Abstract
The phospholipid composition of two strains ofBradyrhizobium is reported. In contrast to previous studies [Bunn CR, Elkan GH (1970) Can J Microbiol 17:291–295; and Gerson T, Patel JJ (1975) Appl Microbiol 30:193–198], we determined that phosphatidylglycerol is a major phospholipid within this bacterial genus. Furthermore, neither phosphatidylserine nor phosphatidylinositol was detected within lipid extracts derived from these bacteria. In addition to phosphatidylglycerol, other major phospholipids ofBradyrhizobium were shown to include phosphatidylcholine, phosphatidylethanolamine, and cardiolipin. Possible explanations for the discrepancies between the present study and those of previous investigations are discussed.
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Literature Cited
Bunn CR, Elkan GH (1971) The phospholipid composition ofRhizobium japonicum. Can J Microbiol 17:291–295
Collins MD (1986) Lipid composition ofArthrobacter siderocapsulatus, A. viscosus, A. oxamicetus, A. sialophilus, andAgrobacteriuim pseudotsugae. System Appl Microbiol 8:1–7
Das PK, Basu M, Chatterjee GC (1979) Lipid profile of the strains ofAgrobacterium tumefaciens in relation to agrocin resistance. J Gen Appl Microbiol 25:1–9
de Storani MMC, Rosas SB, Ghittoni NE (1986) Alterations in lipid composition of membranes fromRhizobium meliloti exposed to parathion. Bull Environ Contam Toxicol 36:267–270
Dylan T, Ielpi L, Stanfield S, Kashyap L, Douglas C, Yanofsky M, Nester E, Helinski DR, Ditta G (1986)Rhizobium meliloti genes required for nodule development are related to chromosomal virulence genes inAgrobacterium tumefaciens. Proc Natl Acad Sci USA 83:4403–4407
Elkan GH (1981) The taxonomy of theRhizobiaceae. Int Rev Cytol [Suppl 13]: 1–14
Fischl AS, Carman GM (1983) Phosphatidylinositol biosynthesis inSaccharomyces cerevisiae: purification and properties of microsome-associated phosphatidylinositol synthase. J Bacteriol 154:304–311
Fischl A, Homann MJ, Poole MA, Carman GM (1986) Phosphatidylinositol synthase fromSaccharomyces cerevisiae reconstitution, characterization, and regulation of activity. J Biol Chem 261:3178–3183
Geremia RA, Cavaignac S, Zorreguieta A, Toro N, Olivares J, Ugalde RA (1987) ARhizobium meliloti mutant that forms ineffective pseudonodules in alfalfa produces exopolysaccharide but fails to form beta-(1–2) glucan. J Bacteriol 169:880–884
Gerson T, Patel JJ (1975) Neutral lipids and phospholipids of free-living and bacteroid forms of two strains ofRhizobium infective onLotus pedunculatus. Appl Microbiol 30:193–198
Goldfine H (1982) Lipids of prokaryotes—structure and distribution. In: Razin S, Rottem S (eds) Current topics in membranes and transport, vol. 17. New York: Academic Press, pp 2–44
Halverson LJ, Stacey G (1986) Signal exchange in plant-microbe interactions. Microbiol Rev 50:192–225
Jackson BJ, Kennedy EP (1983) The biosynthesis of membrane-derived oligosaccharides: characterization ofmdoB mutants defective in phosphoglycerol transferase I activity. J Biol Chem 258:2394–2398
Jordan DC (1984)Rhizobiaceae. In: Krieg NR, Holt JG (eds) Bergey's manual of systematic bacteriology, vol. 1. Baltimore: Williams and Wilkins, pp 234–256
Leigh JA, Signer ER, Walker GC (1985) Exopolysaccharide-deficient mutants ofRhizobium meliloti that form ineffective nodules. Proc Natl Acad Sci USA 82:6231–6235
Leigh JA, Reed JW, Hanks JF, Hirsch AM, Walker GC (1987)Rhizobium meliloti mutants that fail to succinylate their calcofluor-binding exopolysaccharide are defective in nodule invasion. Cell 51:579–587
Manasse RJ, Corpe WA (1967) Chemical composition of cell envelopes fromAgrobacterium tumefaciens. Can J Microbiol 13:1591–1603
Miller KJ (1985) Effects of temperature and sodium chloride concentration on the phospholipid and fatty acid compositions of a halotolerantPlanococcus sp. J Bacteriol 162:263–270
Miller KJ, Gore RS, Benesi AJ (1988) Phosphoglycerol substituents present on the cyclic beta-1,2-glucans ofRhizobium meliloti 1021 are derived from phosphatidylglycerol. J Bacteriol 170:4569–4575
Miller KJ, Gore RS, Johnson R, Benesi AJ, Reinhold VN (1990) Cell-associated oligosaccharides ofBradyrhizobium spp. J Bacteriol 172:136–142
Puvanesarajah V, Schell FM, Stacey G, Douglas CJ, and Nester EW (1985) Role for 2-linked-beta-d-glucan in the virulence ofAgrobacterium tumefaciens. J Bacteriol 164:102–106
Thompson EA, Kaufman AE, Johnston NC, Goldfine H (1983) Phospholipids ofRhizobium meliloti andAgrobacterium tumefaciens: lack of effect of Ti plasmid. Lipids 18:602–606
Wilkinson SG (1988) Gram-negative bacteria. In: Ratledge C, Wilkinson SG (eds) Microbial lipids. vol. 1. San Diego: Academic Press, pp 299–488
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Miller, K.J., Shon, B.C., Gore, R.S. et al. The phospholipid composition ofBradyrhizobium spp. Current Microbiology 21, 205–210 (1990). https://doi.org/10.1007/BF02092123
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DOI: https://doi.org/10.1007/BF02092123