Abstract
Of nine organotin compounds tested towards the marine yeastDebaryomyces hansenii, only triphenyltin chloride (Ph3SnCl) and mono-, di-, and tributyltin chloride induced significant K+ release from cells which was symptomatic of viability loss. The general order of toxicity of the butylated compounds was tributyltin chloride (Bu3SnCl) > monobutyltin chloride (BuSnCl3) ≫ dibutyltin chloride (Bu2SnCl2). The overall toxicity of Ph3SnCl was similar to BuSnCl3. Release of K+ induced by butylated tin compounds or by Ph3SnCl was strongly dependent on the external pH. Maximal toxicity occurred at pH 6.5 for Bu3SnCl, BuSnCl3, and Ph3SnCl, whereas maximal toxicity of Bu2SnCl2 occurred at pH 5.0. Toxicity was decreased above or below these values. The toxicity of BuSnCl3, Bu3SnCl, and Ph3SnCl was reduced at salinity levels approximating to sea water conditions. Prior growth ofD. hansenii in 3% (w/v) NaCl also resulted in reduced sensitivity to Bu3SnCl as evidenced by a decreased rate and extent of K+ efflux. Bu3SnCl-induced Na+ release from cells grown in the absence or presence of 3% (w/v) NaCl was low and similar in both cases. It appeared that the monovalent cation was important in the reduction of Bu3SnCl toxicity since Na2SO4 had a similar protective effect as NaCl while CsCl completely prevented K+ efflux. Thus, the effects of external NaCl were related both to Na+ and to Cl−. These results emphasize that cellular and environmental factors influence the toxic effects of organotins and suggests that these compounds may be more effective antimicrobial agents in some environmental niches than in others.
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References
Aldridge WN (1986) The toxicity and biological properties of organotin compounds. In: Cardarelli NF (ed) Tin as a vital nutrient. CRC Press, Boca Raton, Florida, pp 245–261
Aldridge WN, Brown AW (1988) The biological properties of methyl and ethyl derivatives of tin and lead. In: Craig PJ, Glockling F (eds) The biological alkylation of heavy elements. Royal Society of Chemistry, London, pp 147–163
Aldridge WN, Cremer JE (1957) Organotin dithizone complexes. The colorimetric determination of diethyltin and triethyltin compounds. Analyst 82:37–43
Belde PJM, Kessels BGF, Moelans IM, Borst-Pauwels GWFH (1988) Cd2+ uptake, Cd2+ binding and loss of cell K+ by a Cd-sensitive and a Cd-resistant strain ofSaccharomyces cerevisiae. FEMS Microbiol Lett 49:493–498
Blunden SJ, Hobbs LA, Smith PJ (1984) The environmental chemistry of organotin compounds. In: Bowen HJ (ed) Environmental chemistry. Royal Society of Chemistry, London, pp 49–77
Borst-Pauwels GWFH (1981) Ion transport in yeast. Biochim Biophys Acta 650:88–127
Brinckman FE, Olson GJ, Blair WR, Parks EJ (1988) Implications of molecular speciation and topology of environmental metals: uptake mechanisms and toxicity of organotins. In: Adams WJ, Chapman GA, Landis WG (eds) Aquatic toxicology and hazard assessment, vol 10. American Society of Testing Materials, Philadelphia, pp 219–232
Champ MA, Lowenstein FL (1987) TBT: the dilemma of high-technology antifouling paints. Oceanus 30:68–77
Chau YK, Wong PTS, Kramar O, Bengert GA (1981) Methylation of tin in the aquatic environment. In: Proc 3rd Intl Conf on Heavy Metals in the Environment. CEP Consultants Ltd., Edinburgh, pp 641–644
Cooney JJ (1988) Microbial transformations of tin and tin compounds. J Ind Microbiol 3:195–204
Cooney JJ (1988) Interactions between microorganisms and tin compounds. In: Craig PJ, Glockling F (eds) The biological alkylation of heavy elements. Royal Society of Chemistry, London, pp 92–104
Cooney JJ, Hallas LE, Means JC (1981) Tin and microbes in the Chesapeake Bay, U.S.A. In: Proc 3rd Intl Conf on Heavy Metals in the Environment. CEP Consultants Ltd., Edinburgh, pp 243–245
Cooney JJ, de Rome L, Laurence OS, Gadd GM (1989) Effects of organotin and organolead compounds on yeasts. J Ind Microbiol (in press)
Crowe AJ (1987) Review: organotin compounds in agriculture since 1980. Part 2. Acaricidal, antifeedant, chemosterilant and insecticidal properties. Appl Organomet Chem 1:331–346
Davies A, Smith PJ (1980) Recent advances in organotin chemistry. Adv Inorg Chem Radiochem 23:1–77
Eng G, Tierney EJ, Bellama JM, Brinckman FE (1988) Correlation of molecular total surface area with organotin toxicity for biological and physicochemical applications. Appl Organomet Chem 2:171–175
Gadd GM (1986) Toxicity screening using fungi and yeasts. In: Dutka B, Bitton G (eds) Microbial toxicity testing, vol. 2. CRC Press, Boca Raton, pp 43–77
Gadd GM, Mowll JL (1983) The relationship between cadmium uptake, potassium release and viability inSaccharomyces cerevisiae. FEMS Microbiol Lett 16:45–48
