Abstract
The bioavailability of carcinogenic compounds plays an important role in the process of oncogenesis. Chromium in its hexavalent oxidation state is classified as carcinogen to humans. Therefore we studied the uptake of chromate(VI) into Chinese hamster V79 cells and nuclei isolated after the incubation of the whole cells with chromate. The chromium content of cells and nuclei was determined by atomic absorption spectroscopy. Chromate is taken up in a concentration dependent manner and accumulates to about 30 fold over the extracellular concentration of 0.1 mmol/1. Incubating whole cells with the same concentration results in an intranuclear concentration of up to 6 mmol/1 after 3 h. The kinetic parameters (KM = 0.34 mmol/1, Vmax = 0.12 fmol per cell and minute) are in the same order of magnitude as previously published data. The consequences of the high intracellular and intranuclear concentrations are discussed in terms of the genotoxic effects.
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Brady JF, Ishizaki H (1989) A BASIC program for the estimation of Michaelis-Menten parameters by the direct linear plot. Comput Methods Programs Biomed 28: 271–272
Buttner B, Beyersmann D (1985) Modification of the erythrocyte anion carrier by chromate. Xenobiotica 15: 735
Buttner B, Hebeler M, Beyersmann D (1988) Chromate effects on human erythrocytes — investigations on sulfhydryl groups, cross-linking of membrane proteins and electromechanical properties in the Coulter Counter. Sci Tot Environ 71: 555
Connet P, Wetterhahn KE (1983) Metabolism of the carcinogen chromate by cellular constituents. Struct Bond 54: 93
Debetto P, Lazzarini A, Tomasi A, Beltrame M, Arslan P (1986) Chromate uptake and oxidation state of intracellular chromium in rat thymocytes. Cell Biol Int Rep 10: 214
Debetto P, Arslan P, Antolini M, Luciani S (1988) Uptake of chromate by rat thymocytes and role of glutathion in its cytoplasmic reduction. Xenobiotica 18: 657–664
Denniston ML, Uyeki EM (1987) Distribution and HPLC study of chromium-51 binding sites in CHO cells. J Toxicol Environ Health 21: 375–386
Eisenthal R, Cornish-Bowden A (1974) The direct linear plot. A new graphical procedure for estimating enzyme kinetic parameters. Biochem J 139: 715–720
Hartwig A, Beyersmann D (1987) Enhancement of UV and chromate mutagenesis by nickel ions in the Chinese hamster HGPRT assay. Toxicol Environ Chem 14: 33–42
International Agency for the Research on Cancer (IARC) (1987) Chromium and chromium compounds. IARC monographs Suppl 7: 165–168
Kitagawa S, Seki H, Kametami F, Sakurai H (1982) Uptake of hexavalent chromium by bovine erythrocytes and its interaction with cytoplasmic componends — the role of glutathione. Chem-Biol Interact 40: 265–274
Kortenkamp A, Beyersmann D, O'Brien P (1987) Uptake of chromium(III) complexes by erythrocytes. Toxicol Environ Chem 14: 23
Köster A, Beyersmann D (1985) Chromium binding by calf thymus nuclei and effects on chromatin. Toxicol Environ Chem 10: 307–313
Langård S (1979) The time-related subcellular distribution of chromium in the rat liver cell after intravenous administration of Na2 51CrO4. Biol Trace Elem Res 1: 45–54
Ormos G, Manyai S (1977) Chemical modification of erythrocytes. Effect on the velocity of chromate transport. Acta Biochim Biophys Hung 12: 343–352
Richter-Landsberg C, Green LA, Shelansky ML (1985) Cell surface thy-1-cross-reactive glycoprotein in cultured pc12 cells: modulation by nerve growth factor and association with the cytoskeleton. J Neurosci 5: 468–476
Schnell KF, Gerhard S, Schöppe-Fredenburg A (1977) Kinetic characteristics of the sulfate exchange in human red blood cells and human red blood ghosts. J Membr Biol 30: 319–350
Wiegand HJ, Ottenwälder H, Bolt HM (1985) Fast uptake kineticsin vitro of chromium-51(VI) by red blood cells of man and rat. Arch Toxicol 57: 31–34
Wolf T, Kasemann R, Ottenwälder H (1989) Differing effects of chromium(III) and chromium(VI) on nucleotides and DNA. Arch Toxicol Suppl 13: 48–51
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Sehlmeyer, U., Hechtenberg, S., Klyszcz, H. et al. Accumulation of chromium in Chinese hamster V79-cells and nuclei. Arch Toxicol 64, 506–508 (1990). https://doi.org/10.1007/BF01977636
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DOI: https://doi.org/10.1007/BF01977636