Conclusion
Structures stained by ZIO technique contain both Zn and Os. The distribution of both elements is similar and the ratio Zn/Os is constant.
By substitution of divalent cation such as Zn in the place of K inChampy's, OsO4−KI, staining fixative mixture,Maillet affords a more constant staining technique where Os and Zn are associated and participate in the formation of some organometallic complex at the level of several histological and cytological structures, such as nervous peripheral structures3, 4.
Résumé
La sonde électronique de Castaing nous a permis de déceler la présence de zinc simultanément à celle de l'osmium au niveau des sites de réduction du fixateur-colorant histologique deChampy-Maillet. L'iode ne fait pas partie du complexe organométallique colorant.
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Literatur
M. Maillet, C.r. Soc. biol., Paris153, 939 (1959).
This note was presented at the Colloque Annuel de la Société Française de Microscopie Electronique, held at Dijon, May 1973.
The financial help of the Belgian F.R.F.C. is gratefully acknowledged.
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Gilloteaux, J., Wautier, J., Laduron, D. et al. Detection of zinc inChampy-Maillet's histological stain by electron probe analysis. Experientia 31, 10–12 (1975). https://doi.org/10.1007/BF01924648
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DOI: https://doi.org/10.1007/BF01924648