Abstract
Experiments in shoot regeneration and virulentAgrobacterium tumefaciens-mediated transformation were used to develop a protocol forRubus transformation. This protocol was then used to produce transformedRubus plants fromin vitro internodes inoculated with anAgrobacterium tumefaciens encoding neomycin phosphotransferase on its disarmed T-DNA. Two transformed plants were selected from 800 inoculations on a medium containing 10 µg ml−1 kanamycin. Results indicated that this level of kanamycin successfully selected against non-transformed cells but did not reduce the number of transformed, kanamycin-resistant, shoots formed. Enzyme assays and Southern blot analysis verified the presence of the β-glucuronidase gene in the plant genome.
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Abbreviations
- BA:
-
benzyladenine
- CAT:
-
chloramphenicol acetyl transferase
- CSR:
-
chlorsulfuron resistance
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- β-GUS:
-
β-glucuronidase
- IAA:
-
indoleacetic acid
- IBA:
-
indolebutyric acid
- NAA:
-
naphthaleneacetic acid
- NPT:
-
neomycin phosphotransferase
- TDZ:
-
thidiazuron
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Hassan, M.A., Swartz, H.J., Inamine, G. et al. Agrobacterium tumefaciens-mediated transformation of severalRubus genotypes and recovery of transformed plants. Plant Cell Tiss Organ Cult 33, 9–17 (1993). https://doi.org/10.1007/BF01997592
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DOI: https://doi.org/10.1007/BF01997592