Abstract
The influences of time of storage of plateletrich plasma (PRP), temperature of storage of PRP, platelet number in PRP, mean platelet volume in whole blood, sex, age, hemoglobin concentration, and different forms of PRP storage on platelet aggregation (PAG) tests, performed with epinephrine, collagen, arachidonate, and ristocetin by a four-channel aggregation profiler (Platelet Aggregation Profiler, Model PAP-4, Bio/Data Corporation, Hatboro, PA 19040, U.S.A.), were evaluated in four groups of subjects (52 men, 22 women, age range 20–85 years, hemoglobin concentration range 8.4–16.8 g/dl). The PRP was stored with or without packed cells, at room temperature or at 4° C, for 0–6 h. The ideal platelet number of PRP for performing the PAG test fell between 150 and 500 × 109/l. If the number was less than 150 × 109/l, the result of PAG should be meaningless. No significant change was noted for up to 6 h when the PRP was stored either at room temperature or at 4° C. Hemoglobin concentration and mean platelet volume did not affect the PAG. However, there was significant but weak correlation (p = 0.0125,r = 0.3696) between age and PAG when using arachidonic acid as the agonist. Men had significantly increased PAG when collagen and ristocetin were used as the agonists. The PRP was stored best at room temperature, without packed cells. In conclusion, to obtain the best result from a PAG test, the PRP should be kept without packed cells at room temperature for no longer than 6 h, and the platelet number should fall between 150 and 500 × 109/l.
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References
Born GVR (1962) Aggregation of blood platelets by adenosine diphosphate and its reversal. Nature 194:927–929
Born GVR, Hume M (1967) Effects of the numbers and sizes of platelet aggregates on the optical density of plasma. Nature 215:1027–1029
Cardinal DC, Flower RJ (1980) The electronic aggregometer: a novel device for assessing platelet behaviour in blood. J Pharm Methods 3:135–138
Malpass TW Harker LA (1980) Acquired disorders of platelet function. Semin Hematol 17:242–258
Newhouse P, Clark C (1978) The variability of platelet aggregation. In: Triplett DA (eds) Platelet function: laboratory evaluation and clinical application. Am Soc Clin Path, Chicago, p 63
MacMillan CD, Sim AK (1970) A comparative study of platelet aggregation in man and laboratory animals. Thromb Diath Haemorrh 24:385–394
Sekiya F, Takagi J, Kasahara K, Inada Y, Saito Y (1988) Plasma albumin is essential for collagen-induced platelet aggregation. Thromb Res 50:837–846
Sekiya F, Kawajiri K, Takagi J, Saito Y, Nagase S (1990) Albumin in plasma potentiates platelet aggregation induced by collagen — a study with an albumin-deficient rat. Thromb Res 58:657–662
May J, Loesche W, Heptinstall S (1990) Glucose increases spontaneous platelet aggregation in whole blood. Thromb Res 59:489–495
Moiny G, Thirion A, Deby C (1990) Bilirubin induces platelet aggregation. Thromb Res 59:413–416
The British Society for Haematology — BCSH Haemostasis and thrombosis task force (1988) Guidelines on platelet function testing. J Clin Pathol 41:1322–1330
Remaley AT, Kennedy JM, Laposata M (1989) Evaluation of the clinical utility of platelet aggregation studies. Am J Hematol 31:188–193
Hardeman MR, Vreeken J (1990) The clinical significance of in vitro platelet aggregometry.Thromb Res 59:807–808
Ho CH, Wu SY (1991) The influence of time, temperature and packed cells on activated partial thromboplastin time and prothrombin time. Thromb Res 62:625–633
Hardeman MR, Vreeken J, Goedhart R, Oosting PR (1989) Transient aggregation resistance of human blood platelets in fresh plasma. I. Methodology. Thromb Res 54:719–731
Thompson SG, Kienast J, Pyke SDM, Haverkate F, van de Loo JCW, for The European Concerted Action on Thrombosis and Disabilities Angina Pectoris Study Group (1995) Hemostatic factors and the risk of myocardial infarction or sudden death in patients with angina pectoris. N Engl J Med 332:635–641
Ingerman-Wojenski CM (1984) Simultaneous measurement of platelet aggregation and the release reaction in plateletrich plasma and in whole blood. J Med Technol 1:697–701
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Ho, C.H., Chan, I.H. The influence of time of storage, temperature of storage, platelet number in platelet-rich plasma, packed cell, mean platelet volume, hemoglobin concentration, age, and sex on platelet aggregation test. Ann Hematol 71, 129–133 (1995). https://doi.org/10.1007/BF01702648
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DOI: https://doi.org/10.1007/BF01702648