Summary
Using histochemical, immunohistochemical and biochemical techniques, noradrenaline-, neuropeptide Y-, vasoactive intestinal polypeptide-, substance P- and calcitonin gene-related peptide-containing nerve fibres were studied in the uterine artery of virgin, progesterone-treated and pregnant guinea-pigs. Morphological changes following hormone treatment or in pregnancy were also evaluated in a quantitative study on semithin sections of the uterine artery. In late pregnancy, the number of noradrenalinecontaining nerve fibres, which formed the densest plexus in virgin animals, was significantly decreased, a finding supported by a significant reduction in noradrenaline levels. This reduction was not mimicked by systemic progesterone treatment. In contrast, the innervation of the uterine artery by neuropeptide Y-containing nerve fibres was increased in pregnancy, while the other peptidergic nerves and peptide levels were unchanged after progesterone treatment and in pregnancy. These changes led to a predominance of innervation by neuropeptide Y- rather than noradrenaline-containing nerve fibres in late pregnancy. No morphological changes were detected following progesterone treament, but pregnancy led to a marked increase in the cross-sectional area of the vessel accompanied by an increase in the thickness of the media.
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Alm P, Björklund A, Owman C, Thorbert G (1979) Tyrosine hydroxylase and DOPA decarboxylase activities in the guinea pig uterus. Further evidence for functional adrenergic denervation in association with pregnancy. Neuroscience 4:145–154
Belai A, Lincoln J, Milner P, Crowe R, Loesch A, Burnstock G (1985) Enteric nerves in diabetic rats: increase in vasoactive intestinal polypeptide but not substance P. Gastroenterology 89:967–976
Bell C (1968) Dual vasoconstrictor and vasodilator innervation of the uterine arterial supply in the guinea pig. Circ Res 23:269–279
Bell C (1969) Fine structural localization of acetylcholinesterase at a cholinergic vasodilator nerve-arterial smooth muscle synapse. Circ Res 24:61–70
Bell C, Malcolm SJ (1978) Observation on the loss of catecholamine fluorescence from intrauterine adrenergic nerves during pregnancy in the guinea-pig. J Reprod Fertil 53:51–58
Cavanagh JFR, Mione MC, Burnstock G (1988) The peptidergic innervation of the uterine artery during pregnancy. Regul Pept 22:395
Clark KE, Mills EG, Stys SJ, Seeds AE (1981) Effects of vasoactive polypeptides on uterine vasculature. Am J Obstet Gynecol 139:182–188
Cowen T, Haven AJ, Burnstock G (1985) Pontamine Sky Blue: a counterstain for background autofluorescence and immunofluorescence histochemistry. Histochemistry 82:205–208
Draper RL (1920) The prenatal growth of the guinea pig. Anat Rec 18:369–392
Dunnet CW (1964) New table for multiple comparison with a control. Biometrics 20:482–491
Fried G, Hökfelt T, Terenius L, Goldstein M (1985) Neuropeptide Y (NPY)-like immunoreactivity in guinea pig uterus is reduced during pregnancy in parallel with noradrenergic nerves. Histochemistry 83:437–442
Gibbins IL, Morris JL (1988) Co-existence of immunoreactivity to neuropeptide Y and vasoactive intestinal polypeptide in nonnoradrenergic axons innervating guinea pig cerebral arteries after sympathectomy. Brain Res 444:402–406
Gibbins IL, Furness JB, Costa M, MacIntyre I, Hillyard CJ, Girgis S (1985) Colocalization of calcitonin gene-related peptide-like immunoreactivity with substance P in cutaneous, vascular and visceral sensory neurons of guinea pigs. Neurosci Lett 57:125–130
Girgis SI, MacDonald DW, Stevenson JC, Bevis PJR, Lynch C, Wimalawansa SJ, Self CH, Morris HR, MacIntyre I (1985) Calcitonin gene-related peptide: potent vasodilator and major product of calcitonin gene. Lancet 2:14–16
Gram BR, Ottesen B (1982) Increased myometrial blood flow evoked by substance P. Pflügers Arch 395:347–350
