Abstract
One of the early events in murine antigen-induced arthritis is the generation of IL-1 in the inflamed joint. We investigated the role of IL-1 in the acute phase of the arthritic process by selective blockage of IL-1 bioactivity by treatment with neutralizing antibodies. Pretreatment with anti-IL-1 antibodies moderately suppressed joint swelling. The decrease in chondrocyte proteoglycan synthesis seen in the acute phase of arthritis was prevented by treatment with anti-IL-1 antibodies. IL-1 does not appear to be a major contributor to the accelerated breakdown of articular cartilage in this model. The major impact of anti-IL-1 antibodies was the prevention of proteoglycan synthesis inhibition which clearly reduced articular cartilage depletion by maintaining normal proteoglycan synthesis.
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References
A. D. Ogilvie, N. C. Wood, E. Dickens, D. Wojtacha and G. W. Duff,In situ hybridisation. Ann. Rheum. Dis.49, 434–439 (1990).
J. A. Eastgate, J. A. Symons, N. C. Wood, F. M. Grinlinton, F. S. diGiovine and G. W. Duff,Correlation of plasma interleukin 1 levels with disease activity in rheumatoid arthritis. Lancetii, 706–709 (1988).
C. A. Dinarello,Interleukin-1 and interleukin-1 antagonist. Blood77, 1627–1652 (1991).
R. W. Farndale, D. J. Buttle and A. J. Barrett,Improved quantitation of sulfated glycosaminoglycans by use of dimethylmethylene blue. Biochem. Biophys. Acta883, 173–177 (1986).
M. W. M. Kruijsen, W. B. van den Berg, L. B. A. van de Putte and W. J. M. van den Broek,Detection and quantification of experimental joint inflammation in mice by measurements of 99m Tc-pertechnetate uptake. Agents and Actions11, 640–642 (1981).
W. B. van den Berg, M. W. M. Kruijsen and L. B. A. van de Putte,The mouse patella assay. An easy method of quantitating articular cartilage chondrocyte function in vivo and in vitro. Rheumatol. Int.1, 165–169 (1982).
N. C. Wood, J. A. Symons, E. Dickens and G. W. Duff,In situ hybridisation of IL-6 in rheumatoid arhritis. Clin. Exp. Immunol.87, 183–189 (1992).
A. A. J. van de Loo and W. B. van den Berg,Effects of murine recombinant interleukin 1 on synovial joints in mice: Measurements of patellar cartilage metabolism and joint inflammation. Ann. Rheum. Dis.49, 238–245 (1990).
J. Schalkwijk, L. A. B. Joosten, W. B. van den Berg and L. B. A. van de Putte,Chondrocyte nonresponsiveness to insulin-like growth factor 1 in experimental artritis. Arth. Rheum.32, 894–900 (1989).
Further reading
F. A. J. van de Loo, O. J. Arntz, I. G. Otterness and W. B. van den Berg,Protection against cartilage proteoglycan synthesis inhibition by anti-interleukin-1 antibodies in experimental arthritis. J. Rheumatol.19, 348–356 (1992).
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van de Loo, F.A.J., Arntz, O.J., Otterness, I.G. et al. Modulation of cartilage destruction in murine arthritis with anti-IL-1 antibodies. Agents and Actions 39 (Suppl 1), C211–C214 (1993). https://doi.org/10.1007/BF01972769
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DOI: https://doi.org/10.1007/BF01972769