Summary
The influence of serum from patients with essential hypertension on the sodium efflux rate constants of human lymphocytes and on the activity of isolated (Na++K+)-ATPase was investigated. The ouabain-sensitive sodium efflux rate constant was significantly decreased (p<0.001) in the sera of 19 hypertensives (1.92±0.11 h−1) compared with the sera of 30 normotensives (2.44±0.07 h−1). The ouabain-insensitive sodium efflux was unaffected. These results corresponded with a significant difference (p<0.005) of (Na++K+)-ATPase activity (1.03±0.04 mU/ml and 0.079±0.06 mU/ml), when an isolated (Na++K+)-ATPase was incubated with the sera of 22 normotensives or 18 hypertensives. Both the rate constant of ouabain-sensitive sodium efflux and the (Na++K+)-ATPase activity correlated significantly with the diastolic and systolic blood pressure (p<0.001). These data, therefore, demonstrated the close relationship between essential hypertension and the concentration of a circulating inhibitor of the sodium pump.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- ATP:
-
Adenosine triphosphate
- EGTA:
-
Ethyleneglycol bis(2-aminoethyl)-N,N,N′,N′-tetraacetic acid
References
Akagawa K, Hara N, Tsukada Y (1984) Partial purification and the characters of the inhibitors to NaK-ATPase and ouabain binding in the bovine central nervous system. J Neurochem 42:775–780
Ambrosioni E, Costa FV, Montebugnoli L, Tartagni F, Magnani B (1982) Increased intralymphocytic sodium content in essential hypertension: An index of impaired Na+ cellular metabolism. Clin Sci 61:181–186
Beretta-Piccoli C, Davies DL, Boddy K, Brown JJ, Cumming AMM, East BW, Fraser R, Lever AF, Padfield PL, Semple PF, Robertson JIS, Weidmann P, Williams ED (1982) Relation of arterial pressure with body sodium, body potassium and plasma potassium in essential hypertension. Clin Sci 63:257–270
Canessa M, Adragna N, Solomon HS, Conolly TM, Tosteson DC (1980) Increased sodium-lithium countertransport in red cells of patients with essential hypertension. New Engl J Med 302:772–776
Canessa M, Bize I, Solomon H, Adragna N, Tosteson DC, Dagher G, Garay R, Meyer P (1981) Na countertransport and cotransport in human red cells: function, dysfunction and genes in essential hypertension. Clin Exp Hypertens 3:783–795
Crabos M, Wainer IM, Cloix JF (1984) Measurement of endogenous Na+, K+-ATPase inhibitors in human plasma and urine using high performance liquid chromatography. FEBS Lett 176:223–228
Duhm J, Göbel BO, Lorenz R, Weber PC (1982) Sodium-lithium exchange and sodium-potassium cotransport in human erythrocytes. Part II: A simple uptake test applied to normotensive and essential hypertensive individuals. Hypertension 4:477–482
Ficoll-Paque™. For in vitro isolation of lymphocytes. Pharmacia Fine Chemicals, Uppsala, Sweden
Fishman MC (1979) Endogenous digitalis-like activity in mammalian brain. Proc Natl Acad Sci USA 76:4661–4663
Forrester T, Alleyne GAO (1981) Sodium, potassium and rate constants for sodium efflux in leukocytes from hypertensive Jamaicans. Br Med J 283:5–7
Garay RP, Dagher G, Pernollet MG, Devynck MA, Meyer P (1980) Inherited defect in a (Na-K) cotransport system in erythrocytes from essential hypertensive patients. Nature 284:281–282
Glynn IM, Karlish SJD (1975) The sodium pump. Annu Rev Physiol 37:13–55
Gruber KA, Rudel LL, Bullock BC (1982) Increased circulating levels of an endogenous digoxin-like factor in hypertensive monkeys. Hypertension 4:348–354
Hamlyn JM, Ringel R, Schaeffer J, Levinson PD, Hamilton BP, Kowarski AA, Blaustein MP (1982) A circulating inhibitor of (Na++K+)-ATPase associated with essential hypertension. Nature 300:650–652
Haddy FJ, Pamnani MB (1984) The vascular Na+−K+ pump in low renin hypertension. J Cardiovasc Pharmacol 6:S61-S74
Harris R, Ukaejiofo EO (1970) Tissue typing using a routine one-step lymphocytes separation technique. Br J Haematol 18:229–235
