Summary
Three strains ofSaccharomyces cerevisiae and one strain of aCandida sp. obtained from different industrial sources were screened for uptake of silver and copper. Considerable differences in metal uptake capacities were found between the different strains ofS. cerevisiae and betweenS. cerevisiae and theCandida sp. used. Copper uptake capacities ranged from 0.05 mmol g−1 dry wt to 0.184 mmol g−1 dry wt while values of 0.034 mmol Ag g−1 dry wt and 0.193 mmol Ag g−1 dry wt biomass were observed. Use of ion-selective electrodes (ISEs) enabled the detection of copper complexing agents (possibly proteins and carbohydrates) released by yeasts into the surrounding medium. In contrast, these compounds had no silver complexation abilities. Langmuir and Scatchard transformations of metal adsorption isotherms suggested differences in the mechanisms involved in metal uptake by the various yeasts. The differences between strains ofS. cerevisiae were due possibly to differences in cell wal composition. Different methods of preparation of biomass (fresh, air, oven and freeze-dried) had little effect on metal uptake in comparison with fresh biomass. Storage of fresh waste biomass at 4°C for 20 days had no effect on metal biosorption capacities. It was also observed that individual batches of waste biomass produced from different fermentation runs had consistent metal uptake capacities. The implications of the above results on the use of waste yeast biomass for treatment of metal-containing effluents are discussed.
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References
Analytical Methods Committee. 1967. The use of 50 per cent hydrogen peroxide for the destruction of organic matter. The Analyst 92: 403–407.
Ashley, N.V. and D.J.W. Roach. 1990. Review of biotechnology applications to nuclear waste treatment. J. Chem. Technol. Biotechnol. 49: 381–394.
Avery, S.V. and J.M. Tobin. 1992. Mechanisms of Sr uptake by laboratory and brewing strains ofSaccharomyces cerevisiae. Appl. Environ. Microbiol. 58: 3883–3889.
Avery, S.V. and J.M. Tobin. 1993. Mechanism of adsorption of hard and soft metal ions toSaccharomyces cerevisiae and influence of hard and soft anions. Appl. Environ. Microbiol. 59: 2851–2856.
Cabral, J.P.S. 1992. Limitations on the use of an ion selective electrode in the study of the uptake of Cu2+ byPseudomonas syringae cells. J. Microbiol. Meth. 16: 149–156.
De Rome, L. and G.M. Gadd. 1987. Copper adsorption byRhizopus arrhizus, Cladosporium resinae andPenicillium italicum. Appl. Microbiol. Biotechnol. 26: 84–90.
Failla, M.L., C.D. Benedict and E.D. Weinberg. 1976. Accumulation and storage of Zn2+ byCandida utilis. J. Gen. Microbiol. 94: 23–36.
Gadd, G.M. 1992. Microbial control of heavy metal pollution. In: Microbial Control of Pollution (Fry, J.C., G.M. Gadd, C.W. Jones, I. Watson-Craik, eds), pp. 59–88, Cambridge University Press, Cambridge.
Gadd, G.M. 1993. Interactions of fungi with toxic metals. New Phytol. 124: 25–60.
Gadd, G.M. and J.L. Mowll. 1983. The relationship between cadmium uptake, potassium release and viability inSaccharomyces cerevisiae. FEMS Microbiol. Lett. 16: 45–48.
Garnham, G.W., G.A. Codd and G.M. Gadd. 1992. Kinetics of uptake and intracellular location of cobalt, manganese and zinc in the estuarine green algeChlorella vulgaris. Appl. Microbiol. Biotechnol. 37: 270–276.
Harris, P.O. and G.J. Ramelow. 1990. Binding of metal ions by particulate biomass derived fromChlorella vulgaris andScenedesmus quadricauda. Environ. Sci. Technol. 24: 220–228.
Herbert, D., P.J. Phipps and R.E. Strange. 1971. Chemical analysis of microbial cells. Meth. Microbiol. 5: 209–344.
Huang, C.-P., C.-P. Huang and A.L. Morehart. 1990. The removal of Cu(II) from dilute aqueous solution bySaccharomyces cerevisiae. Water Res. 24: 433–439.
Hughes, M. and R. Poole. 1991. Metal speciation and microbial growth—the hard (and soft) facts. J. Gen. Microbiol. 137: 725–734.
Hunt, S. 1986. Diversity of biopolymer structure and its potential for ion-binding applications. In: Immobilization of Ions by Biosorption (Eccles, H. and Hunt, S., eds), pp. 15–46, Ellis-Horwood, Chichester.
Luef, E., T. Prey and C. Kubicek. 1991. Biosorption of zinc by fungal mycelial wastes. Appl. Microbiol. Biotechnol. 34: 688–692.
Nieboer, E. and D.H.S. Richardson. 1980. The replacement of the nondescript term ‘heavy metal’ by a biologically and chemically significant classification of metal ions. Environ. Pollut. Ser. B. 1: 3–26.
Parkin, M.J. and I.S. Ross. 1985. Uptake of copper and manganese by the yeastCandida utilis. Microbios. Lett. 29: 115–120.
Pearson, R.G. 1963. Hard and soft acids and bases. J. Am. Chem. Soc. 85: 3533–3539.
Phaff, H.J. 1963. Cell wall of yeasts. Ann. Rev. Microbiol. 17: 15–30.
Speers, R.A., M.A. Tung, T.D. Durance and G.G. Stewart. 1992. Biochemical aspects of yeast flocculation and its measurement: a review. J. Inst. Brew. 98: 293–300.
Tobin, J.M., D.G. Cooper and R.J. Neufeld. 1990. Investigation of the mechanism of metal uptake by denaturedRhizopus arrhizus biomass. Enz. Microb. Technol. 12: 591–595.
Treen-Sears, M.E., S.M. Martin and B. Volesky. 1984. Propagation ofRhizopus javanicus biosorbent. Appl. Environ. Microbiol. 48: 137–141.
Tsezos, M. and B. Volesky. 1982. The mechanism of thorium biosorption byRhizopus arrhizus. Biotechnol. Bioeng. 24: 955–969.
Venkateswerlu, G. and G. Stotzky. 1989. Binding of metals by cell walls ofCunninghamella blakesleeana grown in the presence of copper or cobalt. Appl. Microbiol. Biotechnol. 31: 619–625.
Volesky, B., H. May and Z. Holan. 1993. Cadmium biosorption bySaccharomyces cerevisiae. Biotechnol. Bioeng. 41: 826–829.
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Simmons, P., Tobin, J.M. & Singleton, I. Considerations on the use of commercially available yeast biomass for the treatment of metal-containing effluents. Journal of Industrial Microbiology 14, 240–246 (1995). https://doi.org/10.1007/BF01569934
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DOI: https://doi.org/10.1007/BF01569934