Abstract
Objective
Development of a stable model of respiratory distress in pigs with oleic acid, fulfilling clinical criteria of the adult respiratory distress syndrome (ARDS).
Design
Eight pigs (9.1±0.7 kg) were anesthetized with pentobarbital, paralyzed with tubocurarine and mechanically ventilated with an\(F_{IO_2 } \) of 0.6, an I∶E ratio of 2∶3 and a PEEP of 0.2 kPa. Oleic acid (dissolved 1∶1 in 96% alcohol) was administered in a series of multiple injections of 0.1 ml until\(P_{aO_2 } \) was lower than 8 kPa.
Measurements and results
Careful titration of the oleic acid injections on guidance of the\(P_{aO_2 } \)established a reproducible respiratory distress (\(P_{aO_2 } \) =7.3±0.8 kPa), in which gas exchange and hemodynamic variables were stable for at least 4 h. The number of oleic acid injections (22±11, mean and SD) varied between the animals.
Conclusions
With the use of multiple injections of oleic acid, a stable model of early respiratory distress in pigs can be achieved, in spite of individual differences in sensitivity. Such a stable model allows for a diversity of studies on early respiratory distress.
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Prof. Dr. C.A. Wagenvoort died on November 23rd, 1995
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Grotjohan, H.P., van der Heijde, R.M.J.L., Jansen, J.R.C. et al. A stable model of respiratory distress by small injections of oleic acid in pigs. Intensive Care Med 22, 336–344 (1996). https://doi.org/10.1007/BF01700456
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DOI: https://doi.org/10.1007/BF01700456