Gadd GM, White C (1989) Heavy metal and radionuclide accumulation and toxicity in fungi and yeasts. In: Poole RK, Gadd GM (eds) Metal-microbe interactions. IRL Press, Oxford, pp. 19–38
Gadd GM, Chudek JA, Foster R, Reed RH (1984) The osmotic responses ofPenicillium ochrochloron: changes in internal solute levels in response to copper and salt stress. J Gen Microbiol 130:1969–1975
Gadd GM, Mowll JL, White C, Newby PJ (1986) Methods for assessment of heavy metal toxicity towards fungi and yeasts. Tox Assess 1:169–185
Gilmour CC, Tuttle JH, Means JC (1987) Anaerobic microbial methylation of inorganic tin in estuarine sediment slurries. Microb Ecol 14:233–242
Guard HE, Cobet AB, Coleman WM (1981) Methylation of trimethyltin compounds by estuarine sediments. Science 213:770–771
Guffanti AA, Clejan S, Falk LH, Hicks DB, Krulwich TA (1987) Isolation and characterization of uncoupler-resistant mutants ofBacillus subtilis. J Bacteriol 169:4469–4478
Hallas LE, Cooney JJ (1981) Tin and tin-resistant microorganisms in Chesapeake Bay. Appl Environ Microbiol 41:466–471
Hallas LE, Cooney JJ (1981) Effects of stannic chloride and organotin compounds on estuarine microorganisms. Dev Ind Microbiol 22:529–535
Hallas LE, Means JC, Cooney JJ (1982) Methylation of tin by estuarine microorganisms. Science 215:1505–1507
Hallas LE, Thayer JS, Cooney JJ (1982) Factors affecting the toxic effect of tin on estuarine microorganisms. Appl Environ Microbiol 44:193–197
Huey C, Brinckman FE, Grime S, Iverson WP (1974) The role of tin in bacterial methylation of mercury. In: De Freitas ASW, Kushner DJ, Laham QM, Qadri SA (eds) Proc Intl Conf on Transport of Persistent Chemicals in Aquatic Ecosystems. National Research Council of Canada, Ottawa, pp II-73–II-78
Kessels BGF, Belde PJM, Borst-Pauwels GWFH (1985) Protection ofSaccharomyces cerevisiae against Cd2+ toxicity by Ca2+. J Gen Microbiol 131:2533–2537
Luitjen JGA (1972) Applications and biological effects of organotin compounds. In: Sawyer AK (ed) Organotin compounds, vol 3. Marcel Dekker, New York, pp 931–974
Maguire RJ (1987) Environmental aspects of tributyltin. Appl Organomet Chem 1:475–498
Maguire RJ, Tkacz RK (1985) Degradation of tri-n-butyltin species in water and sediment from Toronto Harbor. J Agric Food Chem 33:947–953
Mowll JL, Gadd GM (1983) Zinc uptake and toxicity in the yeastsSporobolomyces roseus andSaccharomyces cerevisiae. J Gen Microbiol 129:3421–3425
Norris PR, Kelly DP (1977) Accumulation of cadmium and cobalt bySaccharomyces cerevisiae. J Gen Microbiol 99:317–324
Peña A (1975) Studies on the mechanism of K+ transport in yeast. Arch Biochem Biophys 167:397–409
Pettibone GW, Cooney JJ (1986) Effect of organotins on fecal pollution indicator organisms. Appl Environ Microbiol 52:562–566
Pettibone GW, Cooney JJ (1988) Toxicity of methyltins to microbial populations in estuarine sediments. J Ind Microbiol 2:373–378
Ramos S, Peña P, Valle E, Bergillos L, Parra F, Lazo PS (1985) Coupling of protons and potassium gradients in yeast. In: Kulaev IS, Dawes EA, Tempest DW (eds) Environmental regulation of microbial metabolism. Academic Press, London, pp 351–357
Reed RH, Chudek JA, Foster R, Gadd GM (1987) The osmotic significance of glycerol accumulation in exponentially growing yeasts. Appl Environ Microbiol 53:2119–2123
Thayer JS (1984) Organometallic compounds and living organisms. Academic Press, New York
Theuvenet APR, Kessels BGF, Blankensteijn WM, Borst-Pauwels GWFH (1987) A comparative study of K+-loss from a cadmium-sensitive and a cadmium-resistant strain ofS. cerevisiae. FEMS Microbiol Lett 43:147–153
Thompson JAJ, Sheffer MG, Pierce RC, Chau YK, Cooney JJ, Cullen WR, Maguire JR (1985) Organotin compounds in the aquatic environment. National Research Council of Canada, Ottawa
Waldock MJ, Thain JE, Waite ME (1987) The distribution and potential toxic effects of TBT in UK estuaries during 1986. Appl Organomet Chem 1:287–301
Watanabe Y, Takakuwa M (1987) Change of lipid composition ofZygosaccharomyces rouxii after transfer to high sodium chloride culture medium. J Ferment Technol 65:365–369
White C, Gadd GM (1987) The uptake and cellular distribution of zinc inSaccharomyces cerevisiae. J Gen Microbiol 133:727–737
White C, Gadd GM (1987) Inhibition of H+ efflux and K+ uptake and induction of K+ efflux in yeast by heavy metals. Tox Assess 2:437–447
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Laurence, O.S., Cooney, J.J. & Gadd, G.M. Toxicity of organotins towards the marine yeastDebaryomyces hansenii . Microb Ecol 17, 275–285 (1989). https://doi.org/10.1007/BF02012840
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DOI: https://doi.org/10.1007/BF02012840