Summary
Oncogenic human papillomavirus (HPV) types 16 and 18 commonly associated with cervical cancer are found in many epithelial malignancies at extra-genital sites including breast. The transforming gene products of HPV have also been shown to immortalize breast epithelial cellsin vitro. But the findings of HPV DNA in breast carcinoma are found to be contradictory. In the present study fine needle aspirate cell (FNAC) samples from 26 breast cancer patients and four breast tumour biopsies were analysed for the presence of HPV 16 and 18 DNA sequences by both polymerase chain reaction (PCR) and Southern blot hybridization. Of 26 fine needle aspi rate cell samples and four breast cancer biopsies, not a single sample was found to be positive by either PCR or Southern blot hybridization. The observation of complete absence of HPV DNA sequences in breast cancer refute the possibility of any role for oncogenic genital HPV types 16 and 18 in the pathogenesis of breast cancer.
Article PDF
Avoid common mistakes on your manuscript.
References
De Villiers EM: Heterogeneity of the human papillomavirus group. J Virol 63: 4898–4903, 1989
Beckmann AM, Daling JR, Sherman KJ, Maden C, Miller BA, Coates RJ, Kiviat NB, Myerson D, Weiss NS, Hislop TG, Beagrie M, Mc Dougall JK: Human papillomavirus infection and anal cancer. Int J Cancer 43: 1042–1049, 1989
Scheurlen W, Stremlan A, Gissmann L, Hohn D, Zenner HP, zur Hausen H: Rearranged HPV 16 molecules in an anal and in a laryngeal carcinoma. Int J Cancer 38: 671–676, 1986
Perez-Ayala M, Ruiz-Cabello F, Esteban F, Concha A, Redondo M, Oliva MR, Cabrera T, Garrido F: Presence of HPV 16 sequences in laryngeal carcinoma. Int J Cancer 46: 8–11, 1990
Guitart J, Bergfeld WF, Tuthill RJ, Tubbs RR, Zienowicz R, Fleegler EJ: Squamous cell carcinoma of the nail bed: a clinicopathological study of 12 cases. Brit J Dermatol 123: 215–222, 1990
Pfister H: Human papillomavirus and genital cancer (review). Adv Cancer Res 48: 1–26, 1987
Vousden KH: Human papillomaviruses and cervical carcinoma. Cancer Cells 1: 43–50, 1989
zur Hausen H: Papillomaviruses as carcinoma viruses. Adv Viral Oncol 8: 1–26, 1989
Das BC, Sehgal A, Murthy NS, Gopalkrishna V, Sharma JK, Das DKet al.: Human papillomavirus and cervical cancer in Indian women. Lancet 2: 1271, 1989
Hawley-Nelson P, Vousden KH, Hubbert NI, Lowy DR, Schiller JT: HPV 16 E6 and E7 proteins cooperate to immortalise human foreskin keratinocytes. EMBO J 8: 3905–3910, 1989
Munger RK, Phelps WC, Bubb V, Howley PM, Schlegel R: The E6 and E7 genes of the human papillomavirus type 16 together are necessary for transformation of primary human keratinocytes. J Virol 63: 4417–4421, 1989
Hudson JB, Bedell MA, Mc Cance DJ, Laminis LA: Immortalisation and altered differentiation of human keratinocytesin vitro by the E6 and E7 open reading frames of human papillomavirus type 18. J Virol 64: 519–526, 1990
Band V, Zaichowski D, Kulesa V, Sager R: Human papillomavirus DNA's immortalise normal epithelial cells and reduce their growth factor requirements. Proc Natl Acad Sci USA 87: 463–467, 1990
Band V, De Caprio JA, Delmolino L, Kulesa V, Sager R: Loss of p53 protein in human papillomavirus type 16 E6-immortalised human mammary epithelial cells. J Virol 65: 6671–6676, 1991
Chang F, Syrjanen S, Shen Q, Ji HX, Syrjanen K: Human papillomavirus (HPV) DNA in esophageal precancer lesions and squamous cell carcinomas from China. Int J Cancer 45: 21–25, 1990
Syrjanen KJ: Human papillomavirus infections in the oral cavity. In: Syrjanen K, Gissmann L, Koss LG (eds) Papillomaviruses and Human Disease. Heidelberg, Springer-Verlag: 104–137, 1987
Di Lonardo A, Venuti A, Marcante ML: Human papillomavirus in breast cancer. Breast Cancer Res Treat 21: 95–100, 1992
Wrede D, Luqmani YA, Coombes RC, Vousden KH: Absence of HPV 16 and 18 DNA in breast cancer. Br J Cancer 65: 891–894, 1992
Das BC, Sharma JK, Gopalkrishna V, Das DK, Singh V, Gissmann Let al.: A high frequency of human papillomavirus DNA sequences in cervical carcinomas of Indian women as revealed by Southern blot hybridization and polymerase chain reaction. J Med Virol 36: 239–245, 1992
Gopalkrishna V, Francis A, Sharma JK, Das BC: A simple and rapid method of high quantity of DNA isolation from cervical scrapes for detection of human papillomavirus infection. J Virol Meth 36: 63–72, 1992
Saiki RK, Scharf S, Faloona F, Mullis KB, Horn GT, Erlich HA, Arnheim N: Enzymatic amplification of β-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 230: 1350–1354, 1985
Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA: Primer directed enzymatic amplification of DNA with thermostable DNA polymerase. Science 239: 487–491, 1988
Sharma JK, Gopalkrishna V, Das BC: A simple method of elimination of unspecific amplifications in polymerase chain reaction. Nucleic Acids Res 20: 6117–6118, 1992
Das BC, Sharma JK, Gopalkrishna V, Luthra UK: Analysis by polymerase chain reaction of the physical state of human papillomavirus type 16 DNA in cervical preneoplastic and neoplastic lesions. J Gen Virol 73: 2327–2336, 1992
Southern EM: Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98: 503–517, 1975
Ostrow RS, Manias DA, Fong WJ, Zachow KR, Faras AJ: A survey of human cancers for human papillomavirus DNA by filter hybridization. Cancer 59: 429–434, 1987
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gopalkrishna, V., Singh, U.R., Sodhani, P. et al. Absence of human papillomavirus DNA in breast cancer as revealed by polymerase chain reaction. Breast Cancer Res Tr 39, 197–202 (1996). https://doi.org/10.1007/BF01806186
Issue Date:
DOI: https://doi.org/10.1007/BF01806186