Abstract
We studied the effects of storage temperature on the stability of dehydrated POPC (1-palmitoyl-2-oleoyl-phosphatidylcholine) mixed with sucrose, trehalose, or a sucrose/raffinose mixture. We used DSC to measure the gel-to-fluid phase transition temperature (T m) of POPC after incubation either below or near the glass transition temperature (T g) of the sugars in the mixture. Glass formation by the sugars around fluid-phase POPC led to the lowering ofT m below that of the fully hydrated lipid. Phospholipid phase behavior did not change during storage belowT g. In some samples stored aboveT g, trehalose crystallized completely; in these samples, theT g of POPC increased to that of the partially dehydrated phospholipid. Melting the crystalline sugar re-established its ability to lower POPC'sT m. We conclude that prevention of complete sugar crystallization was important for stability in the dry state, and that storage belowT g conferred long-term stability to the dehydrated sugar-lipid mixtures.
Article PDF
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
References
J. H. Crowe and J. S. Clegg, eds., Dry Biological Systems, Academic Press, NY 1978.
A. C. Leopold, ed., Membranes, Metabolism, and Dry Organisms, Comstock, Ithaca, NY 1986.
C. W. Vertucci and E. E. Roos, Plant Physiol., 94 (1990) 1019.
G. Bryant and J. Wolfe, Cryo-Lett., 13 (1992) 23.
J. Wolfe and G. Bryant, Physical Principles of Membrane Damage Due to Dehydration and Freezing, in Mechanics of Swelling: From Clays to Living Cells and Tissues, NATO ASI series H., 64 (1991) 205.
L. M. Crowe and J. H. Crowe, Arch. Biochem. Biophys., 217 (1982) 582.
W. J. Gordon-Kamm and P. L. Steponkus, Proc. Natl. Acad. Sci. USA, 81 (1984) 6373.
K. L. Koster, M. S. Webb, G. Bryant and D. V. Lynch, Biochim. Biophys. Acta, 1193 (1994) 143.
L. M. Crowe and J. H. Crowe, Biochim. Biophys. Acta, 946 (1988) 193.
J. H. Crowe, L. M. Crowe, J. F. Carpenter, A. S. Rudolph, C. A. Wistrom, B. J. Spargo and T. J. Anchordoguy, Biochim. Biophys. Acta, 947 (1988) 367.
B. M. Smythe, Aust. J. Chem., 20 (1967) 1097.
M. Caffrey, V. Fonseca and A. C. Leopold, 86 (1988) 754.
K. L. Koster and A. C. Leopold, Plant Physiol., 88 (1988) 829.
W. C. McCrone, L. B. McCrone and J. G. Delly, Polarized Light Microscopy, McCrone Research Institute, Chicago, IL 1987.
L. Finegold, F. Franks and R. H. M. Hatley, J. Chem. Soc., Faraday Trans. I, 85 (1989) 2945.
H. Levine and L. Slade, Crit. Rev. Food Sci. Nutr., 30 (1991) 115.
L. Slade and H. Levine, Adv. Food Nutr. Res., 38 (1995) 103.
J. L. Green and C. A. Angell, J. Phys. Chem., 93 (1989) 2880.
H. Levine and L. Slade, BioPharm, 5 (1992) 36.
J. H. Crowe, L. M. Crowe and D. Chapman, Science, 223 (1984) 701.
Z. H. Chang and J. G. Baust, Cryobiol., 28 (1991) 87.
CRC Handbook of Chemistry and Physics, 61st ed., CRC Press, Inc., Boca Raton, FL 1981.
F. E. Young and F. T. Jones, J. Phys. Colloid Chem., 53 (1949) 1334.
Y. Roos and M. Karel, J. Food Sci., 57 (1992) 775.
F. Franks, Biophysics and Biochemistry at Low Temperatures, Cambridge University Press, Cambridge 1985.
Author information
Authors and Affiliations
Additional information
We thank Karen Sicard for her technical assistance, Dr. Karen Olmstead for her statistical expertise, and Dr. Royce Engstrom for his generous help with polarized light microscopy. We also thank two anonymous reviewers for their helpful comments about the manuscript. Funding for this work was provided by the Cooperative State Research Service, NRI Competitive Grants Program/U.S. Department of Agriculture, under Agreement No. 93-37100-8993 to KLK and by NSF-EPSCoR Grant #OSR-9108773 and the South Dakota Future Fund. CLS was supported by a Grant-in-Aid from Sigma Xi.
Rights and permissions
About this article
Cite this article
Koster, K.L., Sommervold, C.L. & Lei, Y.P. The effect of storage temperature on interactions between dehydrated sugars and phosphatidylcholine. Journal of Thermal Analysis 47, 1581–1596 (1996). https://doi.org/10.1007/BF01992847
Issue Date:
DOI: https://doi.org/10.1007/BF01992847