Abstract
Detailed nutrient requirements were determined to maximise efficacy of a sulphate-reducing bacterial mixed culture for biotechnological removal of sulphate, acidity and toxic metals from waste waters. In batch culture, lactate produced the greatest biomass, while ethanol was more effective in stimulating sulphide production and acetate was less effective. The presence of additional bicarbonate and H2 only marginally stimulated sulphide production. The sulphide output per unit of biomass was greatest using ethanol as substrate. In continuous culture, ethanol and lactate were used directly as efficient substrates for sulphate reduction while acetate yielded only slow growth. Glucose was utilised following fermentation to organic acids and therefore had a deleterious effect on pH. Ethanol was selected as the most efficient substrate due to cost and efficient yield of sulphide. On ethanol, the presence of additional carbon sources had no effect on growth or sulphate reduction in batch culture but the presence of complex nitrogen sources (yeast extract or cornsteep) stimulated both. Cornsteep showed the strongest effect and was also preferred on cost grounds. In continuous culture, cornsteep significantly improved the yield of sulphate reduced per unit of ethanol consumed. These results suggest that the most efficient nutrient regime for bioremediation using sulphate-reducing bacteria required both ethanol as carbon source and cornsteep as a complex nitrogen source.
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References
Andrews GF. 1984. Parameter estimation from batch culture data. Biotechnol Bioeng 26: 824–825.
Barnes LJ, FJ Janssen, J Sherren, JH Versteegh, RO Koch and PJH Scheeren. 1991. A new process for the microbial removal of sulphate and heavy metals from contaminated waters extracted by a geohydrological control system. Trans IChemE 69: 184–186.
Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein using the principle of protein-dye binding. Anal Chem 72: 248–254.
Brierley CL. 1990. Bioremediation of metal contaminated surface and ground waters. Geomicrobiol J 8: 201–223.
Catallo WJ and RJ Portier. 1992. Use of indigenous and adapted microbial assemblages in the removal of organic chemicals from soils and sediments. Wat Sci Technol 25: 229–237.
Chang JC. 1993. Solubility product constants. In: CRC Handbook of Chemistry and Physics (Lide DR ed), pp 8–39, CRC Press, Boca Raton.
Cowling SJ, MJ Gardner and DTE Hunt. 1992. Removal of heavy metals from sewage by sulphide precipitation: thermodynamic calculations and tests on a pilot-scale anaerobic reactor. Environ Technol 13: 281–291.
Crathorne B and AJ Dobbs. 1990. Chemical pollution of the aquatic environment by priority pollutants and its control. In: Pollution: Causes, Effects and Control (Harrison RM, ed), pp 1–18, The Royal Society of Chemistry, Cambridge.
Davison AD, H Csellner P Karuso and DA Veal. 1994. Synergistic growth of 2 members from a mixed microbial consortium growing on biphenyl. FEMS Microbiol Ecol 14: 133–146.
Du Preez LA, JP Odendaal, JP Maree and M Ponsonby. 1992. Biological removal of sulphate from industrial effluents using producer gas as energy source. Environ Technol 13: 875–882.
Dvorak DH, RS Hedin, HM Edenborn and PE McIntire. 1992. Treatment of metal-contaminated water using bacterial sulfate reduction: results from pilot-scale reactors. Biotechnol Bioeng 40: 609–616.
Gadd GM and C White. 1993. Microbial treatment of metal pollution—a working biotechnology? Trends Biotechnol 11: 353–359.
Gale NL. 1986. The role of algae and other microorganisms in metal detoxification and environmental clean-up. In: Biotechnology for the Mining, Metal-refining and Fossil Fuel Processing Industries (Ehrlich HL and DS Holmes, eds), pp 171–180, John Wiley and Sons, New York.
Hammack RW and HM Edenborn. 1992. The removal of nickel from mine waters using bacterial sulphate-reduction. Appl Microbiol Biotechnol 37: 674–678.
Hansen TA. 1993. Carbon metabolism in sulfate-reducing bacteria. In: The Sulfate-Reducing Bacteria: Contemporary Perspectives (Odom JM and R Singleton, eds), pp 21–40, Springer-Verlag, New York.
Hedin RS and RW Nairn. 1993. Contaminant removal capabilities of wetlands constructed to treat coal mine drainage. In: Proceedings of the International Symposium on Constructed Wetlands for Water-Quality Improvement (Moshini GA, ed), pp 187–195, Lewis Publishers, Chelsea, MI.
Labib F, JF Ferguson, MM Benjamin, M Merigh and NL Ricker. 1993. Mathematical modelling of an anaerobic butyrate degrading consortium—predicting their responses to organic overloads. Envivon Sci Technol 27: 2673–2684.
Macaskie LE and ACR Dean. 1989. Microbial metabolism, desolubilization, and deposition of heavy metals: uptake by immobilised cells and application to the treatment of liquid wastes. In: Biological Waste Treatment (Mizrahi A, ed), pp 159–201, Alan R Liss, New York.
Madsen T and J Aamand. 1992. Anaerobic transformation and toxicity of trichlorophenols in a stable enrichment culture. Appl Environ Microbiol 58: 557–561.
Maree JP, A Gerber and E Hill. 1987. An integrated process for biological treatment of sulphate-containing industrial effluents. J WPCF 59: 1069–1074.
Odesto P, P Amerlynck, EJ Nyns and HP Naveau. 1992. Acclimatization of a methanogenic consortium to polychlorinated compounds in a fixed-film stationary bed bioreactor. Wat Sci Technol 25: 265–273.
Peck HD. 1993. Bioenergetic strategies of the sulfate-reducing bacteria. In: The Sulfate-Reducing Bacteria: Contemporary Perspectives (Odom JM and R Singleton, eds), pp 41–75, Springer Verlag New York.
Postgate JR. 1984. The Sulphate-Reducing Bacteria. Cambridge University Press, Cambridge.
Scheeren PJH, RO Koch, CJN Buisman, LJ Barnes and JH Versteegh. 1991. New biological treatment plant for heavy metal contaminated groundwater. In: Proceedings of EMC91. Non Ferrous Metallurgy Present and Future, pp 403–416. Elsevier, Amsterdam.
Taylor MRG and RAN McLean. 1992. Overview of clean-up methods for contaminated sites. JIWEM 6: 408–417.
White C, SC Wilkinson and GM Gadd. 1995. The role of microorganisms in biosorption of toxic metals and radionuclides. Int Biodet Biodeg 35: 17–40.
White C and GM Gadd. 1996. Mixed sulphate-reducing bacterial cultures for bioprecipitation of toxic metals: effects of sulphate and substrate concentrations and dilution rate. Microbiology 142: 2197–2205.
Widdel F. 1988. Microbiology and ecology of sulfate- and sulfur-reducing bacteria. In: Biology of Anaerobic Microorganisms (Widdel F, ed), pp 469–585, John Wiley and Sons, New York.
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White, C., Gadd, G.M. A comparison of carbon/energy and complex nitrogen sources for bacterial sulphate-reduction: potential applications to bioprecipitation of toxic metals as sulphides. Journal of Industrial Microbiology 17, 116–123 (1996). https://doi.org/10.1007/BF01570054
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DOI: https://doi.org/10.1007/BF01570054