Summary
A comparison of transmembrane potential (pd) properties of parenchyma cells and giant transfer cells induced by a root-knot nematode in the roots ofImpatiens balsamina has been made. Apart from some differences in rate of response to a few treatments, parenchyma and giant cells had similar pd values; active and passive components of the pd (cyanide, azide); responses to total ion concentration, pH and potassium concentration; responses to protein synthesis inhibitors (puromycin, cycloheximide and actinomycin D) and responses to sugars.
Both parenchyma cells and giant cells are depolarized by puromycin, cycloheximide and actinomycin D. The cells recover from the depolarization in the presence of cycloheximide, suggesting that this presumed protein synthesis inhibitor does not act in a straight-forward manner. The cells do not recover in the presence of puromycin or actinomycin D.
Parenchyma cells and giant cells clearly have different metabolic rates and ion fluxes, but their pd responses are the same. This suggests that the pd does not reflect metabolic activity or ion fluxes of a cell, but is strictly controlled in itself. Part of this control may be via a feedback mechanism acting on an electrogenic pump.
The depolarization caused by glucose is induced by aging the cells after excision. The effect is discussed in terms of an H+ dependent cotransport system and an ATPase permease system.
The apparent normality of pd responses of nematode-induced giant transfer cells suggests that they may be a useful model system for experiments on higher plant cells.
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Anderson, W. P., D. L. Hendrix, andN. Higinbotham, 1974: Higher plant cell membrane resistance by a single intracellular electrode method. Plant Physiol.53, 122–124.
Bird, A. F., 1961: The ultrastructure and histochemistry of a nematode induced giant cell. J. biophys. biochem. Cytol.11, 701–715.
Bowling, D. J. F., 1972: Measurement of potassium activity and electrical potential in the intact root. Planta (Berl.)108, 147–151.
—, 1973: A pH gradient across the root. J. exp. Bot.24, 1041–1045.
Cleland, R., 1973: Auxin-induced hydrogen ion excretion fromAvena coleoptiles. Proc. nat. Acad. Sci.70, 3092–3093.
Dropkin, V. H., andP. E. Nelson, 1960: The histopathology of root-knot nematode infections in soybeans. Phytopathology50, 442–447.
Ellis, R. J., andI. R. MacDonald, 1970: Specificity of cycloheximide in higher plant systems. Plant Physiol.46, 227–232.
Endo, B. Y., andJ. A. Veech, 1969: The histochemical localization of oxido-reductive enzymes of soybeans infected with the root-knot nematode,Meloidogyne incognita acrita. Phytopathology59, 418–425.
Etherton, B., 1963: Relationship of cell transmembrane electropotential to potassium and sodium accumulation ratios in oat and pea seedlings. Plant Physiol.38, 581–585.
—, andN. Higinbotham, 1960: Transmembrane potential measurements of cells of higher plants as related to salt uptake. Science131, 409–410.
—, andG. J. Nuovo, 1974: Rapid changes in membrane potentials of oat coleoptile cells induced by amino acids and carbohydrates. Plant Physiol. (suppl.), 49.
Franklin, T. J., and G. A.Snow, 1971 : Biochemistry of antimicrobial action. Academic Press, pp. 1–163.
Gayler, K. R., andK. T. Glasziou, 1972: Physiological functions of acid and neutral invertases in growth and sugar storage in sugar cane. Physiol. Plant27, 25–31.
Guy, M., andL. Reinhold, 1974: The uptake of 2-deoxy-D-glucose by isolatedRicinus cotyledons. Physiol. Plant.31, 4–10.
Hager, A., H. Menzel, undA. Krauss, 1971: Versuche und Hypothese zur Primärwirkung des Auxins beim Streckungswachstum. Planta (Berl.)100, 47–75.
Higinbotham, N., 1973: Electropotentials of plant cells. Ann. Rev. Plant Physiol.24, 25–46.
—,B. Etherton, andR. J. Foster, 1964: Effect of external K, NH4, Na, Ca, Mg and H ions on the cell transmembrane electropotential ofAvena coleoptile. Plant Physiol.39, 196–203.
—,J. S. Graves, andR. F. Davis, 1970: Evidence for an electrogenic ion transport pump in cells of higher plants. J. Membrane Biol.3, 210–222.
