Summary
The hydraulic resistance was measured on internodal cells ofNitellopsis obtusa using the method of transcellular osmosis. The hydraulic resistance was approximately 2.65 pm−1 sec Pa, which corresponds to an osmotic permeability of 101.75 μm sec−1 (at 20°C).p-Chloromercuriphenyl sulfonic acid (pCMPS) (0.1–1mm, 60 min) reversibly increases the hydraulic resistance in a concentration-dependent manner.pCMPS does not have any effect on the cellular osmotic pressure.pCMPS increases the activation energy of water movement from 16.84 to 32.64 kJ mol−1, indicating that it inhibits water movement by modifying a low resistance pathway.pCMPS specifically increases the hydraulic resistance to exosmosis, but does not influence endosmosis. By contrast, nonyltriethylammonium (C9), a blocking agent of K+ channels, increases the hydraulic resistance to endosmosis, but does not affect that to exosmosis. These data support the hypothesis that water moves through membrane proteins in characean internodal cells and further that the polarity of water movement may be a consequence of the differential gating of membrane proteins on the endo- and exoosmotic ends.
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Benga, G. 1989. Water exchange through the erythrocyte membrane.Int. Rev. Cytol. 114:273–316
Benga, G., Brain, A., Pop, V.I., Hodarnau, A., Wigglesworth, J.M. 1987. Freeze-fracture electron microscopic observations on the effects of sulphydryl group reagents on human erythrocyte membranes.Cell Biol. Int. Rep. 11:679–687
Benga, G., Pop, V.I., Popescu, O., Ionescu, M., Mihele, M. 1983. Water exchange through erythrocyte membranes: Nuclear magnetic resonance studies on the effects of inhibitors and of chemical modification of human membranes.J. Membrane Biol. 76:129–137
Benga, G., Popescu, O., Borza, V., Pop, V.I., Hodarnau, A. 1989. Water exchange through erythrocyte membranes: Biochemical and nuclear magnetic resonance studies re-evaluating the effects of sulfhydryl reagents and of proteolytic enzymes of human membranes.J. Membrane Biol. 108:105–113
Benz, R., Tosteson, M.T., Schubert, D. 1984. Formation and properties of tetramers of band 3 protein from human erythrocyte membranes in planer lipid bilayers.Biochim. Biophys. Acta 775:347–355
Boyer, J.S., Wu, G. 1978. Auxin increases the hydraulic conductivity of auxin-sensitive hypocotyl tissue.Planta 139:227–237
Brown, P.A., Feinstein, M.B., Sha'afi, R.I. 1975. Membrane proteins related to water transport in human erythrocytes.Nature (London) 254:523–525
Carceller, M.S., Sánchez, R.A. 1972. The influence of phytochrome on the water exchange of epidermal cells ofTaraxacum ofticinale.Experientia 28:364
Dainty, J., Ginzburg, B.Z. 1964a. The measurement of hydraulic conductivity (osmotic permeability to water) of internodal characean cells by means of transcellular osmosis.Biochim. Biophys. Acta 79:102–111
Dainty, J., Ginzburg, B.Z. 1964b. The permeability of the cell membrane ofNitella translucens to urea, and the effect of high concentrations of sucrose on this permeability.Biochim. Biophys. Acta 79:112–121
Dick, D.A.T. 1966. Cell Water. Butterworths, London
Dowler, M.J., Rayle, D.L., Cande, W.Z., Ray, P.M., Durand, H., Zenk, M.H. 1974. Auxin does not alter the permeability of pea segments to tritium-labeled water.Plant Physiol. 53:229–232
Finklestein, A. 1987. Water movement through lipid bilayers, pores, and plasma membranes. Theory and reality. Distinguished Lecture Series of the Society of General Physiologists. Vol. 4. John Wiley & Sons, New York-Chichester-Brisbane-Toronto-Singapore
Fischbarg, J., Liebovitch, L.S., Koniarek, J.P. 1987. Inhibition of transepithelial osmotic water flow by blockers of the glucose transporter.Biochim. Biophys. Acta 898:266–274
Gutknecht, J. 1967. Membranes ofValonia ventricosa: Apparent absence of water-filled pores.Science 158:787–788
Gutknecht, J. 1968. Permeability ofValonia to water and solutes: Apparent absence of aqueous membrane pores.Biochim. Biophys. Acta 163:20–29
Hansson Mild, K., Løvtrup, S. 1985. Movement and structure of water in animal cells. Ideas and experiments.Biochim. Biophys. Acta 822:155–167
Kamiya, N., Kuroda, K. 1956. Artificial modification of the osmotic pressure of the plant cell.Protoplasma 46:423–436
Kamiya, N., Tazawa, M. 1956. Studies on water permeability of a single plant cell by means of transcellular osmosis.Protoplasma 46:394–422
