dept., SEAFDEC, Iloilo City, Philippines, 1984. pp. 123–130.
Langdon CJ, Waldock MJ (1981) The effect of algal and artificial diets on the growth and fatty acid composition ofCrassostrea gigas spat. J. mar. biol. Ass., U.K. 61: 431–448.
Lightner DV, Colvin LB, Brand C, Danald DA (1977) ‘Black Death’, a disease syndrome of penaeid shrimp related to a dietary deficiency of ascorbic acid. Proc. World Maricul. Soc. 8: 611–618.
Lin DS, Ilias AM, Connor WE, Caldwell RS, Cory HT, Daves, GD (1982) Composition and biosynthesis of sterols in selected marine phytoplankton. Lipids 17: 818–824.
Loeblich AR, Smith VE (1968) Chloroplast pigments of the marine dinoflagellateGyrodinium resplendens. Lipids 3: 5–13.
Manahan DT, Stephens GC (1983) The use of high performance liquid chromatography to measure dissolved organic compounds in bivalve aquaculture systems. Aquaculture 32: 339–346.
Millikin MR (1982) Qualitative and quantitative nutrient requirements of fishes: a review. Fish. Bull. U.S. 80: 655–686.
Sandnes K, Braekkan OR (1981) Ascorbic acid and the reproductive cycle of ovaries in cod (Gadus morrhua). Comp. Biochem. Physiol. (A) 70: 545–546.
Sato M, Kondo T, Yashinaka R, Ikeda S (1982) Effect of dietary ascorbic acid levels on collagen formation in rainbow trout. Bull Jap. Soc. sci. Fish. 48: 553–556.
Scott JM (1981). The vitamin B12 requirement of the marine rotifierBrachionus plicatilis. J. mar. biol. Ass., U.K. 61: 983–994.
Shigueno K, Itoh S (1988) Use of Mg-L-ascorbyl-2-phosphate as a vitamin C source in shrimp diets. J. World Aquacult. Soc. 19: 168–174.
Smayda TJ (1978) From phytoplankters to biomass. In Sournia A (ed.), Phytoplankton manual, UNESCO monographs on oceanographic methodology UNESCO, 273–279.
Speek AJ, Schrijver J, Schreurs WHP (1984) Fluorometric determination of total vitamin C in whole blood by high-performance liquid chromatography with pre-column derivatization. J. Chromatogr. 305: 53–60.
Stickney RR, McGeachin RB, Lewis DH, Mars J, Riggs A, Sis RF, Robinson EH, Wurts W (1984) Response ofTilapia aurea to dietary vitamin C. J. World Maricult. Soc. 15: 179–185.
Thompson PA, Harrison PJ, Whyte JNC (1990) Influence of irradiance on the fatty acid composition of phytoplankton. J. Phycol. 26: 278–288.
Tolbert BM (1979) Ascorbic acid metabolism and physiological functions. Int. J. Vit. Nutr. Res., Suppl. 19: 127–142.
Volkman JK, Jeffrey SW, Nichols PD, Rogers GI, Garland CD (1989) Fatty acids and lipid classes of ten species of microalgae used in mariculture. J. exp. mar. Biol. Ecol. 128: 219–240.
Webb KL, Chu FE (1983) Phytoplankton as a food source for bivalve larvae. In Pruder DGet al. (eds), Proc. of the 2nd int. conf. aquaculture nutrition, World Mariculture Society, Spec. Publ. No. 2, Louisiana State University, Louisiana, pp. 272–291.
Wefers H, Sies H (1988) The protection by ascorbate and glutathione against microsomal lipid peroxidation is dependant on vitamin E. Eur. J. Biochem. 174: 353–357.
Whyte JNC (1987) Biochemical composition and energy content of six species of phytoplankton used in mariculture of bivalves. Aquaculture 60: 231–241.
Wikfors GH, Twarog TW, Ukeles R (1984) Influence of chemical composition of algal food sources on growth of juvenile oysters,Crassostrea virginica. Biol. Bull. 167: 251–263.
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Brown, M.R., Miller, K.A. The ascorbic acid content of eleven species of microalgae used in mariculture. J Appl Phycol 4, 205–215 (1992). https://doi.org/10.1007/BF02161206
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DOI: https://doi.org/10.1007/BF02161206