Abstract
The localization of type X collagen and alkaline phosphatase activity was examined in order to gain a better understanding of tissue remodelling during development of human first rib cartilage. First rib cartilages from children and adolescents showed no staining for type X collagen and alkaline phosphatase activity. After onset of mineralization in the late second decade, a peripheral ossification process preceded by mineralized fibrocartilage could be distinguished from a more central one preceded by mineralized hyaline cartilage. No immunostaining for type X collagen was found in either type of cartilage. However, strong staining for alkaline phosphatase activity was detected around chondrocyte-like cells within fibrocartilage adjacent to the peripheral mineralization front, while a weaker staining pattern was observed around chondrocytes of hyaline cartilage near the central mineralization front. In addition, the territorial matrix of some chondrocytes within the hyaline cartilage revealed staining for type I collagen, suggesting that these cells undergo a dedifferentiation process, which leads to a switch from type II to type I collagen synthesis. The study provides evidence that mineralization of the hyaline cartilage areas in human first rib cartilage occurs in the absence of type X collagen synthesis but in the presence of alkaline phosphatase. Thus, mineralization of first rib cartilage seems to follow a different pattern from endochondral ossification in epiphyseal discs.
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Bonucci E, Silvestrini G, Bianco P (1992) Extracellular alkaline phosphatase activity in mineralizing matrices of cartilage and bone: ultrastructural localization using a cerium-based method. Histochemistry 97:323–327
Burstone MS (1962) Enzyme histochemistry and its application in the study of neoplasms. Academic Press, New York
Castagnola P, Moro G, Descalzi-Cancedda F, Cancedda R (1986) Type X collagen synthesis during in vitro development of chick embryo tibial chondrocytes. J Cell Biol 102:2310–2317
Dearden LC, Bonucci E, Cuicchio M (1974) An investigation of ageing in human costal cartilage. Cell Tissue Res 152:305–337
Galotto M, Campanile G, Robino G, Descalzi Cancedda F, Bianco P, Cancedda R (1994) Hypertrophic chondrocytes undergo further differentiation to osteoblast-like cells and participate in the initial bone formation in developing chick embryo. J Bone Miner Res 9:1239–1249
Gibson GJ, Flint MH (1985) Type X collagen synthesis by chick sternal cartilage and its relationship to endochondral development. J Cell Biol 101:277–284
Gibson GJ, Kielty CM, Garner C, Schor SL, Grant ME (1983) Identification and partial characterization of three low-molecular-weight collagenous polypeptides synthesized by chondrocytes cultured within collagen gels in the absence and in the presence of fibronectin. Biochem J 211:417–426
Grant WT, Wang G-J, Bahan G (1987) Type X collagen synthesis during endochondral ossification in fracture repair. J Biol Chem 262:9844–9849
Heinrich A (1941) Alternsvorgänge im Röntgenbild. Thieme, Leipzig, pp 61–71
Holmdahl R, Rubin K, Klareskog L, Larsson E, Wigzell H (1986) Characterization of the antibody response in mice with type II collagen-induced arthritis, using monoclonal anti-type II collagen antibodies. Arthritis Rheum 29:400–410
Iyama KI, Ninomiya Y, Olsen BR, Linsenmayer TF, Trelstad RL, Hayashi M (1991) Spatiotemporal pattern of type X collagen gene expression and collagen deposition in embryonic chick vertebrae undergoing endochondral ossification. Anat Rec 229:462–472
Jacenko O, LuValle PA, Olsen BR (1993) Spondylometaphyseal dysplasia in mice carrying a dominant negative mutation in a matrix protein specific for cartilage-to-bone transition. Nature 365:56–61
Kampen WU, Claassen H, Kirsch T (1995) Mineralization and osteogenesis in the human first rib cartilage. Ann Anat 177:171–177
Kirsch T, Mark K von der (1991) Isolation of human type X collagen and immunolocalization in fetal human cartilage. Eur J Biochem 196:575–580
Koebke J, Saternus KS (1985) Untersuchungen zur Verkalkung und Ossifikation des Rippenknorpels. Verh Anat Ges 79:267–269
Mark H von der, Mark K von der, Gay S (1976) Study of differential collagen synthesis during development of the chick embryo by immunofluorescence. I. Preparation of collagen type I and type II specific antibodies and their application to early stages of the chick embryo. Dev Biol 48:237–249
Mark K von der (1986) Differentiation, modulation and dedifferentiation of chondrocytes. Rheumatology 10:272–315
Nerlich AG, Kirsch T, Wiest I, Betz P, Mark K von der (1992) Localization of collagen X in human fetal and juvenile articular cartilage and bone. Histochemistry 98:275–281
Olah AJ, Simon A, Gaudy M, Herrmann W, Schenk RK (1977) Differential staining of calcified tissues in plastic embedded microtome sections by a modification of Movat's Pentachrome stain. Stain Technol 52:331–337
Poole AR, Pidoux I (1989) Immunoelectron microscopic studies of type X collagen in endochondral ossification. J Cell Biol 109:2547–2554
Reichenberger E, Aigner T, Mark K von der, Stöß H, Bertling W (1991) In situ hybridization studies on the expression of type X collagen in fetal human cartilage. Dev Biol 148:562–572
Romeis B (1989) Mikroskopische Technik. Böck P (ed). Urban and Schwarzenberg, Munich, p 501
Schmid TM, Conrad HE (1982) A unique low molecular weight collagen secreted by cultured chick embryo chondrocytes. J Biol Chem 257:12444–12450
Schmid TM, Linsenmayer TF (1985) Immunohistochemical localization of short chain cartilage collagen (type X) in avian tissues. J Cell Biol 100:598–605
Schmid TM, Linsenmayer TF (1987) Type X collagen. In: Main R, Burgeson RE (eds) Structure and function of collagen types. Academic Press, London, pp 223–259
Schmid TM, Linsenmayer TF (1990) Immunoelectron microscopy of type X collagen: supramolecular forms within embryonic chick cartilage. Dev Biol 138:53–62
Werner B (1978) Röntgenologisch darstellbare Veränderungen der knorpligen Anteile des ersten Rippenpaares unter Berücksichtigung von Alter, Geschlecht und den knorpligen Anteilen der übrigen Rippen. Anat Anz 144:319–333
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Claassen, H., Kampen, W.U. & Kirsch, T. Localization of collagens and alkaline phosphatase activity during mineralization and ossification of human first rib cartilage. Histochem Cell Biol 105, 213–219 (1996). https://doi.org/10.1007/BF01462294
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DOI: https://doi.org/10.1007/BF01462294