Abstract
The distribution of cardiac output (c.o.) was measured by the radioactive microsphere method in rats at different time intervals after the implantation of carrageenanimpregnated sponges. The amount of blood distributed to the developing granulomata increased from day 5 after sponge implantation to day 7, but showed no further increase at day 10. A similar pattern in blood flow was observed in the skin covering the granulomata. Injection of PGE1 (100 ng) into the sponges led to an increase in blood flow, the magnitude of which became gradually larger between days 5 and 10. A similar, though less marked increase in sensitivity to PGE1 was observed in the skin covering the granulomata, PGE1 causing a significant increase in blood flow to the skin on day 10. These changes in sensitivity to exogenous PGE1 may be due to decreasing levels of endogenous PGE and/or maturation of the newly formed blood vessels in the granulation tissue.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
G.I. Schoefl,Studies on Inflammation. III. Growing Capillaries: Their Structure and Permeability, Virchows Arch. path. Anat.337, 97–141 (1963).
D.B. Cater andT.B. Wallington,Inflammatory Changes in Newly Formed Vessels of Carrageenin-Induced Granulomas after Systemic 5-Hydroxytryptamine, Bradykinin, Kallikrein or Lysolecithin, Br. J. exp. Pathol.49, 74–80 (1968).
J.V. Hurley, B. Edwards andK.N. Ham,The Response of Newly Formed Blood Vessels in Healing Wounds to Histamine and Other Permeability Factors, Pathology2, 133–145 (1970).
W.-C. Chang andS. Tsurufuji,Differences in the Mode of Exudative Reaction Between Early Phase and Late Phase of Carrageenin-Induced Inflammation in Rats, Eur. J. Pharmac.36, 7–14 (1976).
H. Holmström, B. Zederfeldt andJ. Ahonen,Blood Flow Through Granulation Tissue in Bipedicle Tube Flaps, Scand. J. Plast. reconstr. Surg.7, 97–101 (1973).
G.P. Lewis andB.A. Mangham,Changes in Blood Flow and Mediator Content of Rabbit Skin Grafts, Br. J. Pharmac.64, 123–128 (1978).
M.G. Johnston, J.B. Hay andH.Z. Movat,The Modulation of Enhanced Vascular Permeability by Prostaglandins Through Alterations in Blood Flow (Hyperemia), Agents and Actions6, 705–711 (1976).
T.J. Williams andM.J. Peck,Role of Prostaglandin-Mediated Vasodilation in Inflammation, Nature, Lond.270, 530–532 (1977).
P. Vadas andJ.B. Hay,Cutaneous Blood Flow Measurements: A Standardization of the Microsphere Assay for Vasoactive Agents, Agents and Actions8, 504–508 (1978).
W.C. Dick, D.M. Grennan andI.J. Zeitlin,Studies on the Relative Effects of Prostaglandins, Bradykinin, 5-Hydroxytryptamine and Histamine on the Synovial Microcirculation in Dogs, Br. J. Pharmac.56, 313–316 (1976).
S. Moncada andR. Custodio,Prostaglandins and Blood Flow in the Dog's Knee Joint, in:Advances in Prostaglandin and Thromboxane Research, vol. 2 (Eds. B. Samuelsson and R. Paoletti: Raven Press, New York, 1976), p. 825.
L.D. de Leve, M.J. Parnham andP.R. Saxena,Alterations in Prostaglandin E 1 -Induced Blood Flow Changes in Granulation Tissue, Br. J. Pharmac.66 99P-100P (1979).
I.L. Bonta, M.J.P. Adolfs andM.J. Parnham,Cannulated Sponge Implants in Rats for the Study of Time-Dependent Pharmacological Influences on Inflammatory Granulomata, J. Pharmacol. Methods2, 1–11 (1979).
P.R. Saxena,Arteriovenous Shunting and Migraine, Res. clin. Stud. Headache6, 89–102 (1978).
B.M. Johnston andP.R. Saxena,The Effect of Ergotamine on Tissue Blood Flow and the Arteriovenous Shunting of Radioactive Microspheres in the Head, Br. J. Pharmac.63, 541–549 (1978).
E.T. Courtade, T. Tsuda, C.R. Thomas andA.M. Dannenberg,Capillary Density in Developing and Healing Tuberculous Lesions Produced by BCG in Rabbits, Am. J. Pathol.78, 243–260 (1975).
K. Ohuchi, H. Sato andS. Tsurufuji,The Content of Prostaglandin E and Prostaglandin F 2α in the Exudate of Carrageenin Granuloma of Rats, Biochim. Biophys. Acta424, 439–448 (1976).
C.W. Denko,Effect of Prostaglandins in Urate Crystal Inflammation, Pharmacology12, 331–339 (1974).
I.L. Bonta, H. Bult, L.L.M. v.d. Ven andJ. Noordhoek,Essential Fatty Acid Deficiency: A Condition to Discriminate Prostaglandin and Non-Prostaglandin Mediated Components of Inflammation, Agents and Actions6, 154–158 (1976).
I.L. Bonta andM.J. Parnham,Time-Dependent Stimulatory and Inhibitory Effects of Prostaglandin E 1 on Exudative and Tissue Components of Granulomatous Inflammation in Rats, Br. J. Pharmac.65, 465–472 (1979).
M.J. Parnham, I.L. Bonta andM.J.P. Adolfs,Modulation of Granulomatous Inflammation by Prostaglandin E. Involvement of Mononuclear Cells, Agents and Actions, Suppl.4, 267–277 (1979).
J.L. Kalliomäki andM. Haataja,Cutaneous Vascular Response to Prostaglandin E 1 in Rheumatoid Arthritis, Scand. J. Rheumatol.3, 191–194 (1974).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Parnham, M.J., de Leve, L.D. & Saxena, P.R. Development of enhanced blood flow responses to prostaglandin E1 in carrageenan-induced granulation tissue. Agents and Actions 9, 510–515 (1979). https://doi.org/10.1007/BF01968120
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01968120