Summary
The sarcoplasmic concentrations of phosphorus metabolites and pH (pHin) were measured in the anterior byssus retractor muscle (ABRM) ofMytilus edulis by31P nuclear magnetic resonance spectroscopy. During an active contraction induced by 10−3 m acetylcholine, the concentration of arginine phosphate ([Arg-P]in) decreased from the resting value of 7.47±0.26 (mean±se,n=8) to 6.67±0.29 (n=6) μmol g−1, and that of inorganic phosphate (Pi) consistently increased from 0.84±0.06 (n=7) to 1.61±0.12 (n=5) μmol g−1. In the ‘catch’ state following the active contraction, these concentrations were close to their resting levels, indicating that the catch is an inactive state. 5-hydroxytryptamine caused a rapid relaxation of the catch, which was associated with a slight decrease in [Arg-P]in and an increase in pHin by ca 0.2 units. The sarcoplasmic concentration of ATP (mean, 1.6μmol g−1) did not change throughout the contraction-relaxation cycle.
Article PDF
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
References
Achazi, R. K. (1979) Phosphorylation of molluscan paramyosin.Pflügers Arch. 379, 197–201.
Ashley, C. C., Ishii, N. &Simpson, A. W. M. (1989) Intracellular pH (pHi) is not altered by agents which promote contraction and relaxation in smooth muscle cells isolated from the ABRM ofMytilus edulis.J. Physiol. (Lond.) 415, 102P.
Baguet, F. &Gillis, J. M. (1968) Energy cost of tonic contraction in a lamellibranch catch muscle.J. Physiol. (Lond.) 198, 127–43.
Castellani, L. &Cohen, C. (1987) Myosin rod phosphorylation and the catch state of molluscan muscles.Science 235, 335–7.
Cooley, L. B., Johnson, W. H. &Krause, S. (1979) Phosphorylation of paramyosin and its possible role in the catch mechanism.J. biol. Chem. 256, 3178–81.
Cornelius, F. (1982) Tonic contraction and the control of relaxation in a chemically skinned molluscan smooth muscle.J. Gen. Physiol. 79, 821–34.
Ellington, W. R. (1983) The extent of intracellular acidification during anoxia in the catch muscle of two bivalve molluscs.J. Exp. Zool. 227, 313–7.
Gies, A. (1988) Changes of metabolite contents and of energy charge induced by contraction, catch and relaxation in smooth molluscan muscle fibres. An analysis using reversed-phase ion-pair high-performance liquid chromatography.Comp. Biochem. Physiol. 91B, 483–7.
Goldman, Y. E. (1987) Kinetics of the actomyosin ATPase in muscle fibers.Ann. Rev. Physiol. 49, 637–54.
Ishii, N. (1987) Mechanical properties of saponin-treated smooth muscle cells isolated from a molluscan smooth muscle.J. Musc. Res. Cell Motil. 8, 281.
Ishii, N., Mitsumori, F., Takahashi, K., Simpson, A. W. M. &Ashley, C. C. (1989a) Intracellular metabolite and free calcium concentrations during the ‘catch’ contraction and relaxation in a molluscan smooth muscle. InMuscle Energetics (edited byPaul, R. J., Elzinga, G. &Yamada, K.) pp. 463–4. New York: Alan R. Liss.
Ishii, N., Simpson, A. W. M. &Ashley, C. C. (1989b) Free calcium at rest during “catch” in single smooth muscle cells.Science 243, 1367–8.
Ishii, N., Simpson, A. W. M. &Ashley, C. C. (1989c) Effects of 5-hydroxytryptamine (serotonin) and forskolin on intracellular free calcium in isolated and fura-2 loaded smooth-muscle cells from the anterior byssus retractor (catch) muscle ofMytilus edulis.Pflügers Arch. 414, 162–70.
Jewell, B. R. (1959) The nature of the phasic and tonic responses of the anterior byssal retractor muscle ofMytilus.J. Physiol. (Lond.) 149, 154–77.
Lowy, J. B. M. &Millman, B. M. (1963) The contractile mechanism of the anterior byssus retractor muscle (ABRM) ofMytilus edulis.Phil. Trans. R. Soc. (Lond.) B246, 105–48.
Marchand-Dumont, G. &Baguet, F. (1975). The control mechanism of relaxation in molluscan catch muscle (ABRM).Pflügers Arch. 354, 87–100.
Nauss, K. &Davies, R. E. (1966) Changes in inorganic phosphate and arginine during the development, maintenance and loss of tension in the anterior byssus retractor muscle ofMytilus edulis.Biochem. Z. 345, 173–87.
Pfitzer, G. &Rüegg, J. C. (1982) Molluscan catch muscle: Regulation and mechanics in living and skinned anterior byssus retractor muscle ofMytilus edulis.J. Comp. Physiol. 147, 137–42.
Rüegg, J. C. (1971) Smooth muscle tone.Physiol. Rev. 51, 201–48.
Rüegg, J. C. (1986)Calcium in Muscle Activation. Berlin: Springer.
Schanck, A., Verbaert, B., Van Meersch, M., Baguet, F. &Devroede J. (1986)31P-nuclear-magnetic resonance study of muscles fromMytilus edulis.Eur. J. Biochem. 156, 625–9.
Sohma, H., Yazawa, M. &Morita, F. (1985) Phosphorylation of regulatory light chain a (RLC-a) in smooth muscle myosin of scallop,Papinopecten yessoensis.J. Biochem. (Tokyo) 98, 569–72.
Sohma, H., Inoue, K. &Morita, F. (1988) A cAMP-dependent regulatory protein for RLC-a myosin kinase catalyzing the phosphorylation of scallop smooth muscle myosin light chain.J. Biochem. (Tokyo) 103, 431–5.
Trentham, D. R., Eccleston, J. F. &Bagshaw, C. R. (1976) Kinetic analysis of ATPase mechanisms.Quart. Rev. Biophys. 9, 217–81.
Twarog, B. M. (1976) Aspects of smooth muscle function in molluscan catch muscle.Physiol. Rev. 56, 829–38.
Winton, F. R. (1937) The changes in viscosity of an unstriated muscle (Mytilus edulis) during and after stimulation with alternating, interrupted and uninterrupted direct currents.J. Physiol. (Lond.) 88, 492–511.
Zange, J., Grieshaber, M. K. &Jans, A. W. (1990a) The regulation of intracellular pH estimated by31P-NMR spectroscopy in the anterior byssus retractor muscle ofMytilus edulis L.J. Exp. Biol. 150, 95–109.
Zange, J., Pörtner, H. O., Jans, A. W. H. &Grieshaber, M. K. (1990b) The intracellular pH of a molluscan smooth muscle during a contraction-catch-relaxation cycle estimated by the distribution of [14C]DMO and by31P-NMR spectroscopy.J. Exp. Biol. 150, 81–93.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ishii, N., Mitsumori, F. & Takahashi, K. Changes in sarcoplasmic metabolite concentrations and pH associated with the catch contraction and relaxation of the anterior byssus retractor muscle ofMytilus edulis measured by phosphorus-31 nuclear magnetic resonance. J Muscle Res Cell Motil 12, 242–246 (1991). https://doi.org/10.1007/BF01745113
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01745113