Abstract
Efficient protoplast culture and plant regeneration of five U.S. rice cultivars (Oryza sativa L.) - Mercury, Lacassine, Maybelle, Cypress, and Lemont - were obtained from suspension cells maintained in modified General Medium. Embryogenic suspension cells were developed from calli grown on the original callus induction medium for 10–20 weeks without subculture. Weekly subculture of the suspensions for five to eight weeks yielded cells suitable for protoplast isolation. After 2 weeks, rate of colony formation from protoplasts varied among the cultivars and ranged from 2.5 to 6.8%. Improvement of plating efficiencies to as high as 13.7% was obtained by conducting a second cycle of protoplast culture. A total of 525 plants were regenerated from the cultivars studied.
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Abbreviations
- BAP:
-
6-benzylaminopurine
- CH:
-
casein acid hydrolysate
- MGM:
-
modified General Medium
- Kin:
-
kinetin
- NAA:
-
naphthaleneacetic acid
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
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Communicated by W. Parrott
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Utomo, H.S., Croughan, S.S. & Croughan, T.P. Suspension and protoplast culture of U.S. rice cultivars. Plant Cell Reports 15, 34–37 (1995). https://doi.org/10.1007/BF01690249
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DOI: https://doi.org/10.1007/BF01690249