Kang, B.G., Burg, S.P. 1971. Rapid change in water flux induced by auxins.Proc. Natl. Acad. Sci. USA 68:1730–1733
Kiyosawa, K. 1975. Studies on the effects of alcohols on membrane water permeability onNitella.Protoplasma 86:243–252
Kiyosawa, K., Ogata, K. 1987. Influence of external osmotic pressure on water permeability and electrical conductance ofChara cell membrane.Plant Cell Physiol. 28:1013–1022
Kiyosawa, K., Tazawa, M. 1972. Influence of intracellular and extracellular tonicities on water permeability in characean cells.Protoplasma 74:257–270
Kiyosawa, K., Tazawa, M. 1973. Rectification characteristics ofNitella membranes in respect to water permeability.Proctoplasma 78:203–214
Kohn, P.G. 1965. Tables of some physical and chemical properties of water.Symp. Soc. Exp. Biol. 19:3–16
Kukita, F., Yamagishi, S. 1983. Effects of an outward water flow on potassium currents in a squid giant axon.J. Membrane Biol. 75:33–44
Lichtner, F.T., Lucas, W.J., Spanswick, R.M. 1981. Effect of sulfhydryl reagents on the biophysical properties of the plasmalemma ofChara corallina.Plant Physiol. 68:899–904
Loros, J., Taiz, L. 1982. Auxin increases the water permeability ofRhoeo andAllium epidermal cells.Plant Sci. Lett. 26:93–102
Lucas, W.J., Alexander, J.M. 1980. Sulfhydryl group involvement in plasmalemma transport of HCO −3 and OH− inChara corallina.Plant Physiol. 65:274–280
Lukacovic, M.F., Toon, M., Solomon, A.K. 1984. Site of cation leak induced by mercurial sulfhydryl reagents.Biochim. Biophys. Acta 772:313–320
Naccache, P., Sha'afi, R.I. 1974. Effect of PCMBS on water transfer across biological membranes.J. Cell Physiol. 83:449–456
Pike, C.S. 1976. Lack of influence of phytochrome on membrane permeability to tritiated water.Plant Physiol. 57:185–187
Shiina, T., Tazawa, M. 1987a. Ca2+-activated Cl− channel in plasmalemma ofNitellopsis obtusa.J. Membrane Biol. 99:137–146
Shiina, T., Tazawa, M. 1987b. Demonstration and characterization of Ca2+-channel in tonoplast-free cells ofNitellopsis obtusa.J. Membrane Biol. 96:263–276
Steudle, E., Tyerman, S.D. 1983. Determination of permeability coefficients, reflection coefficients, and hydraulic conductivity ofChara corallina using the pressure probe: Effects of solute concentration.J. Membrane Biol. 75:85–96
Steudle, E., Zimmermann, U. 1974. Determination of the hydraulic conductivity and of reflection coefficients inNitella flexilis by means of direct cell-turgor pressure measurement.Biochim. Biophys. Acta 332:399–412
Tazawa, M., Kamiya, N. 1965. Water relations of characean internodal cell.Annu. Rep. Biol. Works (Fac. Sci., Osaka Univ.) 13:123–419
Tazawa, M., Kamiya, N. 1966. Water permeability of a characean internodal cell with special reference to its polarity.Aust. J. Biol. Sci. 19:399–419
Tazawa, N., Shimmen, T. 1980. Demonstration of the K+ channel in the plasmalemma of tonoplast-free cells ofChara australis.Plant Cell Physiol. 21:1535–1540
Tsutsui, I., Ohkawa, T., Nagai, R., Kishimoto, U. 1987. Role of calcium ion in the excitability and electrogenic pump activity of theChara corallina membrane: II. Effects of La3+, EGTA, and calmodulin antagonists on the current voltage relation.J. Membrane Biol. 96:75–84
Tyerman, S.D., Findlay, G.P., Paterson, G.J. 1986. Inward membrane current inChara inflata: II. Effects of pH, Cl−-channel blockers and NH +4 , and significance for the hyperpolarized state.J. Membrane Biol. 89:153–161
Wayne, R., Tazawa, M. 1988. The actin cytoskelton and polar water permeability in characean cells.Protoplasma Suppl.2: 116–130
Weisenseel, M.H., Smeibidl, E. 1973. Phytochrome controls the water permeability inMougeotia.Z. Pflanzenphysiol. 70:420–431
Whittembury, G., Carpi-Medina, P., Gonzalez, E., Linares, H. 1984. Effect ofpara-chloromercuribenzenesulfonic acid and temperature on cell water osmotic permeability of proximal straight tubules.Biochim. Biophys. Acta 775:365–373
Will, P.C., Hopfer, U. 1979. Apparent inhibition of active nonelectrolyte transport by an increased sodium permeability of the plasma membrane.J. Biol. Chem. 254:3806–3811
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Wayne, R., Tazawa, M. Nature of the water channels in the internodal cells ofNitellopsis . J. Membrain Biol. 116, 31–39 (1990). https://doi.org/10.1007/BF01871669
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DOI: https://doi.org/10.1007/BF01871669