Summary
The central problem for organisms which grow optimally, and in some cases obligately, at pH values of 10 to 11, is the maintenance of a relatively acidified cytoplasm. A key component of the pH homeostatic mechanism is an electrogenic Na+/H+ antiporter which—by virtue of kinetic properties and/or its concentration in the membrane—catalyzes net proton uptake while the organisms extrude protons during respiration. The antiporter is also capable of maintaining a constant pHin during profound elevations in pHout as long as Na+ entry is facilitated by the presence of solutes which are taken up with Na+. Secondary to the problem of acidifying the interior is the adverse effect of the large pH gradient, acid in, on the total pmf of alkalophile cells. For the purposes of solute uptake and motility, the organisms appear to largely bypass the problem of a low pmf by utilizing a sodium motive force for energization. However, ATP synthesis appears not to resolve the energetics problem by using Na+ or by incorporating the proton-translocating ATPase into intracellular organelles. The current data suggest that effective proton pumping carried out by the alkalophile respiratory chain at high pH may deliver at least some portion of the protons to the proton-utilizing catalysts, i. e., theF 1 F 0-ATPase and the Na+/H+ antiporter, by some localized pathway.
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Ando, A., Trie, S., Masuda, L.M., Matsushita, T., Fujji, T., Yabuki, M., Kusaka, T. 1983.Biochim. Biophys. Acta 734:290–294
Ando, A., Yabuki, M., Kusaka, J. 1981.Biochim. Biophys. Acta 640:179–184
Aronson, P.S., Nee, J., Suhm, M.A. 1982.Nature (London) 299:161–162
Bassilana, M., Damiano, E., Leblanc, G. 1984.Biochemistry 23:1015–1022
Bonner, S., Mann, M.J., Guffanti, A.A., Krulwich, T.A. 1982.Biochim. Biophys. Acta 679:315–322
Booth, J.R., Kroll, R.G. 1983.Biochem. Soc. Trans. 11:70–72
Chernyak, B.V., Dibrov, P.A., Glagolev, H.N., Sherman, M.Y., Skulachev, V.P. 1983.FEBS Lett. 164:38–42
Crane, R.K. 1977.Rev. Physiol. Biochem. Pharmacol. 78:99–159
DasSarma, S., Rajbhandary, V.L., Khorana, H.G. 1983.Proc. Natl. Acad. Sci. USA 80:2201–2205
Ferguson, S.J., 1985.Biochim. Biophys Acta 811:47–95
Garcia, M.L., Guffanti, A.A., Krulwich, T.A. 1983.J. Bacteriol. 156:1151–1157
Garland, P.B. 1977.Symp. Soc. Gen. Microbiol. 27:1–21
Gee, J.M., Lund, B.M., Metcalf, G., Peel, J.L. 1980.J. Gen. Microbiol. 117:9–17
Guffanti, A.A. 1983.FEMS Microbiol. Lett. 17:307–310
Guffanti, A.A. Blanco, R., Benenson, R.A., Krulwich, T.A. 1980.J. Gen. Microbiol. 119:79–86
Guffanti, A.A., Blanco, R., Krulwich, T.A. 1979.J. Biol. Chem. 254:1033–1037
Guffanti, A.A., Blumenfeld, H., Krulwich, T.A. 1981a J. Biol. Chem. 256:8416–8421
