Summary
The aim of this study was to examine if vasodilatory responses to acetylcholine or sodium nitroprusside are altered in subjects with essential hypertension. In patients with essential hypertension (mean BP; 121±4 mmHg,n=14) and age-matched control subjects (mean BP; 88±3 mmHg,n=10), the forearm vasodilatory responses to acetylcholine and sodium nitroprusside were examined. The brachial artery was cannulated with a cannula through which drugs were locally infused. During the drug infusions, forearm blood flow was continuously measured using plethysmography. Basal forearm vascular resistance was higher in patients with essential hypertension than in control subjects (27.5±3.8 vs 13.8±1.7 units,P<0.01). The increases in forearm blood flow or decreases in forearm vascular resistance in response to the infusions of acetylcholine were smaller in patients with essential hypertension than in control subjects (P<0.01). However, the increases in forearm blood flow or decreases in forearm vascular resistance in response to the infusions of sodium nitroprusside were similar for the 2 groups. These results may suggest that the endothelium-dependent vasodilatory response to acetylcholine in the forearm resistance arteries is impaired in patients with essential hypertension.
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Furchgott RF, Zawadzki, JV (1980) The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholine. Nature 285:373–376
Furchgott RF, Cherry PD, Zawadzki JV, Jothianadan D (1984) Endothelial cells as mediators of vasodilation of arteries. J Cardiovasc Pharmacol 6:S336-S343
Griffith TM, Edwards DM, Lewis MJ, Newby AC, Henderson AH (1984) The nature of endothelium-derived vascular relaxant factor. Nature 308:645–647
Cocks TM, Angus JA (1983) Endothelium-dependent relaxation of coronary arteries by noradrenaline and serotonin. Nature 305:627–630
Griffith TM, Edwards DH, Davies RL, Harrison TJ, Evans KT (1987) EDRF coordinates the behavior of vascular resistance vessels. Nature 329:442–445
Griffith TM, Edwards DH, Davies RL, Harrison TJ, Evans KT (1988) Endothelium-derived relaxing factor (EDRF) and resistance vessels in an intact vascular bed: A microangiographic study of the rabbit isolated ear. Br J Pharmacol 93:654–662
Furchgott RF, Carvalho MH, Kahn MT, Matsunaga K (1987) Evidence for endothelium-dependent vasodilatation of resistance vessels by acetylcholine. Blood Vessels 24:145–149
Pohl U, Dezsi L, Simon B, Busse R (1987) Selective inhibition of endothelium-dependent dilation in resistance-sized vessels in vivo. Am J Physiol 253:H234-H239
Bhardwarj R, Moore PK (1988) Endothelium-derived relaxing factor and the effects of acetylcholine and histamine on resistance blood vessels. Br J Pharmacol 95:843–853
Vallance P, Collier J, Moncada S (1989) Effects of endothelium-derived nitric oxide on peripheral arteriolar tone in man. Lancet II:997–1000
Konishi M, Su C (1983) Role of endothelium in dilator responses of spontaneously hypertensive rat arteries. Hypertension 5:881–886
Winquist RJ, Bunting PB, Baskin EP, Wallace AA (1984) Decreased endothelium-dependent relaxation in New Zealand genetic hypertensive rats. J Hypertens 2:541–545
Van de Voorde J, Leusen I (1986) Endothelium-dependent and independent relaxation of aortic rings from hypertensive rats. Am J Physiol 19:H711-H717
Lüscher TF, Vanhoutte PM (1986) Endothelium-dependent contractions to acetylcholine in the aorta of the spontaneously hypertensive rat. Hypertension 8:344–348
Lüscher TF, Raij L, Vanhoutte PM (1987) Endothelium-dependent responses in normotensive and hypertensive Dahl rats. Hypertension 9:157–163
Tesfamariam B, Halpern W (1988) Endothelium-dependent and endothelium-independent vasodilation in resistance arteries from hypertensive rats. Hypertension 11:440–444
Lüscher TF, Romero JC, Vanhoutte PM (1986) Bioassay of endothelium-derived vasoactive substances in the aorta of normotensive and spontaneously hypertensive rats. J Hypertens 4 (Suppl 5):148–150
Van de Voorde J, Leusen I (1984) Endothelium-dependent and independent relaxation effects on aorta preparation of renal hypertensive rats. Arch Int Physiol Biochim 92:P35-P36
Lüscher TF, Vanhoutte PM, Raij L (1987) Antihypertensive therapy normalizes endothelium-dependent relaxations in salt-induced hypertension in the rat. Hypertension 225:932–934
Lockette WG, Otsuka Y, Carretero OA (1986) Endothelium-dependent relaxation in hypertension. Hypertension 8(Suppl II):61–66
De Mey JG, Gray SD (1985) Endothelium-dependent reactivity in resistance vessels. Prog Appl Microcirc 88:181–187
Watt PAC, Thurston H (1989) Endothelium-dependent relaxation in resistance vessels from the spontaneously hypertensive rats. J Hypertens 7:661–666
Linder L, Kiowski W, Bühler FR, Lüscher TF (1990) Indirect evidence for release of endothelium-derived relaxing factor in human forearm circulation in vivo. Blunted response in essential hypertension. Circulation 81:1762–1767
Panza JA, Quyyumi AA, Brush JE (1990) Abnormal endothelium-dependent vascular relaxation in patients with essential hypertension. N Engl J Med 323:22–27
Imaizumi T, Takeshita A, Suzuki S, Yoshida M, Ando S, Nakamura M (1990) Age-independent forearm vasodilatation by acetylcholine and adenosine 5′-triphosphate in humans. Clin Sci 78:89–93
Greenfied ADM, Whitney RJ, Mowbray JF (1963) Methods for the investigation of peripheral blood flow. Br Med Bull 19:101–109
Imaizumi T, Takeshita A, Ashihara T, Nakamura M (1985) The effects of sublingual nitroglycerin on forearm vascular resistance in patients with heart failure and normal subjects. Circulation 72:749–752
Oyama Y, Lawasaki H, Hattori Y, Kanno M (1986) Attenuation of endothelium-dependent relaxation in aorta from diabetic rats. Eur J Pharmacol 132:75–8
Meraji S, Jayakody L, Senaratne MP, Thomson AB, Kappagoda T (1987) Endothelium-dependent relaxation in aorta of BB rat. Diabetes 36:78–81
Tejada IS, Goldstein I, Azadzoi K, Krane RJ, Cohen RA (1989) Impaired neurogenic and endothelium-mediated relaxation of penile smooth muscle from diabetic men with impotence. N Engl J Med 320:1025–1030
Gabbiani G, Elemer G, Guelpa C, Valloton MB, Badonnel MC, Hüttner I (1979) Morphologic and functional changes of the aortic intima during experimental hypertension. Am J Pathol 96:399–422
Limas C, Westrum B, Iwai J, Limas CJ (1982) Aortic morphology in salt-dependent genetic hypertension. Am J Pathol 107:378–394
Yanagisawa M, Kurihara H, Kimura S (1988) A novel potent vasoconstrictor peptide produced by vascular endothelial cells. Nature 332:411–415
Roth RA, Wallace KB (1980) Disposition of biogenic amines and angiotensin I by lungs of spontaneously hypertensive rats. Am J Physiol 239:H736-H761
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Yoshida, M., Imaizumi, T., Ando, Si. et al. Impaired forearm vasodilatation by acetylcholine in patients with hypertension. Heart Vessels 6, 218–223 (1991). https://doi.org/10.1007/BF02125100
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DOI: https://doi.org/10.1007/BF02125100