Hill, B. S., andA. E. Hill, 1973: ATP-driven chloride pumping and ATPase activity in theLimonium salt gland. J. Membrane Biol.12, 145–158.
Jones, M. G. K., 1974: Scanning electron microscopy of transfer cell wall ingrowths in nematode induced giant cells. Plant Physiol. (suppl.) 15.
- and V. H.Dropkin, 1975: Cellular alterations induced in soybean roots by three endoparasitic nematodes. Physiol. Plant. Path. (in press).
—, andD. H. Northcote, 1972: Multinucleate transfer cells induced in coleus roots by the root-knot nematode,Meloidogyne arenaria. Protoplasma75, 381–395.
—,A. Novacky, andV. H. Dropkin, 1974: “Action potentials” in nematode-induced plant transfer cells. Protoplasma80, 401–405.
Kitasato, H., 1968: The influence of H+ on the membrane potential and ion fluxes ofNitella. J. gen. Physiol.52, 60–87.
Lüttge, V., andC. K. Pallaghy, 1969: Light triggered transient changes of membrane potential in green cells in relation to photosynthetic electron transport. Z. Pflanzen-physiol.61, 58–67.
Mitchell, P., 1966: Chemiosmotic coupling in oxidative and photosynthetic phosphorylation. Biol. Rev.41, 445–502.
Owens, R. G., andJ. H. Rubinstein, 1966: Metabolic changes induced by root-knot nematodes in host tissue. Contrib. Boyce Thompson Inst.23, 199–213.
—, andH. N. Specht, 1966: Biochemical alterations induced in host tissues by root-knot nematodes. Contrib. Boyce Thompson Inst.23, 181–198.
Pate, J. S., andB. E. S. Gunning, 1972: Transfer cells. Ann. Rev. Plant. Physiol.23, 173–196.
Paulson, R. E., andJ. M. Webster, 1970: Giant cell formation in tomato roots caused byMeloidogyne incognita andMeloidogyne hapla (Nematoda) infection. Can. J. Bot.48, 271–276.
Penny, M. G., andD. J. F. Bowling, 1974: A study of potassium gradients in epidermis of intact leaves ofCommelina communis L. in relation to stomatal opening. Planta (Berl.)119, 17–25.
Rashke, K., andG. D. Humble, 1973: No uptake of anion required by opening stomata ofVicia faba: Guard cells release hydrogen ions. Planta (Berl.)115, 47–57.
Raven, J. A., and F. A.Smith, 1973: The regulation of intracellular pH as a fundamental biological process. In: Ion transport in plants (W. P.Anderson, ed.), pp. 271–278. Academic Press.
— —, 1974: Significance of hydrogen ion transport in plant cells. Can. J. Bot.52, 1035–1048.
Sacher, J. A., M. D. Hatch, andK. T. Glasziou, 1963: Sugar accumulation cycle in sugar cane. III. Physical and metabolic aspects of cycle in immature storage tissues. Plant Physiol.38, 348–354.
Slayman, C. L., 1965: Electrical properties ofNeurospora crassa: respiration and the intracellular potential. J. gen. Physiol.49, 93–116.
—,W. S. Long, andC. Y.-H. Lu, 1973: The relationship between ATP and an electrogenic pump in the plasma membrane ofNeurospora crassa. J. Membrane Biol.14, 305–338.
—, andC. W. Slayman, 1974: Depolarization of the plasma membrane ofNeurospora during active transport of glucose: evidence for a protondependent cotransport system. Proc. nat. Acad. Sci.71, 1935–1939.
Spanswick, R. M., 1972: Evidence for an electrogenic ion pump inNitella translucens. 1. The effect of pH, K+, Na+, light and temperature on the membrane potential and resistance. Biochem. biophys. Acta288, 73–89.
Veech, J. A., andB. Y. Endo, 1969: The histochemical localization of several enzymes in soybean infected with the root-knot nematodeMeloidogyne incognita acrita. J. Nematology1, 265–276.
— —, 1970: Comparative morphology and enzyme histochemistry in root-knot resistant and susceptible soybeans. Phytopathology60, 896–902.
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Jones, M.G.K., Novacky, A. & Dropkin, V.H. Transmembrane potentials of parenchyma cells and nematode-induced transfer cells. Protoplasma 85, 15–37 (1975). https://doi.org/10.1007/BF01567756
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DOI: https://doi.org/10.1007/BF01567756