Abstract
Sixty-six strains unable to ferment sucrose and resemblingVibrio mimicus andV. cholerae were submitted to an extensive phenotypic characterization. DNA-DNA homology among selected strains and the type strain ofV. cholerae was studied by the S1 endonuclease method. Seven sucrose-negative strains were shown to have the phenotypic properties of and a high percentage DNA relatedness toV. cholerae and a low level of homology withV. mimicus. Eight luminescent strains phenotypically most closely resembledV. mimicus; however, two of these were shown to have a high level of DNA homology withV. cholerae and a low level of relatedness toV. mimicus. A single strain was found to be phenotypically and genetically unrelated to eitherV. cholerae orV. mimicus and may represent a new species. The remaining strains were phenotypically shown to beV. mimicus, and selected strains were shown to have a high percentage DNA homology withV. mimicus and a low level of homology withV. cholerae. Problems associated with the identification of these strains and differential traits are discussed.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Literature Cited
Barritt, M. M. 1936. The intensification of the Voges-Proskauer reactions by the addition of α-naphthol. Journal of Pathology and Bacteriology42:441–454.
Bashford, D. J., Donovan, T. J., Furniss, A. L., Lee, J. V. 1979.Vibrio cholerae in Kent. Lancet1:436–437.
Baumann, P., Baumann, L., Mandel, M. 1971. Taxonomy of the marine bacteria: the genus Beneckia. Journal of Bacteriology107:268–294.
Blake, P. A., Donald, M. D., Allegra, D. T., Synder, J. D., Barrett, T. J., McFarland, L., Caraway, C. T., Feeley, J. C., Craig, J. P., Lee, J. V., Puhr, N. D., Feldman, R. A. 1980. Cholera—a possible endemic focus in the United States. New England Journal of Medicine302:305–309.
Colwell, R R., Kaper, J., Seidler, R., Voll, M. J., McNicol, L. A., Garges, S., Lockman, H., Maneval, D., Remmers, E., Joseph, S. W., Bradford, H., Roberts, N., Huq, I., Huq, A. 1980. Isolation of 01 and non-01Vibrio cholerae from estuaries and brackish water environments, pp. 44–56. In: Proceedings of Fifteenth U.S.-Japan Co-operation Medical Science Programme Joint Conference on Cholera, Bethesda, Maryland, 1979.
Crossa, J. H., Brenner, D. J., Falkow, S. 1973. Use of a single strand specific nuclease for analysis of bacterial and plasmid deoxyribonucleic acid homo- and hetero-duplexes. Journal of Bacteriology115:904–911.
Davis, B. R., Fanning, G. R., Madden, J. M., Steigerwalt, A. G., Bradford, H. B., Smith, H. L., Brenner, D. J. 1981. Characterization of biochemically atypicalVibrio cholerae strains and designation of a new pathogenic species,Vibrio mimicus. Journal of Clinical Microbiology14:631–639.
Desmarchelier, P. M., Reichelt, J. L. 1981. Phenotypic characterization of clinical and environmental isolates ofVibrio cholerae from Australia. Current Microbiology5:123–127.
Desmarchelier, P. M., Reichelt, J. L. 1982. Genetic relationships among clinical and environmental isolates ofVibrio cholerae from Australia. Current Microbiology7:53–57.
Edwards, P. R., Ewing, W. H. (eds.) 1972. Identification of Enterobacteriaceae, 3rd edn. Minneapolis, MN: Burgess Publishing Co.
Fanning, G. R., Davis, B. R., Steigerwalt, A. G., Wachsmüth, I. K., Hickman, F. W., Farmer, J. J. III, Brenner, D. J. 1980. Biochemical and genetic parameters ofVibrio cholerae. Abstracts of the Annual Meeting of the American Society for Microbiology.
Furniss, A. L., Lee, J. V., Donovan, T. J. (eds.) 1979. The Vibrios. Public Health Service Monograph Series Number 11. London: Her Majesties Stationary Office.
Heiberg, B. 1936. The biochemical reactions of Vibrios. Journal of Hygiene (Cambridge)36:114–117.
Hugh, R., Feeley, J. C. 1972. Report (1966–1970) of the Subcommittee on Taxonomy of Vibrios to the International Committee on Nomenclature of Bacteria. International Journal of Systematic Bacteriology22:123.
McFaddin, J. F. 1980. Biochemical tests for identification of medical bacteria, 2nd edn. Baltimore: Williams and Wilkins.
Reichelt, J. L., Baumann, P. 1973. Taxonomy of the marine luminous bacteria. Archives of Microbiology94:283–330.
Reichelt, J. L., Baumann, P., Baumann, L. 1976. Study of the genetic relationships among marine species of the generaBeneckia andPhotobacterium by means of in vitro DNA/DNA hybridization. Archives of Microbiology110:101–120.
Rogers, R. C., Cuffe, R. G. C. J., Cossins, Y. M., Murphy, D. M., Bourke, A. T. C. 1980. The Queensland cholera incident of 1977. 2. The epidemiological investigation. Bulletin of the World Health Organization58:665–669.
Sokal, R. R., Sneath, P. H. A. (eds.) 1963. Principles of numerical taxonomy. San Francisco: Freeman.
Stanier, R. Y., Palleroni, N. J., Dondoroff, M. 1966. The aerobic pseudomonads: a taxonomic study. Journal of General Microbiology43:159–271.
Weissman, J. B., De Witt, W. E., Thompson, J., Muchnick, C. N., Portnoy, B. L., Feeley, J. C., Gangorosa, E. J. 1974. A case of cholera in Texas, 1973. American Journal of Epidemiology100:487–498.
West, P. A. 1980. Ecology and taxonomy of the genusVibrio. PhD thesis, University of Kent.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Desmarchelier, P.M., Reichelt, J.L. A phenotypic and genetic study of sucrose nonfermenting strains ofVibrio mimicus andVibrio cholerae . Current Microbiology 10, 41–47 (1984). https://doi.org/10.1007/BF01576046
Issue Date:
DOI: https://doi.org/10.1007/BF01576046