Summary
A series of five alternating poly(leucyl-lysyl) samples with varying amounts of L- and D-residues randomly distributed along the chain, but evenly shared out amongst leucyl and lysyl residues were synthesized by condensation of a mixture of the four diastereoisomeric dipeptidep-nitro-phenylesters. Their behavior in aqueous solution at various ionic strengths was studied by infrared spectroscopy which allowed measurement of the total amount ofβ-structures, and by circular dichroism which gives the excess of L-residues over D-residues in the same structures. Comparison with the properties of the all L-poly(Lys-Leu-Lys-Leu) shows that incorporation of a few D-residues in a L-chain seems to reduce the width of theβ-sheets obtained in presence of salt. Higher proportions of D-isomers prevent the coil →β transition from occurring when the ionic strength is increased except for segments containing at least 6 to 7 adjacent residues of the same configuration.
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Brack, A., Spach, G. β-structures of polypeptides with L- and D-residues. J Mol Evol 13, 35–46 (1979). https://doi.org/10.1007/BF01732752
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DOI: https://doi.org/10.1007/BF01732752