Summary
Paraffin sections of optimally fixed tissues often fail to react immunohistochemically. Short (15–30 min) exposure of the sections to 0.1% trypsin solution proves to be a very effective method of ‘unmasking’ the antigens.
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Curran, R.C., Gregory, J. The unmasking of antigens in paraffin sections of tissue by trypsin. Experientia 33, 1400–1401 (1977). https://doi.org/10.1007/BF01920206
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DOI: https://doi.org/10.1007/BF01920206