Summary
A viroid was isolated from symptomlessColeus blumei cultivar (cv.) ‘Rainbow Gold’ plants using the bidirectional PAGE method for analysis of small circular RNA molecules. The viriod was transmitted to viroid-free plants ofColeus blumei cv. ‘Scarlet Dragonfly’ by mechanical inoculation. Cloning and sequencing revealed that the viriod from theColeus cv. ‘Rainbow Gold’ is closely related to theColeus blumei viriod 1-BvA (CbVd 1-BvA) isolated from theColeus cv. ‘Bienvenue’. Therefore, new viroid sequence variant has been namedColeus blumei viriod 1-RG (CbVd 1-RG).Coleus blumei viriod 1-RG consists of 251 nucleotides, 140 G + C, 111 A + U with a GC content of 55.4%. The most stable rod-like secondary structure of this viroid has 53 G:C, 29 A:U and 5 G:U base pairs with a minimum free energy (at 25°C) of − 81.2 kcal/mol (− 339.4 kJ/mol). The right terminal domain shows a high sequence similarity to the corresponding domain of hop latent viroid (HLVd).
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References
Diener TO (ed) (1987) The viroids. Plenum Press, New York
Diener TO (1991) The frontier of life: the viroid and viroid-like satellites RNAs. In: Maramorosch K (ed) Viroids and satellites: molecular parasites at the frontier of life. CRC Press, Boca Raton, pp 1–20
Fonseca MEN, Boiteux LS, Singh RP, Kitajima EW (1989) A small viroid inColeus species from Brazil. Fitopatol Brasil 14: 94–96
Fonseca MEN, Boiteux LS, Singh RP, Kitajima EW (1990) A viroid fromColeus species in Brazil. Plant Dis 74: 80
Fonseca MEN, Marcellino LH, Kitajima EW, Boteux LS (1994) Nucleotide sequence of the original Brazilian isolate of coleus yellow viroid fromSolenostemon scutellaroides and infectivity of its complementary DNA. J Gen Virol 75: 1447–1449
Gross HJ, Krupp G, Domedy H, Raba M, Alberty H, Lossow CH, Ramm K, Sänger HL (1982) Nucleotide sequence and secondary structure of citrus exocortis and chrysanthemum stunt viroid. Eur J Biochem 121: 249–257
Hammond R, Smith DR, Diener T (1989) Nucleotide sequence and proposed secondary structure ofColumnea latent viroid: a natural mosaic of viroid sequences. Nucleic Acids Res 23: 10083–10094
Herold T, Haas B, Singh RP, Boucher A, Sänger HL (1992) Sequence analysis of five new field isolates demonstrates that the chain length of potato spindle tuber viroid (PSTVd) is not strictly conserved but as variable as in other viroids. Plant Mol Biol 19: 329–333
Keese P, Symons RH (1985) Domains in viroids: evidence of intermolecular rearrangements and their contribution to viroid evolution. Proc Natl Acad Sci USA 82: 4582–4586
Koltunow AM, Rezaian MA (1989) A scheme of viroid classification. Intervirology 30: 194–201
Maxam AM, Gilbert W (1989) Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol 65: 499–560
Ohno T, Takamatsu N, Meshi T, Okada Y (1983) Hop stunt viroid: molecular cloning and nucleotide sequence of the complete cDNA copy. Nucleic Acids Res 11: 6185–6197
Puchta H, Ramm K, Sänger HL (1988) The molecular structure of the hop latent viroid (HLV), a new viroid occuring worldwide in hops. Nucleic Acids Res 16: 4197–4216
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, New York
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467
Sänger HL, Klotz G, Riesner D, Gross HJ, Kleinschmidt AK (1976) Viroids are single-stranded covalently closed circular RNA molecules existing as highly base-paired rod-like structures. Proc Natl Acad Sci USA 73: 3852–3856
Sänger HL (1987) Viroid replication. In: Diener TO (ed) The viroids. Plenum Press, New York, pp 117–166
Schnölzer M, Haas B, Ramm K, Hofmann H, Sänger HL (1985) Correlation between structure and pathogenicity of potato spindle tuber viroid (PSTV). EMBO J 4: 2181–2190
Schumacher J, Randles JW, Riesner D (1983) A two-dimensional electrophoresis technique for the detection of circular viroids and virusoids. Anal Biochem 135: 288–295
Semancik JS (ed) (1987) Viroids and viroid-like pathogens. CRC Press, Boca Raton
Singh RP, Boucher A, Singh A (1991) High incidence of transmission and occurrence of a viroid in commercial seeds ofColeus in Canada. Plant Dis 75: 184–187
Singh RP, Lakshman DK, Boucher A, Tavantizis SM (1992) A viroid fromNematanthus wettsteinii plants closely related to theColummnea latent viroid. J Gen Virol 73: 2769–2774
Spieker RL, Haas B, Charng YC, Freimüller K, Sänger HL (1990) Primary and secondary structure of a new viroid ‘species’ (CbVd 1) present in theColeus blumei cultivar ‘Bienevenue’. Nucleic Acids Res 18: 3998
Spieker RL (1991) A new class of viroids inColeus blumei: sequencing, cloning, structure/function-analusis by site-directed mutagenesis and expression inNicotiana tabacum. Karlsruhe Contr Plant Phys 22: 1–226
Spiesmacher E, Mühlbach H-P, Table M, Sänger HL (1985) Oligomeric forms of potato spindle tuber viroid (PSTV) and its complementary RNA are present in nuclei isolated from viroid-infected potato cells. Biosci Rep 5: 251–265
Symons RH (1990) The fascination of low molecular weight pathogenic RNAs. Semin Virol 1: 75–81
Tabler M, Sänger HL (1984) Cloned single and double-stranded DNA copies of potato spindle tuber viroid (PSTV) and coinoculated subgenomic DNA fragments are infectious. EMBO J 3: 3055–3062
Zuker M (1989) On finding all suboptimal foldings of an RNA molecule. Science 244: 48–52
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The sequence data reported here have been submitted to the EMBL database and have been assigned the accession numbers X95291 (for CbVd 1-RG) and X57284 (for CbVd 1-BvA).
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Spieker, R.L. A new sequence variant ofColeus blumei viroid 1 from theColeus blumei cultivar ‘Rainbow Gold’. Archives of Virology 141, 2153–2161 (1996). https://doi.org/10.1007/BF01718222
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DOI: https://doi.org/10.1007/BF01718222