Abstract
The effect of radiation combined with intraperitoneal administration of Z-100, an immunomodulatory arabinomannan extracted fromMycobacterium tuberculosis, was studied using Meth A fibrosarcoma in BALB/c mice and metastasis of Lewis lung carcinoma, 3LL, in C57BL/6 mice.
In mice bearing Meth A fibrosarcoma, a moderate degree of growth inhibition was observed in the group of single therapy with Z-100 or radiation (10 Gy). When radiation was combined with Z-100, the tumor growth was significantly inhibited. In mice bearing 3LL, slight inhibition of pulmonary metastasis was observed in the group of single therapy, while significant degrees of inhibition of primary tumor growth and pulmonary metastasis were observed in the combination group. This suggests the usefulness of combined use of Z-100 in radiation therapy.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- 3LL:
-
Lewis lung carcinoma
- IL-3:
-
Interleukin-3
- BRM(s):
-
Biological response modifier(s)
- N-CWS:
-
Nocardia-Cell wall skeleton
- CSF:
-
Colony stimulating factor
References
Kobatake H, Suekane T, Murakami Y, Niwa S, Okahira A and Kushida H. Studies on hot water extract ofMycobacterium tuberculosis. I. Structural analyses of polysaccharides. Yakugaku Zasshi 1981; 101: 713–722.
Suzuki I, Okuda M, Matsumura A and Kushida H. Effect of SSM, an extract from human type tubercle bacilli, on the granuropectic activity of the reticuloendotherial system. I. The carbon clearance activity in male mice. Pharmacometrics 1977; 14: 363–367.
Miyamoto R, Ohya O, Hayashi Y, Kushida H, Takahashi M and Nomoto K. Effect of SSM on haematopoietic recovery in X-ray irradiated mice (abstract). Proceedings of the Japanese Cancer Association, the 46th Annual Meeting, 1987; p 386.
Kawamura I, Mitsuyama M, and Nomoto, K. Enhanced protection of cyclophosphamide-treated mice against infection with Pseudomonas aeruginosa after treatment with Z-100, a polysaccharide-rich extract fromMycobacterium tuberculosis Aoyama B. Immunopharm. and Immunotoxicol. 1990; 12(3): 331–343.
Wada J, Sasaki H, Hayashi Y, Kushida H, Takahashi M and Nomoto K. Induction of interleukin 1 activity by an immuno-modulator, SSM, extracted fromMycobacterium tuberculosis human type in mice (abstract). Proceedings of the Japanese Cancer Association, the 46th Annual Meeting, 1987; p 386.
Sasaki H, Schmitt D, Hayashi Y, Pollard RB and Suzuki F. Induction of interleukin 3 and tumor resistance by SSM, a cancer immunotherapeutic agent extracted fromMycobacterium tuberculosis. Cancer Res. 1990; 50: 4032–4037.
Pollard RB, Schmitt D, Sasaki H, Hayashi Y and Suzuki F. Release of a mitogenic factor by Splenic Lyt 1 + T-cells from mice treated with SSM, an immunomodulator extracted from human type tubercle bacilli. Anticancer Res. 1990; 10: 285–290.
Hayashi Y, Ebina T, Suzuki F and Ishida N. Interferon inducing activity of an immunotherapeutic anticancer agent, SSM, prepared fromMycobacterium tuberculosis strain Aoyama B. Microbiol. Immunol., 1981; 25: 305–316.
Sasaki H, Schmitt DA, Kobayashi M, Hayashi Y, Pollard RB and Suzuki F. Prolongation of concomitant antitumor immunity in mice treated with Z-100, an arabinomannan extracted fromMycobacterium tuberculosis. Natural Immun. 1993; 12: 152–167.
Sasaki H, Kobayashi M, Ohya O, Hayashi Y, Matsuda K and Nomoto K. Protective effect of Z-100, an immuno modulator extracted fromMycobacterium tuberculosis, on cecal ligation and puncture-induced sepsis in mice bearing solid tumors. Biotherapy 1993; 7(9): 1366–1370.
