Abstract
The tropical marine spongeDysidea herbacea contains large numbers of a symbiotic filamentous cyanobacterium identified on the basis of a detailed ultrastructural study asOscillatoria spongeliae. We report the flow-cytometric separation of the symbiont from the sponge cells, and demonstrate by chemical analyses that a unique group of polychlorinated compounds isolated from the whole sponge tissue is limited to the cyanobacterial filaments, whereas the accompanying sesquiterpenoids are found only in the sponge cells. This is the first demonstration that secondary metabolites ascribed to a sponge are localized in prokaryotic symbiont cells.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Conen, P., Holmes, C. F. B., and Tsukitani, Y., Trends biochem. Sci.15 (1990) 98.
Mann, J., Nature358 (1992) 540.
Vacelet, J., Proc. 4th Int. Coral Reef Symp. (Manila)2 (1981) 713.
Wilkinson, C. R., Symbiosis4 (1987) 135.
Rützler, K., New Perspectives in Sponge Biology, p. 455, Ed. K. Rützler. Smithsonian Institution Press, Washington, D. C. 1990.
Wilkinson, C. R., and Vacelet, J., J. exp. mar. Biol. Ecol.37 (1979) 91.
Wilkinson, C., and Garrone, R., Nutrition in the Lower Metazoa, p. 157. Eds D. C. Smith and Y. Tiffon. Pergamon Press, Oxford 1980.
Bergquist, P. R., Pacific Sci.19 (1965) 123.
Bergquist, P. R., and Tizard, C. A., Micronesica3 (1967) 175.
Bergquist, P. R., N. Z. J. Zool.7 (1980) 443.
Berthold, R. J., Borowitzka, M. A., and Mackay, M. A., Phycologia21 (1982) 327.
Larkum, A. W. D., Cox, G. C., Hiller, R. G., Parry, D. L., and Dibbayawan, T. P., Mar. Biol.95 (1987) 1.
Faulkner, D. J., Nat. Prod. Rep.1 (1984) 551; Nat. Prod. Rep.3 (1986) 1; Nat. Prod. Rep4 (1987) 539; Nat. Prod. Rep.5 (1988) 613; Nat. Prod. Rep.7 (1990) 269.
Charles, C., Braekman, J. C., Daloze, D., Tursch, B., Declercq, J. P., Germain, G., and Van Meerssche, M., Bull. Soc. Chim. Belg.87 (1978) 481.
Kazlauskas, R., Murphy, P. T., and Wells, R. J., Tetrahedron Lett. (1978) 4949.
Bergquist, P. R., and Wells, R. J., Marine Natural Products: Chemical and Biological Perspectives, vol. V, pp. 1–15. Ed. P. J. Scheuer. Academic Press, New York 1983.
Cardellina, J. H. II, Marner, F.-J., and Moore, R. E., J. Am. chem. Soc.101 (1979) 240.
Carmeli, S., Moore, R. E., and Patterson, G. M. L., Tetrahedron47 (1991) 2087.
Hofheinz, W., and Oberhänsli, W. E., Helv. chim. Acta60 (1977) 660.
Stierle, A. C., Cardellina, J. H. II, and Singleton, F. L., Experientia44 (1988) 1021.
Elyakov, G. B., Kuznetsova, T., Mikhailov, V. V., Maltsev, I. I., Voinov, V. G., and Fedoreyev, S. A., Experientia47 (1991) 632.
Carté, B., and Faulkner, D. J., Tetrahedron37 (1981) 2335.
Norton, R. S., Croft, K. D., and Wells, R. J., Tetrahedron37 (1981) 2341.
Moore, R. E., Patterson, G. M. L., and Carmichael, W. W., Memoirs of the California Academy of Sciences, No. 13: Biomedical Importance of Marine Organisms, p. 143. Ed. D. G. Fautin. California Academy of Sciences, San Francisco 1988.
Erickson, K. L., and Wells, R. J., Aust. J. Chem.35 (1982) 31.
De Laszlo, S. E., and Williard, P. G., J. Am. chem. Soc.107 (1985) 199.
Kazlauskas, R., Murphy, P. T., and Wells, R. J., Tetrahedron Lett. (1978) 4945.
Braekman, J. C., Daloze, D., Deneubourg, F., Lippert, E., and Van Sande, J., New J. Chem.14 (1990) 705.
Carmely, S., Gebreyesus, T., Kashman, Y., Skelton, B. W., White, A. H., and Yosief, T., Aust. J. Chem.43 (1990) 1881.
Larkum, A. W. D., Kennedy, I. R., and Muller, W. J., Mar. Biol.98 (1988) 143.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Unson, M.D., Faulkner, D.J. Cyanobacterial symbiont biosynthesis of chlorinated metabolites fromDysidea herbacea (Porifera). Experientia 49, 349–353 (1993). https://doi.org/10.1007/BF01923420
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01923420