Abstract
The effect of seawater acclimation and adaptation to various salinities on the energetics of gill and kidney of Atlantic salmon (Salmo salar) was examined. Smolts and non-smolts previously reared in fresh water were exposed to a rapid increase in salinity to 30 ppt. Plasma osmolarity, [Na+], [Cl−], [K+] and [Mg++] increased in both groups but were significantly lower in smolts than non-smolts. Gill Na+, K+-ATPase specific activity, initially higher in smolts, increased in both groups after 18 days in seawater. Kidney Na+, K+-ATPase specific activity was not affected by salinity in either group. Gill and kidney citrate synthase specific activity was not affected by seawater exposure in smolts but decreased in non-smolts. In a second experiment, Atlantic salmon smolts reared in fresh water were acclimated to 0, 10 or 30 ppt seawater for 3 months at a temperature of 13–14°C. Gill Na+, K+-ATPase was positively correlated with salinity, displaying 2.5- and 5-fold higher specific activity at 10 and 30 ppt, respectively, than at 0 ppt. Kidney Na+, K+-ATPase specific activity was not significantly affected by environmental salinity. Citrate synthase and cytochrome c oxidase specific activities in gill were slightly (6–13%) lower at 10 ppt than at 0 and 30 ppt, whereas kidney activities were lowest at 30 ppt. Oxygen consumption of isolated gill filaments was significantly higher when incubated in isosmotic saline and at 30 ppt than at 0 ppt, but was not affected by the prior acclimation salinity. The results indicate that although high salinity induces increased gill Na+, K+-ATPase activity, it does not induce substantial increases in metabolic capacity of gill or kidney.
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References
Butler, D.G., and Carmichael, F.J. 1972. (Na+−K+)-ATPase activity in eel (Anguilla rostrata) gills in relation to change in environmental salinity: role of adrenocortical steroids. Gen. Comp. Endocrinol. 19: 421–427.
Chretien, M. and Pisam, M. 1986. Cell renewal, and differentiation in the gill epithelium of fresh-or salt-water-adapted euryhaline fish as revealed by [3H]-thymidine radioautography. Biol. Cell. 56: 137–150
Conte, F.P. 1969. The biochemical aspects of salt secretion.In Fish in Research. pp. 105–120. Edited by O.W. Neuhaus and J.E. Halver. Academic Press, New York.
Dange, A.D. 1985. Branchial Na+−K+-ATPase activity during osmotic adjustments in two freshwater euryhaline teleosts, tilapia (Sarotherodon mossambicus) and organe chromid (Etroplus maculatus). Mar. Biol. 87: 101–107.
Doneen, B.A. 1981. Effects of adaptation to sea water, 170% sea water and to fresh water on activities and subcellular distribution of branchial Na+, K+-ATPase, low- and high affinity Ca++-ATPase, and oubain-insensitive ATPase inGillichthys mirabilis. J. Comp. Physiol. 145: 51–61.
Eddy, F.B. 1975. The effect of calcium on gill potentials and on sodium and chloride fluxes in the goldfishCarassius auratus. J. Comp. Physiol. 96: 131–142.
Eddy, F.B. 1982. Osmotic and ionic regulation in captive fish with particular reference to salmonids. Comp. Biochem. Physiol. 73B: 125–141.
Epstein, F.H., Katz, A.I. and Pickford, G.E. 1967. Sodium- and potassium-activated adenosinetriphosphatase of gills: role in adaptation to salt water. Science 156: 1245–1247.
Evans, D.H. 1979. Fish.In: Osmotic and Ionic Regulation in Animals, Vol 1. pp. 305–390. Edited by G.M.O. Maloiy. Academic Press, London.
Evans, D.H. 1984. The role of gill permeability and transport mechanisms in euryhalinity.In Fish Physiology, Vol. XB. pp. 239–283. Edited by W.S. Hoar and D.J. Randall. Academic Press, New York.
Farmer, G.J. and Beamish, F.W.H. 1969. Oxygen consumption ofTilapia nilotica in relation to swimming speed and salinity. J. Fish. Res. Bd. Can 26: 2807–2821.
Febry, R., and Lutz, P. 1987. Energy partitioning in fish: the activity-related cost of osmoregulation in a euryhaline cichlid. J. Exp. Biol. 128: 63–85.
Forrest, J.N., Jr., Cohen, A.D., Schon, D.A. and Epstein, F.H. 1973. Na transport and Na−K-ATPase in gills during adaptation to sea water: effects of cortisol. Am. J. Physiol. 224: 709–713.
Foskett, J.K. and Scheffey, C. 1982. The chloride cell: definitive identification as the salt-secretory cell in teleosts. Science 215: 164–166.
Hoar, W.S. 1988. The physiology of smolting salmonids.In Fish Physiology, Vol. XIB, pp. 275–343 Edited by W.S. Hoar and D.J. Randall Academic Press, New York.
Hochachka, P.W. and Somero, G.N. 1984. Biochemical Adaptation. Princeton University Press, Princeton.
Jampol, L.M. and Epstein, F.H. 1970. Sodium-potassium-activated adenosine triphosphatase and osmotic regulation by fishes. Am. J. Physiol. 218: 607–611.
Jurss, K., Bittorf, T. and Vokler, T. 1984. Biochemical investigations on salinity and temperature acclimation of the rainbow trout,Salmo gairdneri (Richardson) Zool. Jb. Physiol. 88: 67–81.
Jurss, K., Bittorf, T. and Vokler, T. 1985. Influence of salinity and ratio of lipid to protein in diets on certain enzyme activities in rainbow trout (Salmo gairdneri Richardson) Comp. Biochem. Physiol. 81B: 73–79.