Haupert GT, Sancho JM (1979) Sodium transport inhibitor from bovine hypothalamus. Proc Natl Acad Sci USA 76:4658–4660
Henning G, Cloix JF (1983) Chromatographie d'affinité pour la détection d'un inhibiteur endogène humain de la Na+, K+-ATPase. CR Acad Sc Paris 297: Série III, 295–298
Hilton PJ, Patrick J (1973) Sodium and potassium flux rates in normal human lymphocytes in an artificial extracellular fluid. Clin Sci 44:439–445
Jørgensen PL (1974) Purification and characterization of (Na++K+)-ATPase. III. Purification from the outer medulla of mammalian kidney after selective removal of membrane components by sodium dodecylsulphate. Biochim Biophys Acta 356:36–52
Kramer HJ, Krück F (1982) Störung des Membran-Transportes bei der Hypertonie. Münch Med Wochenschr 124:1055–1060
Kuske R, Renner D, Schoner W (1984) Demonstration of an inhibitor of the sodium pump of human lymphocytes in the serum of patients with essential hypertension. Biomed Biochim Acta 43:S21-S22
Lichstein D, Samuelov S (1980) Endogenous “ouabainlike” activity in rat brain. Biochem Biophys Res Commun 96:1518–1523
MacGregor GA, Fenton S, Alaghband-Zadeh J, Markandu N, Roulston JE, De Wardener HE (1981) Evidence for a raised concentration of a circulating sodium transport inhibitor in essential hypertension. Br Med J 283:1355–1360
Martin J, Doty DM (1949) Determination of inorganic phosphate: Modification of isobutyl alcohol procedure. Anal Chem 21:965–967
Morgan T, Meyers J, Fitzgibbon W (1981) Sodium intake, blood pressure and red cell sodium efflux. Clin Exp Hypertens 3:641–653
Postnov YV, Orlov SN, Shevchenko A, Adler AM (1977) Altered sodium permeability, calcium binding and Na-K-ATPase activity in the red cell membrane in essential hypertension. Pflüger's Arch 371:263–269
Poston L, Sewell RB, Wilkinson SP, Richardson PJ, Williams R, Clarkson EM, MacGregor GA, De Wardener HE (1981) Evidence for a circulating sodium transport inhibitor in essential hypertension. Br Med J 282:847–849
Schoner W, von Ilberg C, Kramer R, Seubert W (1967) On the mechanism of Na+- and K+-stimulated hydrolysis of adenosine triphosphate. I. Purification and properties of Na+- and K+-activated ATPase from ox brain. Eur J Biochem 1:334–343
Thomas RD, Edmondson RPS, Hilton PJ, Jones NF (1975) Abnormal sodium transport in leucocytes from patients with essential hypertension. Clin Sci Mol Med 48:169s-170s
Walter U (1982) ATPase Aktivität und Natriumtransport an Erythrocyten bei essentieller Hypertonie. Klin Wochenschr 60:607–616
Wambach G, Helber A, Bonner G, Hummerich W (1979) Natrium-Kalium-Adenosintriophosphatase-Aktivität in Erythrocytenghosts von Patienten mit essentieller Hypertonie. Klin Wochenschr 57:169–172
Wessels F, Junge-Hülsing G, Losse H (1967) Untersuchungen zur Natriumpermeabilität der Erythrocyten bei Hypertonikern und Normotonikern mit familiärer Hochdruckbelastung. Z Kreislaufforsch 56:374–380
Woods JL, Beevers DG, West M (1981) Familial abnormality of erythrocyte cation transport in essential hypertension. Br Med J 282:1186–1188
Zideck W, Losse H, Dorst KG, Zumkley H, Vetter H (1982) Intracellular sodium and calcium in essential hypertension. Klin Wochenschr 60:859–862
Author information
Authors and Affiliations
Additional information
This paper contains an essential part of the thesis of K.M. presented to the Fachbereich Veterinärmedizin, Giessen
This work was supported by the Deutsche Forschungsgemeinschaft, Bonn-Bad Godesberg (Scho 139/16-2) and by the Fonds der Chemischen Industrie, Frankfurt/Main
Rights and permissions
About this article
Cite this article
Moreth, K., Kuske, R., Renner, D. et al. Blood pressure in essential hypertension correlates with the concentration of a circulating inhibitor of the sodium pump. Klin Wochenschr 64, 239–244 (1986). https://doi.org/10.1007/BF01711656
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01711656