Guffanti, A.A., Bornstein, R.F., Krulwich, T.A. 1981b.Biochim. Biophys. Acta 635:619–630
Guffanti, A.A., Chiu, E., Krulwich, T.A. 1985.Arch. Biochem. Biophys. 237:327–333
Guffanti A.A., Cohn, D.E., Kaback, H.R., Krulwich, T.A. 1981a Proc. Natl. Acad. Sci. USA 78:1481–1484
Guffanti, A.A., Eisenstein, H.C. 1983.J. Gen. Microbiol. 129:3239–3242
Guffanti, A.A., Fuchs, R.T., Schneier, M., Chiu, E., Krulwich, T.A. 1984.J. Biol. Chem. 259:2971–2975
Guffanti, A.A., Susman, P., Blanco, R., Krulwich, T.A. 1978.J. Biol. Chem. 253:708–715
Hamaide, F. Kushner D.J., Sprott, G.D. 1983.J. Bacteriol. 156:537–544
Harold, F.M. 1977.Curr. Topics Bioenerg. 6:83–115
Hirata, H., Kambe, T., Kagawa, Y. 1984.J. Biol. Chem. 259:10653–10656
Hirota, N., Imae, Y. 1983.J. Biol. Chem. 258:10577–10581
Hirota, N., Kitada, M., Imae, Y. 1981.FEBS Lett. 132:278–280
Hoddinott, M.H., Reid, G.A., Ingledew, W.J. 1978.Biochem. Soc. Trans. 6:1295–1298
Honda, H., Kudo, T., Horikoshi, K. 1985.J. Bacteriol. 161:784–785
Horikoshi, K., Akiba, T. 1982. Alkalophilic Microorganisms. A New Microbial World. Springer-Verlag. New York
Kaczorowski, G.J., Kaback, H.R. 1979.Biochemistry 19:369–3697
Kallas, T., Castenholz, R.W. 1982.J. Bacteriol. 149:237–246
Kashket, E.R., Blanchard, A.G., Metzger, W.C. 1980.J. Bacteriol. 143:128–134
Kitada, M., Guffanti, A.A., Krulwich, T.A. 1982.J. Bacteriol. 152:1096–1104
Kitada, M., Horikoshi, K. 1977.J. Bacteriol. 131:784–788
Kitada, M., Horikoshi, K. 1979.Agric. Biol. Chem. 45:2273–2277
Kitada, M., Horikoshi, K. 1980a.J. Biochem. 87:1279–1284
Kitada, M., Horikoshi, K. 1980b.J. Biochem. 88:1757–1764
Kitada, M., Krulwich, T.A. 1984.J. Bacteriol. 158:965–966
Kitada, M., Lewis, R.J., Krulwich, T.A. 1983.J. Bacteriol. 154:330–335
Koga, Y., Nishihara, N., Morii, H. 1982.J. Univ. Occupat. Environ. Health 4:227–240
Koyama, N., Kiyamiya, A., Nosoh, Y. 1976.FEBS Lett. 72:77–78
Koyama, N., Koshiya, K., Nosoh, Y. 1980.Arch. Biochem. Biophys. 199:105–109
Koyama, N., Nosoh, Y. 1985.Biochim. Biophys. Acta 812:206–212
Koyama, N., Takinishi, H., Nosoh, Y. 1983.FEMS Microbiol. Lett. 16:213–216
Kroll, R.G., Booth, I.R. 1983.Biochem. J. 216:709–716
Krulwich, T.A. 1982.FEMS Microbiol. Lett. 15:299–301
Krulwich, T.A. 1983.Biochem. Biophys. Acta 726:245–264
Krulwich, T.A., Agus, R., Schneier, M., Guffanti, A.A. 1985a.J. Bacteriol. 162:768–772
Krulwich, T.A., Federbush, J.G., Guffanti, A.A. 1985b.J. Biol. Chem. 260:4055–4058
Krulwich, T.A., Guffanti, A.A. 1983.Adv. Microb. Physiol. 24:173–214
Krulwich, T.A., Guffanti A.A., Bornstein, R.F., Hoffstein, J. 1982.J. Biol. Chem. 257:1885–1889
Krulwich, T.A., Mandel, K.G., Bornstein, R.F., Guffanti, A.A. 1979.Biochem. Biophys. Res. Commun. 91:58–62
Kudo, T., Kato, C., Horikoshi, K. 1983.J. Bacteriol. 156:949–951
Kudo, T., Yoshitake, J., Kato, C., Usami, R., Horikoshi, K. 1985.J. Bacteriol. 161:158–163