Humphrey CD, Pittman FE (1974) A simple methylene blue-Azur II-basic fuchsin stain for epoxy-embedded tissue sections. Stain Technol 49:9–14
Keller R, Oke A, Mefford I, Adams RN (1976) Liquid chromatographic analysis of catecholamines: routine assay for regional brain mapping. Life Sci 19:995–1004
Lee RMKW, Forrest JB, Garfield RE, Daniel EE (1983) Comparison of blood vessel wall dimensions in normotensive and hypertensive rats by histometric and morphometric methods. Blood Vessels 20:245–254
Lincoln J, Bokor JT, Crowe R, Griffith SG, Haven AJ, Burnstock G (1984) Myenteric plexus in streptozotocin-treated rats. Neurochemical and histochemical evidence for diabetic neuropathy in the gut. Gastroenterology 86:654–661
Lindvall O, Björklund A (1974) The glyoxylic acid fluorescence histochemical method: a detailed account of the methodology for the visualization of central catecholamine neurons. Histochemistry 39:97–127
Morris JL, Murphy R (1988) Evidence that neuropeptide Y released from noradrenergic axons causes prolonged contractions of the guinea-pig uterine artery. J Auton Nerv Syst 24:241–250
Morris JL, Gibbins IL, Furness JB, Costa M, Murphy R (1985) Colocalization of neuropeptide Y, vasoactive intestinal polypeptide and dynorphin in non-adrenergic axons of the guinea pig uterine artery. Neurosci Lett 62:31–37
Morris JL, Gibbins IL, Furness JB (1987) Increased dopamine-β-hydroxylase-like immunoreactivity in non-adrenergic axons supplying the guinea pig uterine artery after 6-hydroxydopamine treatment. J Auton Nerv Syst 21:15–27
Moyer TP, Jiang N-S (1978) Optimized isocratic conditions for analysis of catecholamines by high performance reversed-phase paired-ion chromatography with amperometric detection. J Chromatogr 153:365–372
Ottesen B, Fahrenkrug J (1981) Effect of vasoactive intestinal polypeptide (VIP) upon myometrial blood flow in the non-pregnant rabbit. Acta Physiol Scand 112:195–201
Owman C, Alm P, Rosengren E, Sjöberg N-O (1975) Variations in the level of uterine norepinephrine during pregnancy in guinea pig. Am J Obstet Gynecol 122:961–969
Sjöberg N-O (1968) Consideration on the cause of disappearance of the adrenergic neurotransmitter in the uterine nerves during pregnancy. Acta Physiol Scand 72:510–517
Sporrong B, Alm P, Owman C, Sjöberg N-O, Thorbert G (1981) Pregnancy is associated with extensive adrenergic nerve degeneration in the uterus. An electronmicroscopic study in the guinea pig. Neuroscience 6:1119–1126
Stjernquist M, Alm P, Ekman R, Owman C, Sjöberg N-O, Sundler F (1985) Levels of neural vasoactive polypeptide in rat uterus are markedly changed in association with pregnancy as shown by immunocytochemistry and radioimmunoassay. Biol Reprod 33:157–163
Stjernschantz J, Gregerson D, Bauscher L, Sears M (1982) Enzymelinked immuno-sorbent assay of substance P: a study of the eye. J Neurochem 38:1323–1328
Tare M, Parkington HC, Coleman HA (1988) Changes in the adrenergic innervation of the main uterine artery during pregnancy in guinea-pigs. Proc Aust Physiol Pharmacol Soc, Feb 1988:33P
Thorbert G, Alm P, Björklund A, Owman C, Sjöberg N-O (1979) Adrenergic innervation of the human uterus. Disappearance of the transmitter-forming enzymes during pregnancy. Am J Obstet Gynecol 135:223–226
Todd ME, Laye CG, Osborne DN (1983) The dimensional characteristics of smooth muscle in rat blood vessels. A computer assisted analysis. Circ Res 53:319–331
Uddman R, Edvinsson L, Ekblad E, Håkanson R, Sundler F (1986) Calcitonin gene-related peptide (CGRP): perivascular distribution and vasomotor effects. Regul Pept 15:1–23
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Mione, M.C., Cavanagh, J.F.R., Lincoln, J. et al. Pregnancy reduces noradrenaline but not neuropeptide levels in the uterine artery of the guinea-pig. Cell Tissue Res. 259, 503–509 (1990). https://doi.org/10.1007/BF01740777
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DOI: https://doi.org/10.1007/BF01740777