Shimizu T, Hayashi Y, Kushida H, Takahashi M, Neda K, Ohtaki Y, Ohtaka T, Nakayoshi H and Nomoto K. Antitumor effect of SSM extracted fromMycobacterium tuberculosis strain Aoyama B and its effective mechanism (abstract). Proceedings of the Japanese Cancer Association, the 44th Annual Meeting, Tokyo, 1985; p 153.
Sasaki H, Schmitt D, Hayashi Y, Pollard RB and Suzuki F. Antitumor mechanisms of Z-100, an immunomodulatory arabinomannan extracted fromMycobacterium tuberculosis. The importance of lymphocytes infiltrated into tumor sites. Nat. Immun. 1993; 12: 104–112.
Moroson H, Nowakowski J and Schechter M. Enhanced lymphocyte-mediated killing of tumor cells after tumor irradiationin vivo. Int. J. Radiat. Biol. 1978; 33: 473–482.
Yamashita T and Mochizuki S. The effect of local tumor irradiation on cell-mediated tumor immunity in tumorbearing mice. Proceedings of the Japanese Cancer Association, the 40th Annual Meeting, 1981; p 114.
Ogawa Y, Maeda T, Hirota S, Yoshida S, Ohara S, Morita M, Hamada F, Inomata T, Morita S, Sawada A and Seguchi H. Combination therapy of radiation and SPG (Schizophyllan) in the treatment of MM46 tumor transplanted in C3H/He mice. J. Jpn. Soc. Cancer Ther. 1986; 21: 87.
Miyaji C, Imanaka K, Gose K, Ichiyanagi A, Imajo Y, Kimura S and Ogawa Y. Combination therapy of radiation and immunomodulators in the treatment of MM46 tumor transplanted in C3H/He mice. Histological and histoenzymic studies usingα-naphtyl acetate esterase staining. J. Jpn. Soc. Cancer Ther. 1983; 18: 2023–2033.
Sone S and Fidler IJ.In situ activation of tumoricidal properties in rat alveolar macrophages by Nocardia rubra cell wall skeleton. Cancer Immunol. Immunother. 1982; 1: 203–209.
Suit HD, Sedlacek R, Wagner M, Oesi L, Silobrcic V and Rothman KJ. Effect ofCorynebacterium parvum on the response to irradiation of a C3H fibrosarcoma. Cancer Res. 1976; 36: 1305–1315.
Yamashita T, Kagawa K, Yamamoto T, Yagi M, Okunaka N, Yoneda K and Tsubura E. Metastatic behavior in mice bearing Lewis lung carcinoma and inhibition of pulmonary metastasis by host modulators. Japan. J. Cancer Chemotherapy 1981; 8: 120–127.
Sone S and Fidler IJ. Activation of rat alveolar macrophages to the tumoricidal stage in the presence of progressively growing pulmonary metastases. Cancer Res. 1981; 41: 2401–2406.
Ohya O, Miyamoto R, Hayashi Y, Kushida H, Takahashi M and Nomoto K. Effect of SSM on haematopoiesis and colony stimulating factor in normal mice (abstract). Proceedings of the Japanese Cancer Association 46th Annual Meeting, 1987; p 386.
Cannistra SA, Vellenga E, Groshek P, Rambaldi A and Griffin JD. Human granulocyte-monocyte colony stimulating factor and interleukin 3 stimulate monocyte cytotoxicity through a tumor necrosis factor-dependent mechanism. Blood 1988; 71: 672–676.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hayashi, Y., Sasaki, H., Emori, Y. et al. The effect of combination therapy of radiation and Z-100, an arabinomannan on tumor growth in mice. Biotherapy 7, 63–69 (1993). https://doi.org/10.1007/BF01878156
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01878156