Lanyi, J.K.. 1979.Biochim. Biophys. Acta 559:377–397
Lewis, R.J., Belkina, S., Krulwich, T.A. 1980.Biochem. Biophys. Res. Commun. 95:857–863
Lewis, R.J., Kaback, E., Krulwich, T.A. 1982.J. Gen. Microbiol. 128:427–930
Lewis, R.J., Krulwich, T.A., Reynafarje, B., Lehninger, A.L. 1983.J. Biol. Chem. 258:2109–2111
Lewis, R.J., Prince, R.C., Dutton, P.L., Knaff, D.B., Krulwich, T.A. 1981.J. Biol. Chem. 256:10543–10549
Mandel, K.G., Guffanti A.A. Krulwich, T.A. 1980.J. Biol. Chem. 255:7391–7396
McLaggan, D., Selwyn, M.J., Dawson, A.P. 1984.J. Bacteriol. 159:100–106
Mitchell, P. 1961.Nature (London) 191:144–148
Mitchell, P. 1963.Biochem. Soc. Symp. 22:142–168
Nakamura, T., Tokuda, H., Unemoto, T. 1984.Biochim. Biophys. Acta 776:330–336
Newman, M.J., Foster, D.L., Wilson, T.H., Kaback, H.R. 1981.J. Biol. Chem. 256:11804–11808
Niiya, S., Yamasaki, K., Wilson, T.H., Tsuchiya, T. 1982.J. Biol. Chem. 257:8902–8906
Nishihara, N., Morii, H., Koga, Y. 1982.J. Biochem. 92:1469–1479
Pfeifer, F., Betlach, M., Martienssen, R., Friedman, J., Boyer, H.W. 1983.Mol. Gen. Genet. 191:182–188
Ramos, S., Schuldiner, S., Kaback, H.R. 1976.Proc. Natl. Acad. Sci. USA 73:1892–1896
Rottenberg, H. 1984.J. Membrane Biol. 81:127–138
Rowland, G.C., Giffard, P.M., Booth, I.R. 1984.FEBS Lett.173:295–300
Schuldiner, S., Kaback, H.R. 1975.Biochemistry 14:5451–5461
Seto-Young, D., Garcia, M.L., Krulwich T.A. 1985.J. Biol. Chem. 260:11393–11395
Shioi, J.-I., matsuura, S., Imae, Y. 1980.J. Bacteriol. 144:891–897
Shiota, S., Yazyu, H., Tsuchiya, T., 1984.J. Bacteriol. 160:445–447
Skulachev, V.P. 1982.FEBS Lett. 146:1–4
Skulachev, V.P. 1984.Trends Biochem. Sci. 9:483–485
Strekas, J.C., 1984.Biochim. Biophys. Acta 765:133–137
Sugiyama, S., Matsukura, H., Imae, Y. 1985.FEBS Lett. 182:265–268
Takinishi, H., Sekiguchi, T., Koyama, M., Shishido, K., Nosohi, Y. 1983.FEBS Lett. 154:201–204
Tindall, B.J., Mills, A.A., Grant, W.D. 1980.J. Gen Microbiol. 116:257–260
Tokuda, H., Unemoto, T. 1981.Biochem. Biophys. Res. Commun. 102:265–271
Tokuda, H., Unemoto, T. 1982.J. Biol. Chem. 257:10007–10014
Westerhoff, H.V., Melandri, B.A., Venturoli, G., Azzone, G.T., Kell, D.B. 1984.Biochem. Biophys. Acta 768:257–292
Zilberstein, D., Agmon, V., Schuldiner, S., Padan, E. 1982a.J. Biol. Chem. 257:3687–3691
Zilberstein, D., Agmon, V., Schuldiner, S., Padan, E., 1984.J. Bacteriol. 158:246–252
Zilberstein, D., Ophir, T.Y., Padan, E., Schuldiner, S. 1982b.J. Biol. Chem. 257:3692–3696
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Krulwich, T.A. Bioenergetics of alkalophilic bacteria. J. Membrain Biol. 89, 113–125 (1986). https://doi.org/10.1007/BF01869707
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DOI: https://doi.org/10.1007/BF01869707