Summary
Techniques for the isolation and culture of human mammary epithelial cells are described. The isolation procedure consists of dissection followed by partial enzymatic digestion with collagenase and hyal-uronidase and subsequent filtration to separate the epithelial clusters from the digested stromal elements. Culture procedures utilizing two different growth media are presented. A serum-free medium, MCDB170, permits long-term growth (45 to 60 population doublings) of a pure epithelial population; a less defined medium, MM, yields fewer population doublings but increased expression of some mammary-specific properties.
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Stampfer, M.R. Isolation and growth of human mammary epithelial cells. Journal of Tissue Culture Methods 9, 107–115 (1985). https://doi.org/10.1007/BF01797781
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DOI: https://doi.org/10.1007/BF01797781