Itazawa, Y. and Oikawa, S. 1983. Metabolic rates in excised tissues of carp. Experientia 39: 160–161.
Kirschner, L.B. 1980. Comparison of vertebrate salt-excreting organs. Am. J. Physiol. 238: R219-R223.
Langdon, J.S. 1987. Active osmoregulation in the Australian Bass,Macquaria novemaculeata (Steindachner), and the golden perch,Macquaria ambigua (Richardson) (Percichthydae). Aust. J. Mar. Freshw. Res. 38: 771–776.
Langdon, J.S. and Thorpe, J.E. 1984. Response of the gill Na+, K+-ATPase activity, succinic dehydrogenase activity and chloride cells to saltwater adaptation in Atlantic salmon,Salmo salar L., parr and smolt. J. Fish Biol. 24: 323–331.
Lassere, P. 1978. Increase of (Na++K+) dependent ATPase activity in gills and kidneys of two euryhaline marine teleosts,Crenimugil labrosus (Risso, 1826) andDicentrarchus labrax (Linnaeus, 1758), during adaptation to fresh water. Life Sci. 10: 113–119.
Leray, C., Colin, D.A. and Florentz, A. 1981 Time course of osmotic adaptation and gill energetics of rainbow trout (Salmo gairdneri R.) following abrupt changes in salinity. J. Comp. Physiol. 144: 175–181.
Mandel, L.J. 1986. Bioenergetics of membrane transport processes.In Physiology of Membrane Disorders. pp. 295–310. Edited by T.E. Andreoli, J.F. Hoffman, D.D. Fanestil and S.G. Schultz. Plenum Medical Book Co., New York.
McCormick, S.D. and Saunders, R.L. 1987. Preparatory physiological adaptations for marine life of salmonids: osmoregulation, growth, and metabolism. Am. Fish. Soc. Symp. 1: 211–229.
McCormick, S.D. and Bern, H.A. 1989.In vitro stimulation of Na+, K+,-ATPase activity and ovarian binding by cortisol in coho salmon gill. Am. J. Physiol. (In press).
McCormick, S.D., Saunders, R.L., Henderson, E.B. and Harmon, P.R. 1987. Photoperiod control of parr-smolt transformation in Atlantic salmon (Salmo salar): changes in salinity tolerance, gill Na+, K+-ATPase activity, and plasma thyroid hormones. Can. J. Fish. Aq. Sci. 44: 1462–1468.
McCormick, S.D., Saunders, R.L. and MacIntyre, A.D. 1989. Mitochondrial enzyme and Na+, K+-ATPase activity, and ion regulation during parr-smolt transformation in Atlantic salmon (Salmo salar). Fish Physiol. Biochem. This issue, pp 231–241.
Perry, S.F., Lauren, D.J. and Booth, C.E. 1984. Absence of branchial edema in perfused heads of rainbow trout (Salmo gairdneri). J. Exp Zool. 231: 441–445.
Potts, W.T.W., Fletcher, C.R. and Eddy, F.B. 1973. An analysis of the sodium and chloride fluxes in the flounderPlatichthys flesus. J. Comp. Physiol. 87: 21–28.
Rao, G.M. 1968. Oxygen consumption of rainbow trout (Salmo gairdneri) in relation to activity and salinity. Can. J. Zool. 46: 781–786.
Sargent, J.R., Thomson, A.J. and Bornancin, M. 1975. Activities and localization of succinic dehydrogenase and Na+/K+-activated adenosine triphosphatase in the gills of freshwater and seawater eels (Anguilla anguilla). Comp. Biochem. Physiol. 51B: 75–79.
Saunders, R.L., Henderson, E.B. and Harmon, P.R. 1985. Effects of photoperiod on juvenile growth and smolting of Atlantic salmon and subsequent survival and growth in sea cages. Aquaculture 45: 55–66.
Sidell, B.D. 1983. Cellular acclimatization to environmental change by quantitative alterations in enzymes and organelles.In Cellular Acclimatization to Environmental Change pp. 103–120. Edited by A.R. Cossins and P. Sherterline. Cambridge Univ. Press, Cambridge.
Stagg, R.M. and Shuttleworth, T.J. 1982. Na+, K+-ATPase, ouabain binding and oubain-sensitive oxygen consumption in gills fromPlatichthys flesus adapted to seawater and fresh water. J. Comp. Physiol. 147: 93–99.
Stuenkel, E.L. and Hillyard, S.D. 1980. Effects of temperature and salinity on gill Na+, K+-ATPase activity in the pupfish,Cyprinodon salinus. Comp. Biochem. Physiol. 67A: 179–182.
Towle, D.W. 1981. Role of Na++K+-ATPase in ionic regulation by marine and estuarine animals. Mar. Biol. Letters 2: 107–122.
Towle, D.W., Gilman, M.E. and Hempel, J.D. 1977. Rapid modulation of gill Na++K+-dependent ATPase activity during acclimation of the killifishFundulus heteroclitus to salinity change. J. Exp. Zool. 202: 179–186.
Utida, S., Kamiya, M. and Shirai, N. 1971. Relationship between the activity of Na+,K+-activated adenosinetriphophatase and the number of chloride cells in eel gills with special reference to sea-water adaptation. Comp. Biochem. Physiol. 38A: 443–447.
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McCormick, S.D., Moyes, C.D. & Ballantyne, J.S. Influence of salinity on the energetics of gill and kidney of Atlantic salmon (Salmo salar). Fish Physiol Biochem 6, 243–254 (1989). https://doi.org/10.1007/BF01875027
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DOI: https://doi.org/10.1007/BF01875027