Summary
The pathogenic stability ofA. longipes was greatest when the composition of the medium promoted maximum sporulation and minimal mycelial proliferation.
A Whatman No. 17 filter paper disc saturated with an 0.1 % dextrose infusion medium from carrots and potatoes minimised mycelial proliferation, and promoted rapid and extensive spore production in two to four days at 25° C. Approximately 75% of the cultural period on 2% PDA was devoted to mycelial proliferation. The difference in extent of mycelial growth in the filter paper and standard methods was apparently instrumental in eliminating a decline in pathogenicity when using the former method. Weekly mycelial subculturing on 2% PDA caused rapid drop in pathogenicity and a total loss of pathogenicity and sporulative ability between the 62nd and 76th day.
The use of a modified filter paper method for large scale inoculum production for greenhouse and field variety trials is discussed.
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Lloyd, H.L. Pathogenic stability of Alternaria longipes (Ell. & Ev.) Mason subjected to different methods of isolation, storage and inoculum production. Mycopathologia et Mycologia Applicata 38, 33–39 (1969). https://doi.org/10.1007/BF02051673
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DOI: https://doi.org/10.1007/BF02051673